#NEXUS
[!This data set was downloaded from TreeBASE, a relational database of phylogenetic knowledge. TreeBASE has been supported by the NSF, Harvard University, Yale University, SDSC and UC Davis. Please do not remove this acknowledgment from the Nexus file.


Generated on September 18, 2025; 21:25 GMT

TreeBASE (cc) 1994-2008

Study reference:
Cho S., Valles Y., & Weisblat D. 2013. Differential expression of conserved germ
line markers, and  delayed segregation of male and female primordial germ  cells,
in a hermaphrodite, the leech Helobdella. Molecular Biology and Evolution, .

TreeBASE Study URI:  http://purl.org/phylo/treebase/phylows/study/TB2:S14911]

BEGIN TAXA;
        TITLE  Taxa;
        DIMENSIONS NTAX=28;
        TAXLABELS
            Bombyx_mori_Arg1
            Bombyx_mori_Arg2
            Bombyx_mori_Piwi
            Capitella_teleta_Arg1
            Capitella_teleta_Piwi1
            Capitella_teleta_Piwi2
            Helobdella_robusta_Arg1
            Helobdella_robusta_Arg2
            Helobdella_robusta_Piwi1
            Helobdella_robusta_Piwi2
            Lottia_gigantea_Arg
            Lottia_gigantea_Piwi1
            Lottia_gigantea_Piwi2
            Mus_musculus_Arg1
            Mus_musculus_Arg2
            Mus_musculus_Arg3
            Mus_musculus_Arg4
            Mus_musculus_Piwi1
            Mus_musculus_Piwi2
            Nematostella_vectensis_Arg1
            Nematostella_vectensis_PiwiA
            Nematostella_vectensis_PiwiB
            Strongylocentrotus_purpuratus_Arg1
            Strongylocentrotus_purpuratus_Seawi
            Tribolium_castaneum_Arg1
            Tribolium_castaneum_Arg2a
            Tribolium_castaneum_Arg2b
            Tribolium_castaneum_Piwi
            ;
END;

BEGIN CHARACTERS;
[! TreeBASE Matrix URI: http://purl.org/phylo/treebase/phylows/matrix/TB2:M19425]

	TITLE 'Piwi-Argonaute';
	LINK TAXA = Taxa;
	DIMENSIONS NCHAR=512;
	FORMAT DATATYPE=Protein SYMBOLS= "A C D E F G H I K L M N P Q R S T V W Y" MISSING=? GAP= -;

MATRIX
[                                               10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       450       460       470       480       490       500       510  ]
