#NEXUS [!This data set was downloaded from TreeBASE, a relational database of phylogenetic knowledge. TreeBASE has been supported by the NSF, Harvard University, Yale University, SDSC and UC Davis. Please do not remove this acknowledgment from the Nexus file. Generated on September 18, 2025; 21:25 GMT TreeBASE (cc) 1994-2008 Study reference: Cho S., Valles Y., & Weisblat D. 2013. Differential expression of conserved germ line markers, and delayed segregation of male and female primordial germ cells, in a hermaphrodite, the leech Helobdella. Molecular Biology and Evolution, . TreeBASE Study URI: http://purl.org/phylo/treebase/phylows/study/TB2:S14911] BEGIN TAXA; TITLE Taxa; DIMENSIONS NTAX=26; TAXLABELS Bombyx_mori_Vasa Capitella_teleta_PL10 Capitella_teleta_Vasa Ciona_savignyi_Vasa Danio_rerio_PL10 Danio_rerio_Vasa Helobdella_robusta_P68 Helobdella_robusta_PL10a Helobdella_robusta_PL10b Helobdella_robusta_PL10c Helobdella_robusta_PL10d Helobdella_robusta_Vasa1 Helobdella_robusta_Vasa2 Lottia_gigantea_PL10 Lottia_gigantea_Vasa Mus_musculus_PL10 Mus_musculus_Vasa Nematostella_vectensis_PL10 Nematostella_vectensis_Vasa2 Platynereis_dumerilii_PL10a Platynereis_dumerilii_PL10b Platynereis_dumerilii_Vasa Strongylocentrotus_purpuratus_P68 Tribolium_castaneum_P68 Tribolium_castaneum_PL10 Tribolium_castaneum_Vasa ; END; BEGIN CHARACTERS; [! TreeBASE Matrix URI: http://purl.org/phylo/treebase/phylows/matrix/TB2:M19426] TITLE 'Vasa-PL10'; LINK TAXA = Taxa; DIMENSIONS NCHAR=370; FORMAT DATATYPE=Protein SYMBOLS= "A C D E F G H I K L M N P Q R S T V W Y" MISSING=? GAP= -; MATRIX [ 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370] [ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .] Bombyx_mori_Vasa GGGGGETEIFSGINFDKFDHIAVKVSGENPPNVLKAGYRKPTPIQKNAIPIIMSGRDLMGCAQTGSGKTAAFLVPIINMLLQDPPQVIIVSPTRELTLQIFNEARKFSYGSVLKVAVAYGGTAVRHQGDNIGCHILVATPGRLHDFVERNRVSFGSVRFVVLDEADRMLDMGFMPSIEKMMLHPTMVETTKRQTLMFSATFPEDIQHLAGRFLLFVAVGIVGVEQIFIEVTKYEKRNSLKQLIILVFVETKRNADFIAAMLSEQQLLTSSIHGDRMQREREEALFKSGKHCILVATAVAARGLDIKNVDIVVNYDLPKSIDEYVHRIGRTGRVGNRGKAVSFYLSKILRQADQSVPDFLK---YGGSDVR Capitella_teleta_PL10 GGQGGEHELFTGINFDKYESIPVEATGEGAPNIELCKYTCPTPVQKYAIPVIIGKRDLMACAQTGSGKTAAFLLPVLSRLYQMGPLALVLAPTRELATQIYDEARKFAYRSHVRPCVVYGGADIGAQMRDLGCHLLVATPGRLVDMMERGKVGVERIRFLVLDEADRMLDMGFEPQIRRIVEKDNMPVTGDRQTLMFSATFPKEIQMLARDFLIFLAVGRVGITQKVVWVEDNDKRSFLLDLLTLVFVETKKGADSLEYFLYTEGYPAGSIHGDRSQREREDALFRSGKTPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFLVSLLMEAHQEVPGWLESMCFGARDYR