#NEXUS

BEGIN TAXA;
	TITLE Taxa;
	DIMENSIONS NTAX=6;
	TAXLABELS
		Volvox_ovalis_sp._nov. Volvox_spermatosphaera Volvox_tertius_NIES544 Volvox_tertius_UTEX132 Volvox_dissipatrix Volvox_africanus 
	;

END;


BEGIN CHARACTERS;
	TITLE  Character_Matrix;
	DIMENSIONS  NCHAR=248;
	FORMAT DATATYPE = DNA GAP = - MISSING = ?;
	MATRIX
	Volvox_ovalis_sp._nov.  ACCCTCCCACAT-ACACATGGGTGGTGTGTGGACATGGCTGTCTCGGTGCGTCATATGCTGCATGAACCTCCTGCGTTGCAATGGCATTCATGAAGCATATGACCTGTGCTGGGTTGGCTGAAGAACAGCTGCTGTACCATGGACCAAACAACAGGTCTCTACTAGGTAGGCAATCCCTTCTGCA--CTTTAGTAGAAGATTTGGGTGCTGTGTGCGGCTCAAAGCAGGAGCTTCCTCAGAAGCACTA
	Volvox_spermatosphaera  ACCCTCCC-CACTTT-CATGTGTGATGTGTGGATCTGGCTGTCTCAGTACATCATG--CTACGTGAACGTCTTGTATAGTGCAACAACATTTGCGGTACATGAATGGCGCTGGGTTGGCTGAAGTACAGCTGCTGTACCATGGACCATACAACAGGTCTCTACTAGGTAGGCAATAACTTCTACA--CTTTAGTAGAAGACTTGGGTGCTGTGTACGGCACAAAGCAGGAACTTCTTTTGAAGCATGT
	Volvox_tertius_NIES544  ACCCCCTCACACCTTACATATGTGATGTGTGGATCTGGCTGTCTCAGT-TGTCATGTTTGATGTAATTGTGTTACGTATTGTGATGCAATTCATTAA-CGTGAGATGCGCTGGGTTGGCTGAAGTGCAGCTGCTGTACCATGGACCATACAACAGGTCTCTACTAGGTAGGCAATCACTTCTGAACGCTTTAGTAGACGACTTGGGTGCTGTGTACAGCACGAAGCAGGAACTTGTTTGCAAGCATGC
	Volvox_tertius_UTEX132  ACCCCCTCACACCTTACGTATGTGATGTGTGGATCTGGCTGTCTCAGT-TATCATGTTTGATGTAATTGCATTATGTATTGTGATGCAATTCATAAAATGTGAGATACGCTGGGTTGGCTGAAGTGCAGCTGCTGTACCATGGACCATACAACAGGTCTCTACTAGGTAGGCAATCACTTCTCAATGCTTTAGTAGACGACTTGGGTGCTGTGTACCGCACGAAGCAGGAACTTGTTTGCAAGCATAT
	Volvox_dissipatrix      ACCCCCTCCAATCTCTCTCA-GAGA-GAGAGGATCTGGCTGTCTCTAG-TTCTATCCATATCTTGAAGAGGATGAACATCTCTTAAATGTCCTCTTCTTGGGATGTGTGAAGAGTCAGCTGAAATGAAGCTGCTAAACCATGGACCACCACAGATGTGTTTG-TTTGCTGAAAATACTTTATACATCTTGTGTATGGTTATATACATACAGTGTTTGGCACAAGACAGGAAAATGTGATAATCTATAT
	Volvox_africanus        GTCCC-TCACGAAACGGTCTGCCGTCACGTGGATCTGGCTATCCCAGCCCCGTAGCCCCTGC--------------GAT----------------GTGGGGGGTGCACGCTGGGTTCGCTAAAGTGATGATGCTAGACTGTGGACCGCTGAACGAGTCTGTG-GATGTGTACATGTACTTCTCTATCTGATAGTGGATGGCTTGGGCCCTGTGTTTGGCACAGGGGAGGCGTATGTATGAACGTGCAT

;



END;

BEGIN PAUP;
set criterion = likelihood;
Lset Base = ( 0.2417 0.2139 0.2556 )  Nst = 6 Rmat = ( 3.3478 8.6020 3.1358 1.1690 8.6020 )  Rates = gamma Shape = 0.7590 Pinvar = 0;
END;


BEGIN TREES;
	Title 'ITS2-248 TB';
	LINK Taxa = Taxa;
	TRANSLATE
		1 Volvox_ovalis_sp._nov.,
		2 Volvox_spermatosphaera,
		3 Volvox_tertius_NIES544,
		4 Volvox_tertius_UTEX132,
		5 Volvox_dissipatrix,
		6 Volvox_africanus;
	TREE PAUP_1 = (5:0.6843985,(6:1.325412,((1:0.326604,2:9.55E-4):0.157495,(3:0.019338,4:0.039605):0.095471):0.289621):0.6843985)[% ] [% ] [%  setBetweenBits = selected ];

END;