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Bombyx_mori_Arg1                        GHEGRPIMLRANHFHHYDVNIQPDKCPRFDGRNNLYTRDPLPDRVFRVTIKWVAQVLALDVVMRLPSMMYTPVGRSFFSSPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGTMKRKYRSFPLQLENGTVAKYFLDKYYLPLEVCNIVPGQRCIKKLTDMQTSTMIKATARSAPD-REREINNLGRVLPPPKLQYGW--DMRGFTQQLQKISNDAGMPIDQVEPMFKYLQLVVVVLP-GKTPVYAEVKRVGDTVLGMATQCVQAKQTLSNLCLKINVKLGGIN-SILVPIFLGVDVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEVHEMSSMVQELLIMFYPHRIIMYRDGISEGQFLHVLQHELTAVREAYKPGITFIVVQKRHHTRLFNIPAGTTVDLGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFGSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
Bombyx_mori_Arg2                        SPPSRTVPILTNYLYRYDVTFKPDK-PKFDQTKNCYSLTPLPDMPFEVSFKASGIVQCIDIVLKGTLESYVKAGRQYFMRPDLGDGLEMWTGLFQSFINVDVAHKGFPKNQPMPGRAAE--AFNEFIRGKILGTGPSSGQLRTFTLENDKGTVYEYFMKEKYLPMELVEVAYGQARNKQLNDRQLSTMVREAA-TPPDVRKRKIEEVAKILEAPTLEVGW----QAIVSKLMNTGRQMGMNVDLHKSMLHALNFLVVVVSGRGRDYYHKLKQIAELKVGILTHVFKEDQTARNILLKVNSKLMGINQALENRMIVGADVTHPSPDQSNIPSIAAVTASMDTKCYIYNIELSIQTPKKEIVQFEDIMVDHFHAFKPKKVFVFRDGVSEGQFAEVMKSELTGLHRAAKPEVLFILVQKRHHTRFFNVDPGTVVDRDIVHPRELDFYLVSHQAIKGTARPTRYHAVCNDGRIPENEVEHLAYYLCHLYARCMRAVSYPAPTYYAHLACLRARSLT
Bombyx_mori_Piwi                        GTSGTPLDLLANYFYQYHVDISPEEDSTFDGTV-LYTVNRLHNERMRILIKLTCEVQIFNIIIRFNLLKLQLMGRDYFDPEP-EFKLQIWPGYKTTLLVTEIAHKVL-RMDTVTKGNNYKKIFLEDVVGKIVMTDYNKRTYRTFKMRDEN-TYIEYYYKKYYLVPELCRQTGLSDEMR--ANFKLMRSLDVHTKIGPDKRIEKLNNFGRQLPPENIIQAWTRDMRSFIDLIIKTGGGVGFRMIEYANMCEEVALILCVLARNYADRYEAIKKKCTVDRAVPTQVVCARSIATKVAIQINCKLGGSPWTVDIPMVVGYDVCHDTRSKEK--SFGAFVATLDKQMTQYYSIVNAHTSGEESSHMGFNIASAVKKFRPARIFIYRDGVGDGQIPYVHSHEVAEIKKKVEIKLAFIIVSKRINTRIFNPRPGTVIDDVVTLPERYDFYLVSQNVREGTIAPTSYNVIEDTTGLNPDRIQRLTYKLTHLYFNCSSQVRVPSVCQYAHKLAFLAANSL
Capitella_teleta_Arg1                   GVEGRHIMLRANHFHHYEISIQPDKCPRFDGRKNLYCRDPLPDRVFKVSIRWMAQVQALDVVMRLPSMTYTPVGRSFFSPPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGTMKRKYRSFPLQLESGTVAKYFLERYYLPLEVCNIVGGQRCIKKLTDMQTSTMIKATARSAPD-REKEINSLGRVLPAPKLQYGW--DMRGFTQQLQRISNDAGMPIDQVEPMFRYLQLIVVVLP-GKTPVYAEVKRVGDIMFGLATQCVQSKQTLSNLCLKINVKLGGIN-NILLPIFLGADVTHPPVGDRSKPSIAAVVGSMDAHPSRYSATVRVQSHRQEISELSSMVKELLIQFYPYRIIFYRDGVSEGQFTQVLHHELRAVREAYHPGITFIVVQKRHHTRLFNIPAGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFSADELQSLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
