#NEXUS

BEGIN TAXA;
	TITLE Taxa;
	DIMENSIONS NTAX=28;
	TAXLABELS
		Tribolium_castaneum_Piwi Bombyx_mori_Piwi Helobdella_robusta_Piwi1 Mus_musculus_Piwi2 Strongylocentrotus_purpuratus_seawi Helobdella_robusta_Piwi2 Capitella_teleta_Piwi2 Nematostella_vectensis_PiwiB Lottia_gigantea_Piwi2 Capitella_teleta_Piwi1 Mus_musculus_Piwi1 Nematostella_vectensis_PiwiA Lottia_gigantea_Piwi1 Bombyx_mori_Arg2 Tribolium_castaneum_Arg2a Tribolium_castaneum_Arg2b Nematostella_vectensis_Arg1 Helobdella_robusta_Arg2 Strongylocentrotus_purpuratus_Arg1 Bombyx_mori_Arg1 Tribolium_castaneum_Arg1 Lottia_gigantea_Arg Capitella_teleta_Arg1 Helobdella_robusta_Arg1 Mus_musculus_Arg4 Mus_musculus_Arg2 Mus_musculus_Arg1 Mus_musculus_Arg3 
	;
END;

BEGIN CHARACTERS;
	TITLE  'Piwi-Argonaute';
	DIMENSIONS  NCHAR=512;
	FORMAT DATATYPE = Protein GAP = - MISSING = ?;
	MATRIX
	Tribolium_castaneum_Piwi             GTIGTPINLIANYLYQYRVDMAPDVDNTFDGTV-LYTTQRIN--NVRITIRLVGDLQLFNIIIRLKLMGLQQVGRNYFMPDS-EHKIQLWPGYFTSLLNVDLQFK-FMRTDTVCQGANARKEFQSKIIGSVVLTHYNNKTYKTFKLK--DGTFKDYFKKKYYLVPELCLMTGLTDRQR--ENFNLMKMLATHTRIGVEGRIKKLMEFGRVLPQETVVGGWTKELRSFIQLLAKTAGGMRWSLDRSGSYIEQITMILVILPNNSTERYSAIKKKCYVDRGIPTQMFVARSIATKVAIQMNCKIGGAPWCVPIPMVVGYDVCRDTVNKKK--SFAGIVGSLDKNISRFYNICCEHKMEEESDNFAAAVVLLCKQYKPERILIYRDGVGEGQLPFVVEHEVANIKRKGEVKMAFVVVSKRINTRIFNPPPGTVVDDVITLPERYDFYIVSQCVRQGTVAPTSYNVIEDSMGLPPEKLQYLTYKLTHMYYNWSGTVRVPAPCQYAHKLAFMVSQYI
	Bombyx_mori_Piwi                     GTSGTPLDLLANYFYQYHVDISPEEDSTFDGTV-LYTVNRLHNERMRILIKLTCEVQIFNIIIRFNLLKLQLMGRDYFDPEP-EFKLQIWPGYKTTLLVTEIAHKVL-RMDTVTKGNNYKKIFLEDVVGKIVMTDYNKRTYRTFKMRDEN-TYIEYYYKKYYLVPELCRQTGLSDEMR--ANFKLMRSLDVHTKIGPDKRIEKLNNFGRQLPPENIIQAWTRDMRSFIDLIIKTGGGVGFRMIEYANMCEEVALILCVLARNYADRYEAIKKKCTVDRAVPTQVVCARSIATKVAIQINCKLGGSPWTVDIPMVVGYDVCHDTRSKEK--SFGAFVATLDKQMTQYYSIVNAHTSGEESSHMGFNIASAVKKFRPARIFIYRDGVGDGQIPYVHSHEVAEIKKKVEIKLAFIIVSKRINTRIFNPRPGTVIDDVVTLPERYDFYLVSQNVREGTIAPTSYNVIEDTTGLNPDRIQRLTYKLTHLYFNCSSQVRVPSVCQYAHKLAFLAANSL