Capitella_teleta_Vasa AGSSGEEAIFMGINFDKYDDIPVECTGNNAPNVRKAKYDRPTPIQKWAIPAIIKGRDIMACAQTGSGKTAAFLLPTLTNMLNSGPLAVVIGPTRELVSQTQGEARKFSYDSMIKSVVVYGGTSVQYQLRQVGCNVVVGTPGRLLDFVGKGKISLKKVKHLILDEADRMLDMGFKGDIERLVTQFGMPPKEERQTLMFSATFPEPIQQLAQEFLLFITVGRVGIEQRIFEVAQHDKKSKLEEILTLVFVEKKRQADYLASYLSQSEFPTTSIHGDRQQREREEALFKSGKCPILVATSVAARGLDIPGVKHVINFDFPSDIDEYVHRIGRTGRCGNMGKASSFFLVKKLEDAQQEVPSFLEAAAFGGKDTR Ciona_savignyi_Vasa GGGGGEVEMFRGINFGKYDAIPVEVSGVNAPNVKRANYERPTPVQKYSIPIINADRDLMACAQTGSGKTAAFLLPVLTKLITNGPRAIVVGPTRELIYQIFLEARKFSRGTVVRPVVAYGGTSMNHQIRDLGCHILIATPGRLMDFINRGLVGLDHVEFVILDEADRMLDMGFETEIRKLASSPGMPSKSDRHTLMFSATFPDEIQRLAHDFLLFLTVGRVGVTQSIIQVDQDDKRAKLLELITLVFVETKRGADFLACMLSQEGCPTTSIHGDRLQQEREQALFKSAVCPILIATSVAARGLDIPKVEHVINYDMPKEIDEYVHRIGRTGRCGNLGRATTFYLVKILSEAQQEVPGWLEECAFGGRDFR Danio_rerio_PL10 GGFGGEHELFTGINFEKYDDIPVEATGHNGPNINLSRYTRPTPVQKHAIPIIKSKRDLMACAQTGSGKTAAFLLPVLSQIYTDGPISLVLAPTRELALQIYDEARKFSYRSHVRPCVVYGGADIGQQIRDLGCHLLVATPGRLLDMMERGKIGLDYCNYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGLRQTMMFSATFPKEIQILARDFLIFLAVGRVGITQKVVWVEENDKRSFLLDLLTLVFVETKKGADALEDFLYREGYACTSIHGDRSQRDREEALFRSGRCPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFLLDILVEAKQEVPSWLESLAFGARDYR Danio_rerio_Vasa GGFRGESSIFTGINFDKYDDILVDVSGSNPPNVSKSGYVKPTPVQKHGIPIISAGRDLMACAQTGSGKTAAFLLPILQRFMTDGPEAIIVAPTRELINQIYLEARKFAYGTCVRPVVVYGGINTGYTIREVGCNVLCATPGRLHDLIGRGKIGLSKVRYLVLDEADRMLDMGFEPEMRKLVASPGMPSKEERQTLMFSATYPEDIQRMAADFLIFLAVGVVGVEQTIVQVDQYSKRDQLLELLTMVFVETKRSADFIATFLCQEKISTTSIHGDREQREREKALFRLGQCPVLVATSVAARGLDIEQVQHVVNFDMPSSIDEYVHRIGRTGRCGNTGRAVSFFLVKVLSGAQQVVPKWLEEVAFASTDSR Helobdella_robusta_P68 -----ENPITDDVQF-FYNENQITISGTQCSVLMQQNWTKPTPIQSQGWPLALSGRDLVGIAQTGSGKTLSYILPAVVHI----PICLVLVPTRELAQQVAQVAQMFGSSSRVRNACIYGGAPKGPQIRDIGAEICIATPGRLIDILEMRKVNLKRCTYLVLDEADRMLDMGFEPQIKKIISQ----VRPDRQTLMWSATWPKEVERLAESFLVQINIGATQILQIVQVCQEDEKDRKLDRLLTIIFVETKKRADELTRKMKRVGWISACIHGDKSQPERDWVLFRENKNPILVATDVAARGLDVDDIKYVINYDFPNSSEDYVHRIGRTGRASKVGTAFTFFLISVLQEANQQVDPALFAMQYGGPTKR Helobdella_robusta_PL10a GGAGGENELFTGINFDKYEDIPVEATGRDCPNISLCKYDRPTPVQKYSIPVVLNKRDLMACAQTGSGKTAAFLVPILSQIYSGGPIALVLAPTRELASQIYDEARKFAYRSHIRPCVVYGGADIGEQIRDLGCHLLVATPGRLVDMMERGRIAVDNVRFLVLDEADRMLDMGFEPQIRRIVEKDSMPTTGERQTLMFSATFPKEIQMLAKDFLIFLAVGRVGITQKVVWVEEEEKRSSLLDLITLVFVETKKGADALDYFLYKEGFPSTSIHGDRTQQEREDALFRDGRTPILVATAVAARGLDIPHVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFLLDILVEAHQEVPPWLESLAFLKTSYL