Capitella_teleta_Piwi1                  GDYGNRVCMYTNTFCQYHVNYTPPVESRFDGMI-LYLGRKLDGDNIKVSIEFTGEVQILNIMMRLGALGMKLIGKNYFMPSP-QYGYEIYPGYTTAQLICDVAHKIL-RPHSVARGGNFHENCTKKLVGEIVMTTYNNKTYRTFKQR--DGTFNEYYKKQYLLVPELCRLTGISEEAR--ADFMVMKNLSVFTRVSPQGRMERLIEFGRNLGCERLVQGWSREMRGLHETLRRVSGAMGMRLGYTQTFINALQLVVCVLPSNKKDLYDSIKKFCCCEKPVPSQCIIQRSVCTKVAVQMNCKLGGEVWAVDIPMVIGIDTYHDSAQKGR--SIGAFVASTNNTLTKYYSRCCFQTTGQEMSGLQICMRDALKHYMPERVFIYRDGVGDGQVDHVANSEVKQIMECYAPKTAVIIVTKRINNRFFNPLPGTVVDTVVTRPEWYDFFLVSQSVRQGTVSPTHYNVVHDSSGMSPNHLQRLTYKLCHLYYNWPGTIRVPAPCQYAHKLAFLVGQSL
Capitella_teleta_Piwi2                  -----------MCF------------RS------------------------------------QPMLVHVPKKKDIQRQP---------------------------RGKPL----------------------------R-------------------LLVPEFCFLTGLSDSMK--ADFRVTKDLATHTRITPAQRHLSIRKFGRGIRPLDIVFGWGRQLGRFVQVMMQEAPKMGIRVVRNESYISQLQLVVCLMPRGRDDLYSAVKTLCCHEQPVPSQMIVSRSVVQKVILQINAKLGGELWSVAMPMVCGIDVYHDKLSGK---SIAALVSSMNPQCTRWYSRTCEQMSNQEVDSLGPCFVAAIKKFAPNRIVLFRDGVGDGQLAHVSQHEAAQLEKCYKPKLSIVVVQKRINQRIFNPPPGTVVDHTITRFNWYDFFLVSQHVRQGTVSPTHYVVVHDDSGWDCDRMQRLSYMLTHLYYNWPGTVRVPAPCQYAHKLAYLQGQNL
Helobdella_robusta_Arg1                 GNEGQPICLRANHFNHYDVTISPDKCPRFDGRKNLYSRDQLPDRVFHVSIKFVAQVQALDVVMRLSSMTFQ--GRSFFSPPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGAMKRKYRSFPLQLESGTVAKYFQERYYLPLEVCNIVHGQRCIKKLTDLQTSTMIKATARSAPD-REKEINNLGRILPVPKLQYGW--DMRGFTQQLQKISTDAGMPIDQVEPMFRYLQLIVVVLP-GKTPVYAEVKRVGDILFGLATQCVQSKQTISNLCLKINVKLGGIN-SILLPIFLGADVTHPPAGDRSKPSIAAVVGSMDAHPSRYSATVRIQQHRQEIQELSAMVKELLIQFYPTRIIFYRDGVSEGQFAHVLSYELRAIREAYQPGISFIVVQKRHHTRLFNIPAGTTVDIGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTADELQMLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
Helobdella_robusta_Arg2                 GSEGRKILLKANHFYHYDVAVMPDKCPRYDGRRNMYSKEPLMERVFKVTI------------------RYSAANAQVMFNPTLGG----------------VSATAFYKAQPVPLSDAQRVKFTKEIRG-------------DFPLQLERGTVAKYFLEKYYLPLEVCNIVHGQKCIKKLTDSQTSTMIKSTSRAAPD-REREINTLGRVLPVPKLQM--------FTQQLQKISTDAGMPVDQI-------TFLF-----AKSSSPSEVKRVGDILFGLATQCVQSKQTLSNLCLKIN-------------IFLGADVTHPPAGDRSKPSIAAVVGSMDAHPSRYSATVRIQQHRQEIQDLSVM---------PCRY----------------------------PGGSFV-----------------------------------HSSIH------------------------------------------------------------