	Helobdella_robusta_Piwi1             GLSGTRLTICTNLFFQYHVDFVPDIPNIFDGSI-LFTSHRIDGEDIKISIRYTNAVQIYNLIFKLREIGLQIVGRNYFNPQR-ELGLKVWPGYFTSNLMVDICHKVL-GQETVLRPRSYVEDSKRDVVGKIVLTRYNNRTYRTFPMR--DGSFKEYYRTRHCLIPELCIVTGISDEIK--SNFHSMRELSTHTRLGPHERSGKIEQLARTLEAEQIFLGWSRDMRAFFTKLVQAGQMMGVKIESNRSYIQTLQLVMCVLESEKKDKYDVIKKTLCINVPIPSQVMLMKSVATKVVIQVNCKVGGEGWAVSIPMVIGIDTYHDSSKKYR--SVVGIVGSTNSSFTKYHTTWAFQEKGEESSCLCAAIASLVKAFTPGRIFVYRDGVGDGQLGMVYDHELLQIQEGYKPKITLFVVKKRVNNRFFNPPPGTVIDTKATRPHWYDFFLISQSVNQGTVTPTHYNILHDDSGLKPDHLQRLTYKLCHLYFNWQGTVRVPAPCQYAHKVAFLIGQSL
	Mus_musculus_Piwi2                   GSKGTPQSLGLNLIYQYHVTFSPSVECKFDGSI-LYLPVKLQDAEISIKIQLTKILPFYNVVFRMKLLDMKLVGRNFYDPTQ-QHRLQIWPGYAASFLLADVSHKVI-RNDSVQNKEHFQDECSKLLVGSIVITRYNNRTYRSFVMS--DGTFLEYYSKNYLLLPELSFMTGIPEKMK--KDFRAMKDLTQQINLSPKQHHGALECLGRLLPMERINLRWVKEVTRLVNMLEKIAGPIGMRIDRIETYIRTIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRSVAQKILLQMNCKLGGELWGVDIPMVIGMDVYHDPSRGMR--SVVGFVASINLTLTKWYSRVVFQMPHQEVDSLKLCLVGSLKKYYPEKIVVYRDGVSDGQLKTVANYEIPQLQKCYHPKMVVFVVQKKISTNLYTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQIL
	Strongylocentrotus_purpuratus_seawi  AIAGDKIALIANGFYQYRVDFEPEILNPLD-MDTMYSLFKLAGSNVNICVTHVATLHLYNVLFRLKMINMEQVGRNYYDPTK-QHGLQLWPGFVTSMLLSDISHKVL-RTQTVKARGRFKEEITKLMIGQIVLTKYNNKTYRTFETR--SGSYVDYFKKSYYLPPELCFLTGLSDDMR--ADFNVMKDMGQHTRVGPQDRCRTLSGFGRVLPPEKLFQRWSRDTRGFIKSLVRVANPMGMNVDRTETYTRSLQIVVVILPTNRKDRYDAIKKTCVVTHPCPSQVIVSRSVATKIAMQMNCKMGGDLWRVEIPMIIGIDSYHDSLTKGR--SVLGFVASMNKSQTSFFSSCAFQHAQGEGANLSTLMNNALKRYYPERIIIFRDGVGDSQVNLVVDYELKQIKDTTVHKLAVVVVKKRINNRFFNPPPGTVIDDVVTKPHLYDYFIISQSVRQGSVSPTSYNVVYDTTGLKPDHMQRLTYKLTHLYFNWPGTVRVPAPCMYAHKLAFLIGQSV
	Helobdella_robusta_Piwi2             GSTGKKVPLCANYIYQYYVSFEPIVDCSFDGMT-LYLPKRLEGVPIIISFKLVKIVHLYNIIFKMRALNMCVIGRNYYMPSP-KEKLEVWPGYVTSMLLADVSHRVL-RTQTASHQQTCQDDISKLLVGNVVLTRYNNKCYRTFSLY--NGTFAEYYKRMYCLVPELSYITGLTDTMR--ADNRVMRELASHTRLNPTQRRDALRTYGRLLPIETIRFRWSRAVTKFMSMYRQCSRDMGIRVDRTESYMREIQLVVTIFPFQREDRYSAIKQLCCHDMPIPSQVINSRSVTQKIALQINCKLGGELWALNIPMVCGVDVTHDPAHHKS--SVVGFVSNINQTCTRWYSKVRMQQPGEEVNGLKICFTDALRKYYPSRVVIYRDGVGDGQLSVVRDYEVKQLIECCKPKLGVIVVQKRINTRLLNPRPGTVVDHTVTRNNWYDFFVVSQHIRQGTVSPTHYVVVYDGLDWKPDIVQALTYKMTHLYYNWPGTVRVPAPCQYAHKLAFLIGQNV