Helobdella_robusta_PL10b VGLGYEHELFTGINFSKYEDIPVEATGADCPNIKLSQYERPTPIQKYAIPIIVKKRDIMACAQTGSGKTAAFLVPILSQIYANGPIALVLAPTRELASQIHDEARKFSYRSHVRACIVYGGADINAQMQDLGCHLLVATPGRLVDMIERGKIGMDRVQYLVLDEADRMLDMGFEPQIRRIVEKDTMPATGVRHTLMFSATFPREIQILARDFLIFLAVGRVGITQKVLWVEEYEKRVVLLELLILIFVETKQGADQLHAFLSMENFPTNSIHGDRSQRQREEALFKTGKTPILVATAVAARGLDISNVKHVINFDLPGSIEEYVHRIGRTGRVGNLGLATSFFLLELLSEAHQEVPIWLNDMAFASRDYR Helobdella_robusta_PL10c -----EHELFSGINFAKYEDIPVETSGNNCPNIKLCKYTHPTPIQKYAVPVILAKRDIMGCAQTGSGKTAAYLLPLFSNIIETGPCALVLTPTRELACQVFDEAKKFLYRSRVQPCVIYGGADAGTQIREIGCHVLIATPGRLIDLLERGLVGLDAIKYLVLDEADRMLDMGFEPQIRRIVVEDDMPQTGRRQTLMFSATFPKEIQMLANEFLIFLAVGRVGITQKVLWVDEEEKRAFLKELLTLIFVETKKGADSLEFYLYKEGYMVTSIHGDRIQKEREEALFKCGKRPILVATAVAARGLDIANVKHVINYDLPTDIEEYVHRIGRTGRVGNLGLATSFFLFEILAESKQEIPEWLENLAYNGVPKK Helobdella_robusta_PL10d -------------------------------NIKLSQYERPTLMQKYAIPIIVKKRDIMACAQTGSGKTAAFLVPILGQM----PIALILAPTRELASKIHDEARRVRYCPHVRVCIECGGADINAQMQDLGCHLLVATPGRLVDMIERGKIGMDRVQYLVLDEADRMLDMVFEPQIRRIVEKDTLLATGVRHILMFSATFPREIQILARDFLIFLA-------------------GCPLELLILIFVETKQGADQLHAFLSMENFSTNSIHGDRSQRQREEALFKTGKTPILVATAVAARGLDISNVKLIYQFNVEVASAAYMFLVGTPGRLQDLGSIQHLIIHKLIEESNMTAKESRQTLMFLKPDYL Helobdella_robusta_Vasa1 -----ENQMFKGINFDKYEDIPVDCTGQNPPNVVKAKYDRPTPIQKWSIPTILLGRDLMACAQTGSGKTAGFLLPVLTGMLERGPQAIVIGPTRELVAQIYKEAYKFSLNTRIRPVVVYGGVSVSHQLHEIGAHIVIATPGRLIDFINREKIGLSKVKYLVLDEADRMLDMGFKDDITKICCRMGMPDKFNRQTLMFSATFPSEIQQIAHQLLIFITVGMVGIIQNIELVENKEKQEKLLSILTLIFVEKKKMTDFLALVLCQHNLPATSIHGDREQREREEALFKSGKTPILVATSVASRGLDIPGVNHVINYDLPASIDEYVHRIGRTGRCGNIGQATSFFLVKILSEASQEVPHWLEE--FEGQSEE Helobdella_robusta_Vasa2 -----ESYLFKGKNFDRLCTYSVELTGNNAPNIKKAGYSLPTPIQQWSIPAILHGRDLMACAQTGSGKTAAFLIPVLSLLLADNPLVVILTPTRELALQTHKEIRKFAFQTVIKSCCVYGGVSVNYQLNSMGVHVIVGTVGRVSDFTRNGKIDLSHVKYLILDEADRMLNMGFHDEVVALTEKCGGMRSEQRRTMLYSATFPVEIQKLAFDILLFVTVGLVGIEQSILKVNKLEKKEKLKEILVIVFVKTKLLADSLCIQFSEEEIPMTTIHGDLSQKERELVIFREDRASVLCATSVAARGLDIPGVKHVINYDLPDNIDEYVHRIGRTARCGNTGKAVSFFLYTEKFTSRRN---KLYQIG------- Lottia_gigantea_PL10 ADSGDEHELFTGINFDKYEDIPVEASGENPPNIVLSKYPKPTPVQKYAIPIVLNKRDLMACAQTGSGKTAAFLVPILNLLYETGPSALVLAPTRELASQIYDEARKFAYRSRVRPCVVYGGADIGAQMRDLGCHLLVATPGRLVDLMERGKVELQHCRYLMLDEADRMLDMGFEPQIRRIVEKDSMPPSGKRQTLMFSATFPKEIQILARDFLIFLAVGRVGITQKVVWVEESEKRSFLLDLITLVFVETKKGADALEVFLTREGFPATSIHGDRSQREREDALFRSGDRPILVATAVAARGLDISNVRHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFILLDLLMEANQEVPSWLEAVAFGARDYR