Helobdella_robusta_Piwi1                GLSGTRLTICTNLFFQYHVDFVPDIPNIFDGSI-LFTSHRIDGEDIKISIRYTNAVQIYNLIFKLREIGLQIVGRNYFNPQR-ELGLKVWPGYFTSNLMVDICHKVL-GQETVLRPRSYVEDSKRDVVGKIVLTRYNNRTYRTFPMR--DGSFKEYYRTRHCLIPELCIVTGISDEIK--SNFHSMRELSTHTRLGPHERSGKIEQLARTLEAEQIFLGWSRDMRAFFTKLVQAGQMMGVKIESNRSYIQTLQLVMCVLESEKKDKYDVIKKTLCINVPIPSQVMLMKSVATKVVIQVNCKVGGEGWAVSIPMVIGIDTYHDSSKKYR--SVVGIVGSTNSSFTKYHTTWAFQEKGEESSCLCAAIASLVKAFTPGRIFVYRDGVGDGQLGMVYDHELLQIQEGYKPKITLFVVKKRVNNRFFNPPPGTVIDTKATRPHWYDFFLISQSVNQGTVTPTHYNILHDDSGLKPDHLQRLTYKLCHLYFNWQGTVRVPAPCQYAHKVAFLIGQSL
Helobdella_robusta_Piwi2                GSTGKKVPLCANYIYQYYVSFEPIVDCSFDGMT-LYLPKRLEGVPIIISFKLVKIVHLYNIIFKMRALNMCVIGRNYYMPSP-KEKLEVWPGYVTSMLLADVSHRVL-RTQTASHQQTCQDDISKLLVGNVVLTRYNNKCYRTFSLY--NGTFAEYYKRMYCLVPELSYITGLTDTMR--ADNRVMRELASHTRLNPTQRRDALRTYGRLLPIETIRFRWSRAVTKFMSMYRQCSRDMGIRVDRTESYMREIQLVVTIFPFQREDRYSAIKQLCCHDMPIPSQVINSRSVTQKIALQINCKLGGELWALNIPMVCGVDVTHDPAHHKS--SVVGFVSNINQTCTRWYSKVRMQQPGEEVNGLKICFTDALRKYYPSRVVIYRDGVGDGQLSVVRDYEVKQLIECCKPKLGVIVVQKRINTRLLNPRPGTVVDHTVTRNNWYDFFVVSQHIRQGTVSPTHYVVVYDGLDWKPDIVQALTYKMTHLYYNWPGTVRVPAPCQYAHKLAFLIGQNV
Lottia_gigantea_Arg                     GTEGRPILLRANHFHHYDVSITPDKCPRFDGRKNLYSREPLPDRVFTVAIKWVAQVQALDVVMRLPSMTYTPVGRSFFSQPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGTMKRKYRSFPLQLDSGTVARYFHEKYYLPLEVCNIVGGQRCIKKLTDMQTSTMIKATARSAPD-REKEINNLGRVLTPPKIQYGW--DMRGFTSQLQRISNDAGMPIDQVEPMFRYLQLIVVVLP-GKTPVYAEVKRVGDILFGLATQCVQSKQTLSNLCLKINVKLGGIN-SILLPIFLGADVTHPPAGDCMKPSIAAVVGSMDAHPSRYSATVRVQQHRQEIPELCNMIRELLIQFYPTRIIFYRDGVSEGQFQTVLSSELRAVREAYQPGITFIVVQKRHHTRLFNIPAGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
Lottia_gigantea_Piwi1                   GSAGKPIALLANFFYQYQVDFNPSIDSRFDGGI-LYLPKKLQDTKIQITITLTNELQVVNIIFRLAAIEMKEIGRHYFNPSP-RHKLSVMPGFATSLLGADIAHKII-RTDSVRGGGSFYDKVFKALVGSIVLTRYNNKTYRKFKLH--NGSYVDYYKKNYYLLPELCTLTGLSDDVR--ADFSVMKDVATHTRIAPEGRCKKLTDFGRVLPPEAISQGWSRDMRGLVDTLIRVGGPMGMRIDRNETFLETLQMVLIVVPNNKKDRYDAIKKFCCVEHPVPSQVVVARSVATKICIQMNCKLGGEVWTVEIPMVIGIDTYHDSSQKGK--SVGGVIASLNTNLTRFYSTVTHQTQHQEIDGLVASLNGALRKYHPEKIIVYRDGVGDGQLPTVFGHEVPQLLEAYNPKLAVIVVKKRINARFFNPVPGTCVDNEVTKPEWYDFFLVSQSVRQGTVTPTHYNVIWDQTGLKPDHMQRLSYKLTHLYYNWPGTIRVPAPCQYAHKLAFLVGQSL