	Capitella_teleta_Piwi2               -----------MCF------------RS------------------------------------QPMLVHVPKKKDIQRQP---------------------------RGKPL----------------------------R-------------------LLVPEFCFLTGLSDSMK--ADFRVTKDLATHTRITPAQRHLSIRKFGRGIRPLDIVFGWGRQLGRFVQVMMQEAPKMGIRVVRNESYISQLQLVVCLMPRGRDDLYSAVKTLCCHEQPVPSQMIVSRSVVQKVILQINAKLGGELWSVAMPMVCGIDVYHDKLSGK---SIAALVSSMNPQCTRWYSRTCEQMSNQEVDSLGPCFVAAIKKFAPNRIVLFRDGVGDGQLAHVSQHEAAQLEKCYKPKLSIVVVQKRINQRIFNPPPGTVVDHTITRFNWYDFFLVSQHVRQGTVSPTHYVVVHDDSGWDCDRMQRLSYMLTHLYYNWPGTVRVPAPCQYAHKLAYLQGQNL
	Nematostella_vectensis_PiwiB         ------IPLSCNYIFQYCVSYSPEIDNRFDSTM-LFLPRRLPGSPVTVTITFTRVVQVYNILFRMKILDMQQVGRYFYNPKP-QHNLVLWPGYITSMLLCDVHHRLL-RTDSCKVGDRFRDEVSKELIGSVVLTTYNNKTYRTFTFH--NGTFVEYYKKSYLLIPELCKLTGLNDAMR--KDFRVMKDIAAHTRVNPMQRQEAMKKFGRQFPPEKIIYGWGRDATRFVQTMNKCAPAMGIQVDRTETYLRSIQVVVVIFPTSRDDRYAAVKKLCCVESPVPSQVIISKSVVQKIALQINVKLGGELWALDIPMVCGIDVYHDKARGGR--SVGGLVCSMNRSLTRWYSDVCFQSPGQEIDGLKMLLIKGIRKWHPERIIVYRDGVGDGQLKVVAGYEVQQFEECYKPKLAVLVVQKRINTRIFNPGPGSVLDHTVTRKDWNDFFLVSQHVRQGTVTPTHYVVVYDTSNWKPDIMQKLSYKLTHLYYNWPGTVRVPAPCQYAHKLAYQVGQSI
	Lottia_gigantea_Piwi2                --------MSANYIYQYHVSYNPQIDSKFDGAL-LFLPKKLPGADITLTIKLIKVVQVYNILLRMSILELCQVGRYYYNPHP-QHKLEVWPGYITAMLLLDASHRVL-RTETVNNPKGFKDEITKLVLGNIVLTRYNNKTYKTFPAS-KTGSFVDYYKTNYCLVPELCYMTGLSDAMR--SDFRLMKDIASHTRVTPAQRQMAMKKFGRLLPVENIHFDWGRESSNFIEMMSKVCPKMGIMIDRTETFIRTLQMVVIICPTSRDDRYSAIKKLCCCEQPVASQVINAKSVTTKIALQINCKLGGELWAVEIPMIIGIDVYHDVAKGKR--SIAGFVASMNKACTRYYSRCYFQGQNQEIDGLKICLISALRKYHPDKIVIYRDGVGDGQLRAVADYEVKQLHTCYNPNMALVIVQKRINTRLFNPPPGSVMDHSITRRDWYDFFLVSQHVRQGTVSPTHYIVVHDGTGLTVDRMQRLTYKMTHLYYNWPGTVRVPAPCQYAHKLAYLVGQSI
	Capitella_teleta_Piwi1               GDYGNRVCMYTNTFCQYHVNYTPPVESRFDGMI-LYLGRKLDGDNIKVSIEFTGEVQILNIMMRLGALGMKLIGKNYFMPSP-QYGYEIYPGYTTAQLICDVAHKIL-RPHSVARGGNFHENCTKKLVGEIVMTTYNNKTYRTFKQR--DGTFNEYYKKQYLLVPELCRLTGISEEAR--ADFMVMKNLSVFTRVSPQGRMERLIEFGRNLGCERLVQGWSREMRGLHETLRRVSGAMGMRLGYTQTFINALQLVVCVLPSNKKDLYDSIKKFCCCEKPVPSQCIIQRSVCTKVAVQMNCKLGGEVWAVDIPMVIGIDTYHDSAQKGR--SIGAFVASTNNTLTKYYSRCCFQTTGQEMSGLQICMRDALKHYMPERVFIYRDGVGDGQVDHVANSEVKQIMECYAPKTAVIIVTKRINNRFFNPLPGTVVDTVVTRPEWYDFFLVSQSVRQGTVSPTHYNVVHDSSGMSPNHLQRLTYKLCHLYYNWPGTIRVPAPCQYAHKLAFLVGQSL