Lottia_gigantea_Vasa GSSGGEDEIFKGINFNKYDDIPVEVSGRSPVNVRKAKYDRPTPVQKYAIPIIMAGRDLMACAQTGSGKTAAFVLPTITAMMNDGPQAIVVAPTRELAIQIHLDARKFAHGTDLRSVVLYGGTSVGYQMKQVGAHFVVGTPGRLLDTINKGKISLAKIKFLILDEADRMLDMGFEPDIRKLVETMGMPAKTERQTLMFSATFPEEIQKLAADFLLFVTVGRVGVEQMFYEIDRLQKRDKLCSILTLVFVEQKRNADFLASYLSQSVFPTTSIHGDRLQREREEALFKTGKAPILVATSVAARGLDIPGVKHVVNYDLPASIDEYVHRIGRTGRCGNLGKATSFYLVRILKEANQGVPSWLESYAFGGKDIR Mus_musculus_PL10 GGRSGEQELFTGINFEKYDDIPVEATGNNCPNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYTDGPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLIFLAVGRVGITQKVVWVEEADKRSFLLDLLILVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFLLDLLVEAKQEVPSWLENMAFGARDYR Mus_musculus_Vasa GGYKGEDSIFTGINFDKYDTILVEVSGHDAPNIAKAGYTKLTPVQKYSIPIVLAGRDLMACAQTGSGKTAAFLLPILAHMMRDGPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQFGHSVRQIGCNILCATPGRLMDIIGKEKIGLKQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLLFSATFPEEIQRLAGDFLLFVAVGQVGVQQTILQVGQYSKREKLVEILTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFLVKVLSDAQQDVPAWLEEIAFASVDTR Nematostella_vectensis_PL10 ----------------------------------------------------------------------AFLIPILSRIYMEGPICLVLAPTRELAVQIFDEARKFSYRSLCRPCVVYGGADIGSQLRELNCHLLVATPGRLVDMMDRGRVGLDSIRFLVLDEADRMLDMGFEPQIRRIVDQDSMPKTGIRQTLMFSATFPKEIQMLARDFLIFLAVGKVGITQKVVWVDEFDKRSFLLDLLTLVFVETKKGADALEMFLAKDGYYCTSIHGDRSQREREEALFRCGDTPILVATAVAARGLDIPNVKHVINFDLPTDIEE------------------------------------------------ Nematostella_vectensis_Vasa2 ----------------------------------------------------------------------AFLLPVMTSMMNAGPQAMCIAPTRELANQIYLEARKFAHGTMLRPVVCYGGVSVSHQLRQLGCNLLVGTPGRLTDFIEKGKVSLKGLQFLILDEADRMLDMGFEPAIRRIVESMGMPDKSERQTLMFSATFPEEIQRLAGDFLLFLTVGRVGIEQTVIEVTDNEKRDRLTQILTLVFVESKRSADFLAIFLSGEGFPTTSIHGDRLQQEREEALFRKGRCPVLIATNVAARGLDIDDVKHVINFDLPSDID------------------------------------------------- Platynereis_dumerilii_PL10a GGGGGEKELFTGINFDKYEDIPVEATGEDCPNIVLSKYTKPTPVQKHAMPIIKKKRDLMACAQTGSGKTASFLVPVLSQMFLDGPIALVLAPTRELASQIYEEARKFAYRSHVRPCVVYGGADIGAQMRDLGCHLLVATPGRLVDMLERGKIGLDHIRFVVLDEADRMLDMGFEPQIRRIVEKDTMPPTGDRRTLMFSATFPKEIQILARDFLIFLAVGRVGITQKVVWVEEPDKRSFLLDLLTLVFVETKKGADALDNFLYNEGYPSACIHGDRSQKEREEALFRSGKTPVLVATAVAARGLDIPNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFMVDLLIEAKQEVPPWIESIAFGSRDYR Platynereis_dumerilii_PL10b