Lottia_gigantea_Piwi2                   --------MSANYIYQYHVSYNPQIDSKFDGAL-LFLPKKLPGADITLTIKLIKVVQVYNILLRMSILELCQVGRYYYNPHP-QHKLEVWPGYITAMLLLDASHRVL-RTETVNNPKGFKDEITKLVLGNIVLTRYNNKTYKTFPAS-KTGSFVDYYKTNYCLVPELCYMTGLSDAMR--SDFRLMKDIASHTRVTPAQRQMAMKKFGRLLPVENIHFDWGRESSNFIEMMSKVCPKMGIMIDRTETFIRTLQMVVIICPTSRDDRYSAIKKLCCCEQPVASQVINAKSVTTKIALQINCKLGGELWAVEIPMIIGIDVYHDVAKGKR--SIAGFVASMNKACTRYYSRCYFQGQNQEIDGLKICLISALRKYHPDKIVIYRDGVGDGQLRAVADYEVKQLHTCYNPNMALVIVQKRINTRLFNPPPGSVMDHSITRRDWYDFFLVSQHVRQGTVSPTHYIVVHDGTGLTVDRMQRLTYKMTHLYYNWPGTVRVPAPCQYAHKLAYLVGQSI
Mus_musculus_Arg1                       GTVGKPIKLLANYFYHYEVDIKPDKCPRYDGKKNIYTVTALPDRIFKVSIKWLAIVQALDVAMRLASMRYTPVGRSFFSPPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVRFTKEIKGEVTHCGQMKRKYRTFPLQLESGTVAQYFKQKYYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPD-RQEEISRLGRVLPAPILQYGW--DMRGFTDQLRKISKDAGMPIDSVEPMFRHLQLIIVILP-GKTPVYAEVKRVGDTLLGMATQCVQVKQTLSNLCLKINVKLGGIN-NILVPIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIEDLSYMVRELLIQFYPTRIIFYRDGVPEGQLPQILHYELLAIRDAYQPGITYIVVQKRHHTRLFNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHL
Mus_musculus_Arg2                       GTTGRTIKLQANFFYHYELDIKPEKCPRFDGRKNLYTAMPLPDRIFKVSIKWVSCVQALDVVMRLPSMRYTPVGRSFFTASPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGQMKRKYRTFPLQQESGTVAQYFKDRH-----VCNIVAGQRCIKKLTDNQTSTMIRATARSAPD-RQEEISKLGRVLQPPSILYGW--DMRNFTEQLRKISRDAGMPIDSVEPMFRHLQLVVVILP-GKTPVYAEVKRVGDTVLGMATQCVQMKQTLSNLCLKINVKLGGVN-NILLPIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIQDLAAMVRELLIQFYPTRIIFYRDGVSEGQFQQVLHHELLAIREAYQPGITFIVVQKRHHTRLFNIPAGTTVDTKITHPTEFDFYLCSHAGIQ---------------------------------VRR------------------------
Mus_musculus_Arg3                       GTMGKPIKLLANCFYLYEVDIKPDKCPRYDGKRSLYTANPLPDRPFKVSVKFVSRVHAVDVVLRLPSMKYTPVGRSFFSAPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSHRVKFTKEIKGEVTHCGTMRRKYRTFPLQLENGTVAQYFREKYYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPD-RQEEISRLGRVLPAPMLQYGW--DMRGFTDQLRKISKDAGMPIDSVEPMFRHLQLIIVILP-GKTPVYAEVKRVGDTLLGMATQCVQVKQTLSNLCLKINVKLGGIN-NILVPIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIQDLASMVRELLIQFYPTRIIFYRDGVSEGQFRQVLYYELLAIREAYQPGITYIVVQKRHHTRLFNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNFFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
Mus_musculus_Arg4                       GTVGKPIRLLANHFYHYDVDIKPEKRPRYDGKRNMYTAHPLPDQTFKVSVQWVSVVQALDVITRLPSMRYTPVGRSFFSPPPLGGGREVWFGFHQSMLNIDVSATAFYRAQPIPLTDSQRVKFTKEIRVESTHG-----KYSKYSLRILTE------HGKYYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPD-RQEEISRLGRVLPAPMLQYGW--DMRGFTDQLRKISKDAGMPIDSVEPMFKHLQLIVVILP-GKTPVYAEVKRVGDTLLGMATQCVQVKQTLSNLCLKMNAKLGGIN-NVLVPIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEVQDLTSMARELLIQFYPTRIIYYRGGVSEGQMKQVAWPELIAIRKAYRPGITYIVVQKRHHTRLFNVPAGTTVDSTVTHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHL
Mus_musculus_Piwi1                      GSSGIIVKLSTNHFYQYHIDYNPLMEARFDGTI-LFLPKRLQGEHVRITITLTNELQFYNIIFRLKIMNLQQIGRNYYNPSP-NHRLVIWPGFTTSMLCTDVSHKVL-RSETVTEEHKFQEQVSKELIGLIVLTKYNNKTYRTFKKA--DGSFLEYYRKQYMLIPELCYLTGLTDKMR--NDFNVMKDLAVHTRLTPEQRQREVGRLGRILQSEKIHQGWSKETRGLIQNLFKVTPAMGIQMDRTEAYLRALQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARAIATKIALQMNCKMGGELWRVDMPMIVGIDCYHDTTAGRR--SIAGFVASINEGMTRWFSRCVFQDRGQEVDGLKVCLQAALRAWSPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCYNPRLTVIVVKKRVNARFFNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSI
Mus_musculus_Piwi2                      GSKGTPQSLGLNLIYQYHVTFSPSVECKFDGSI-LYLPVKLQDAEISIKIQLTKILPFYNVVFRMKLLDMKLVGRNFYDPTQ-QHRLQIWPGYAASFLLADVSHKVI-RNDSVQNKEHFQDECSKLLVGSIVITRYNNRTYRSFVMS--DGTFLEYYSKNYLLLPELSFMTGIPEKMK--KDFRAMKDLTQQINLSPKQHHGALECLGRLLPMERINLRWVKEVTRLVNMLEKIAGPIGMRIDRIETYIRTIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRSVAQKILLQMNCKLGGELWGVDIPMVIGMDVYHDPSRGMR--SVVGFVASINLTLTKWYSRVVFQMPHQEVDSLKLCLVGSLKKYYPEKIVVYRDGVSDGQLKTVANYEIPQLQKCYHPKMVVFVVQKKISTNLYTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQIL
Nematostella_vectensis_Arg1             GSTGRTIALRANFFHHYDLSICPDKCPRFDGRKNLYTRDPLPDKKFKVTLKWVSQVQALDVVLRLPSMKYTPVGRSFFSPPPLERGREVWFGFHQSLLNIDVSATAFYKCQSVPLNDADRLRFTREIKGEITHCGPMKRKYRQFPLTLESGTVARYFQDKHYLPLEVCNIVPGQRCVKKLTDTQTSKMIRATARSAPD-REREIRGLGRVLPPPKLVYGW--DMRGFSNQLMKISVEQGMPIDEVERMFRRLQMILVVLP-GKTPVYAEVKRVGDTMLGVITQCIQGKQTLSNLCLKINAKLGGVN-NILAPIFLGADVTHPAAGDDKRPSVAAVVGSMDAHLSRYYASVRVQTHRQEIAELAAMVRELLVQFYPQRIVFYRDGVSEGQFRQVLVHELKAIREAYQPGISFIVVQKRHHTRLFNVPPGTTVDRGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNFSADELQALTYQLCHTYVRCTRAVSIPAPAYYAHLVAFRARYHM