GGGGGEKELFTGINFDKYEDIPVEATGEDCPNIVLSKYTKPTPVQKHAMPIIKKKRDLMACAQTGSGKTASFLVPVLSQMFLDGPIALVLAPTRELASQIYEEARKFAYRSHVRPCVVYGGADIGAQMRDLGCHLLVATPGRLVDMLERGKIGLDHIRFVVLDEADRMLDMGFEPQIRRIVEKDTMPPTGDRRTLMFSATFPKEIQILARDFLIFLAVGRVGITQKVVWVEEPDKRSFLLDLLTLVFVETKKGADALDNFLYNEGYPSACIHGDRSQKEREEALFRSGKTPVLVATAVAARGLDIPNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFMVDLLIEAKQEVPPWIESIAFGSRDYR Platynereis_dumerilii_Vasa GGSGGEEEMFAGINFDKYESIPVEVSGTNAPNVRKAKYDRPTPIQKWAIPIVLSGKDLMGCAQTGSGKTAAFLLPVLTGIIKNDPAAIIVGPTRELVNQIYLEARKFASSTCVRPVVVYGGTSVGYQARELGAHVVVGTPGRLLDFIGKGKINLSKVKYLILDEADRMLDMGFEPEIRKLVTTFDMPEKGQRQTLMFSATFAAEIQQLAKEFLVFVTVGRVGITQEVHQVTKYEKREKLVEILTLVFLETKRSADFLAAYLSQEQYPATSIHGDRLQREREEALFKTGRAPILIATSVAARGLDIPGVKHVINYDLPSGIDEYVHRIGRTGRCGNLGKATSFFLVKTLGDAQQVVPPWLEEIAFGAKDTR Strongylocentrotus_purpuratus_P68 GSMGGEHPDVTEIQ-SYCTDKQITVRGKEPMELSKSGFSHPTPIQAQGWPVALSGRDLVGIAATGSGKTLSYLLPSIVHI----PIALVLAPTRELAQQVQQVAFAFGRSSKIKSTCVYGGAPKGQQIRDLGVEICIATPGRLIDFLENNKTNLRRCTYVVLDEADRMLDMGFEPQIRKIMEQ----IRPDRQVQMWSATWPKDVRNLAEDFIIMVNIGSLTILQIIDVCEDSEKDKKLIQLLTLVFCETKRRTDDLVRRMRRDGWPAMCLHGDKSQPERDWVLFRDGRAPILVATDVASRGLDVTDIKFVINYDYPNSSEDYVHRIGRTARSTRTGTAYTFFLINVLQEAKQVINPKLISLAFGGGGMG Tribolium_castaneum_P68 SSSGNNSQSTTNNNNNNYSNNSEHESSTNSGDFAV---HRPSQARPPSKPSTLSKRNV------GCGTVAMKASPLI-------PWHVALYH---------------------------------------GAQLLYTCGGTLIS--ENHVLTAAHC--VAKPQTNRPIDT---KDLSVYLGKYHLKKFGD-------GTQDRDVTDI---FIYFNDIAVLKLNNYVRPCCLWEDGTDIEYVLGWGFDEKRQISDTLMQAQMPVVSTVNCIYSNRSQFTFEKTYFRNEDAGTTVCNGDSGGGMVFPKAGTSGH-----------NTVWQIRGIVSVGVAL---RHYIVFTDVAKHLDWIKQVM------- Tribolium_castaneum_PL10 GGGGGEQELFTGINFSKYEDIPVEATGDKVPNINLARYDTPTPVQKYAIPIIVGKRDVMACAQTGSGKTAAFLVPILNQMYEHGPLGLVLAPTRELATQIYDESKKFAYRSRVRPCVVYGGAHIGDQMRDLGCHLLVATPGRLLDMIDRGRIGLDYCRYLVLDEADRMLDMGFELQIRRIVEKETMPKTGERQTLMFSATFPSPIQMLARDFLIFLAVGRVGITQKVVWVEEHDKRSFLLDLLTLVFVETKKGADSLEEFLHFEGYPVTSIHGDRSQREREDALFRSGNTPILVATAVAARGLDIPHVKHVINFDLPSDIEEYVHRIGRTGRMGNLGLATSFFLLDLLIEAKQEYPSWLEGVAFGGRDYR Tribolium_castaneum_Vasa GGGRGE-DLFTGVNFMKLDEIEVKVTGNDAPNVKKSGYTKPTAIQKYAIPVILSGRDLMSCAQTGSGKTAAFMLPIIHNLLSDKPVVVIMSPTRELAIQIADQGKKFAYNSTVKVAVIYGGTSTNHQRGRIGCHILVATPGRLKDFVNRGNVSFNSLKYFVLDEADRMLDMGFLGDVEEMLSHQSMPATGERQTLMFSATFPEEVQQLAGKFLIFIAVGIVGVEQKFFQVSKFDKRSKLVSILTLIFVETKRNADFLATFLSEQNIQSTSIHGDRYQSEREKALFKTGHRKVLVATGVAARGLDIKDVQHVINYDLPKSIDEYVHRIGRTGRVGNKGKATSFFLAKILSQAKQEIPEWLGSKSFGGRDIR ; END;