Nematostella_vectensis_PiwiA            GSVGTKVGIVSNYFYQYNVSFSPEVDFKFDGMV-LFLPKKLPDEPVEITITLTNELHLFNIILKLKYIDMKQVGRNYYYIKP-KHGLELWPGYTTSLLGADVSHKVL-RKQTVQGRGNFHDEAAKKLVGEIVLTRYNNKTYRTFTTP--KGSFNDYYKKNYLLLPELCFLTGLSDDMR--ADFSVMKDLAVHTRLDPKARSKDLNKLGRILPGEKIFQKWSREIRGFQQTLARVCGPMGINTDRTDSYLKALQIVVTIVPSNRKDRYDSIKKLCCLEKGVPSQVVVSRSVCTKIGIQLNCKLGGEAWAVDIPMIVGIDTYHDSSQKGR--SVGGFVASLNKPCTRYYSRCTFQHSGQEVDGLKVCMTAALRQWQPDKIIVFRDGVGDGQLRAVIEHEVPQILSTYDPKFGFTVVKKRIQTRLFNPPPGTVVDKEVTRSEWFDFFIVSQSVRQGTVSPTHYNVIHDTTGLRPDHWQQLSYKLTHLYYNWPGTVRVPAPCQYAHKLAFLVGQSL
Nematostella_vectensis_PiwiB            ------IPLSCNYIFQYCVSYSPEIDNRFDSTM-LFLPRRLPGSPVTVTITFTRVVQVYNILFRMKILDMQQVGRYFYNPKP-QHNLVLWPGYITSMLLCDVHHRLL-RTDSCKVGDRFRDEVSKELIGSVVLTTYNNKTYRTFTFH--NGTFVEYYKKSYLLIPELCKLTGLNDAMR--KDFRVMKDIAAHTRVNPMQRQEAMKKFGRQFPPEKIIYGWGRDATRFVQTMNKCAPAMGIQVDRTETYLRSIQVVVVIFPTSRDDRYAAVKKLCCVESPVPSQVIISKSVVQKIALQINVKLGGELWALDIPMVCGIDVYHDKARGGR--SVGGLVCSMNRSLTRWYSDVCFQSPGQEIDGLKMLLIKGIRKWHPERIIVYRDGVGDGQLKVVAGYEVQQFEECYKPKLAVLVVQKRINTRIFNPGPGSVLDHTVTRKDWNDFFLVSQHVRQGTVTPTHYVVVYDTSNWKPDIMQKLSYKLTHLYYNWPGTVRVPAPCQYAHKLAYQVGQSI
Strongylocentrotus_purpuratus_Arg1      GIDGRPIALRANHFFHYEVNITPEKCPRFDGRTNMYCKDEIPDRIFKASIKFEGKVQALDVIMRLPSLRYTPVGRSFFSPPPLGGGREVWFGFHQSMLNIDVSATAFYSCQSVPLSDSHRVKFTKEIKGEITHCGNMKRKYRTFPWQLENGTVAKYFRERHYLPLEVCNIVAGQRCIKKLTDLQTSTMIKATARSAPD-REKEIKSLGRVLPAPKIQYGW--DMRGFTLQLQKISNEAGMPIDQVEAMFRHLQLIIVVLP-GKTPVYAEVKRVGDILLGIATQCVQVKQTLSNLCLKIKVKLGGVN-NILAPIFCGADVTHPPAGDDKKPSIAAVVGSMDGHPSRYCASVRIQTHRVEIQDLQAMVKELLVEFYPARIIMYRDGVSEGQFLQVLANEMNAIRDAYEPGITFIVVQKRHHTRLFNIPAGTTVDSGITHPLEYDFYLCSHAGIQGTSRPSHYHVLWDDNGFKADELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
Strongylocentrotus_purpuratus_Seawi     AIAGDKIALIANGFYQYRVDFEPEILNPLD-MDTMYSLFKLAGSNVNICVTHVATLHLYNVLFRLKMINMEQVGRNYYDPTK-QHGLQLWPGFVTSMLLSDISHKVL-RTQTVKARGRFKEEITKLMIGQIVLTKYNNKTYRTFETR--SGSYVDYFKKSYYLPPELCFLTGLSDDMR--ADFNVMKDMGQHTRVGPQDRCRTLSGFGRVLPPEKLFQRWSRDTRGFIKSLVRVANPMGMNVDRTETYTRSLQIVVVILPTNRKDRYDAIKKTCVVTHPCPSQVIVSRSVATKIAMQMNCKMGGDLWRVEIPMIIGIDSYHDSLTKGR--SVLGFVASMNKSQTSFFSSCAFQHAQGEGANLSTLMNNALKRYYPERIIIFRDGVGDSQVNLVVDYELKQIKDTTVHKLAVVVVKKRINNRFFNPPPGTVIDDVVTKPHLYDYFIISQSVRQGSVSPTSYNVVYDTTGLKPDHMQRLTYKLTHLYFNWPGTVRVPAPCMYAHKLAFLIGQSV
Tribolium_castaneum_Arg1                GREGRPIGLKANHFHHYDVSIQPDKCPRFDGRNNLYTRDPLPDRLFRVTIKWVAQVLALDVVMRLPSMSYTPVGRSFFSSPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGTMRRKYRSFPLQLDNGTVAKYFLDKYYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPD-REREINNLGRVLPPPKLQYGW--DMRGFTQQLQKISNDAGMPIDQVEPMFRYLQLVVVVLP-GKTPVYAEVKRVGDTVLGMATQCVQAKQTLSNLCLKINVKLGGIN-SILVPIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIQELSSMVRELLIMFYPHRIILYRDGVSEGQFLQLLQHELTAIREAYKPGITFIVVQKRHHTRLFNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDSHLDSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
Tribolium_castaneum_Arg2a               GVKGRPIKIESNHLYHYDVSITPD-TPKFDGRKNLYSPKKLPKRGFKVVVKLARTVQCLDIVLRAPSNSCISSGRCFFTPPRLGDGMEMYYGFYQSLLNVDVVHKAFPEALNVPLTDFVQKALEKFLKQEIPGQSGSRRIFR---FTLGDGTVEKYYQEVKLIPLEFCTVVSGQVVNRKMNENQTSVMIKKAATSTDV-RKDKIMQVGRVLQPPTLLYAWRADM--LADMIFRMASSNGMQIQNLRDIIDYFDLIIVVVP-NSGPQYSLVKQAAELNVGCLTQCIKERQIIANILLKINSKLNGTN-HILSSIIMGADVTHPGPDAKDVPSVAAVTASHDPNAFQYNICWRLQPPKVEIEDLCAITVEQLMFFYPETIVFFRDGVSEGQFAEVRRAEISAIHQAYEPRITFLVVQKRHHTRLFNVPAGTCVDTHITNPMMQDFYLVSHASIQGVAKPTKYCTLWDDNNMSNDDIEELTYYLCHMFTRCNRSVSYPAPTYYAHLAAARAKVYV
Tribolium_castaneum_Arg2b               GTKGRRIQIESNHLYHYDVAITPD-TPKFDGRKNLYSPKKLPKKEFKVEVKLARTVQCLDIVLRAPSNACIIAGRCFFTPPPLGDGMELYYGFYQSLLNVDVAHKAFPKASNVPLREFVQRDFEKFIKQEIPNQSSSKRIHR---FKLDDGTVERYYQEVKLLPLEFCTVVGGQAINRKMNENQTSAMIRKAATSTDV-RKDKIMQTARVLNPPSLLYAW--RADRLADMIFRMASSNGMQIQGLRDFIDYFDLIIVVVP-NSGPQYSFVKQAAELNVGCLTQCIKERQTVGNILLKINSKMNGTN-HRLSPMIMGADVTHPSPDARDIPSVAAVTASHDPNAFQYNICWRLQPPKVEIEDLCNITVEQLKFFYPESIVFFRDGVSEGQFKQVQRAEIAAIQKAYEPKITFLVVQKRHHTRLFNVPAGTCVDTHITNPRMQDFYLVSHASIQGVAKPTKYCTLWDDNNMNNDDIEELTYHLCHMFTRCNRSVSYPAPTYYAHLAAARAKVYI
Tribolium_castaneum_Piwi                GTIGTPINLIANYLYQYRVDMAPDVDNTFDGTV-LYTTQRIN--NVRITIRLVGDLQLFNIIIRLKLMGLQQVGRNYFMPDS-EHKIQLWPGYFTSLLNVDLQFK-FMRTDTVCQGANARKEFQSKIIGSVVLTHYNNKTYKTFKLK--DGTFKDYFKKKYYLVPELCLMTGLTDRQR--ENFNLMKMLATHTRIGVEGRIKKLMEFGRVLPQETVVGGWTKELRSFIQLLAKTAGGMRWSLDRSGSYIEQITMILVILPNNSTERYSAIKKKCYVDRGIPTQMFVARSIATKVAIQMNCKIGGAPWCVPIPMVVGYDVCRDTVNKKK--SFAGIVGSLDKNISRFYNICCEHKMEEESDNFAAAVVLLCKQYKPERILIYRDGVGEGQLPFVVEHEVANIKRKGEVKMAFVVVSKRINTRIFNPPPGTVVDDVITLPERYDFYIVSQCVRQGTVAPTSYNVIEDSMGLPPEKLQYLTYKLTHMYYNWSGTVRVPAPCQYAHKLAFMVSQYI
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