	Mus_musculus_Piwi1                   GSSGIIVKLSTNHFYQYHIDYNPLMEARFDGTI-LFLPKRLQGEHVRITITLTNELQFYNIIFRLKIMNLQQIGRNYYNPSP-NHRLVIWPGFTTSMLCTDVSHKVL-RSETVTEEHKFQEQVSKELIGLIVLTKYNNKTYRTFKKA--DGSFLEYYRKQYMLIPELCYLTGLTDKMR--NDFNVMKDLAVHTRLTPEQRQREVGRLGRILQSEKIHQGWSKETRGLIQNLFKVTPAMGIQMDRTEAYLRALQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARAIATKIALQMNCKMGGELWRVDMPMIVGIDCYHDTTAGRR--SIAGFVASINEGMTRWFSRCVFQDRGQEVDGLKVCLQAALRAWSPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCYNPRLTVIVVKKRVNARFFNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSI
	Nematostella_vectensis_PiwiA         GSVGTKVGIVSNYFYQYNVSFSPEVDFKFDGMV-LFLPKKLPDEPVEITITLTNELHLFNIILKLKYIDMKQVGRNYYYIKP-KHGLELWPGYTTSLLGADVSHKVL-RKQTVQGRGNFHDEAAKKLVGEIVLTRYNNKTYRTFTTP--KGSFNDYYKKNYLLLPELCFLTGLSDDMR--ADFSVMKDLAVHTRLDPKARSKDLNKLGRILPGEKIFQKWSREIRGFQQTLARVCGPMGINTDRTDSYLKALQIVVTIVPSNRKDRYDSIKKLCCLEKGVPSQVVVSRSVCTKIGIQLNCKLGGEAWAVDIPMIVGIDTYHDSSQKGR--SVGGFVASLNKPCTRYYSRCTFQHSGQEVDGLKVCMTAALRQWQPDKIIVFRDGVGDGQLRAVIEHEVPQILSTYDPKFGFTVVKKRIQTRLFNPPPGTVVDKEVTRSEWFDFFIVSQSVRQGTVSPTHYNVIHDTTGLRPDHWQQLSYKLTHLYYNWPGTVRVPAPCQYAHKLAFLVGQSL
	Lottia_gigantea_Piwi1                GSAGKPIALLANFFYQYQVDFNPSIDSRFDGGI-LYLPKKLQDTKIQITITLTNELQVVNIIFRLAAIEMKEIGRHYFNPSP-RHKLSVMPGFATSLLGADIAHKII-RTDSVRGGGSFYDKVFKALVGSIVLTRYNNKTYRKFKLH--NGSYVDYYKKNYYLLPELCTLTGLSDDVR--ADFSVMKDVATHTRIAPEGRCKKLTDFGRVLPPEAISQGWSRDMRGLVDTLIRVGGPMGMRIDRNETFLETLQMVLIVVPNNKKDRYDAIKKFCCVEHPVPSQVVVARSVATKICIQMNCKLGGEVWTVEIPMVIGIDTYHDSSQKGK--SVGGVIASLNTNLTRFYSTVTHQTQHQEIDGLVASLNGALRKYHPEKIIVYRDGVGDGQLPTVFGHEVPQLLEAYNPKLAVIVVKKRINARFFNPVPGTCVDNEVTKPEWYDFFLVSQSVRQGTVTPTHYNVIWDQTGLKPDHMQRLSYKLTHLYYNWPGTIRVPAPCQYAHKLAFLVGQSL
	Bombyx_mori_Arg2                     SPPSRTVPILTNYLYRYDVTFKPDK-PKFDQTKNCYSLTPLPDMPFEVSFKASGIVQCIDIVLKGTLESYVKAGRQYFMRPDLGDGLEMWTGLFQSFINVDVAHKGFPKNQPMPGRAAE--AFNEFIRGKILGTGPSSGQLRTFTLENDKGTVYEYFMKEKYLPMELVEVAYGQARNKQLNDRQLSTMVREAA-TPPDVRKRKIEEVAKILEAPTLEVGW----QAIVSKLMNTGRQMGMNVDLHKSMLHALNFLVVVVSGRGRDYYHKLKQIAELKVGILTHVFKEDQTARNILLKVNSKLMGINQALENRMIVGADVTHPSPDQSNIPSIAAVTASMDTKCYIYNIELSIQTPKKEIVQFEDIMVDHFHAFKPKKVFVFRDGVSEGQFAEVMKSELTGLHRAAKPEVLFILVQKRHHTRFFNVDPGTVVDRDIVHPRELDFYLVSHQAIKGTARPTRYHAVCNDGRIPENEVEHLAYYLCHLYARCMRAVSYPAPTYYAHLACLRARSLT
	Tribolium_castaneum_Arg2a            GVKGRPIKIESNHLYHYDVSITPD-TPKFDGRKNLYSPKKLPKRGFKVVVKLARTVQCLDIVLRAPSNSCISSGRCFFTPPRLGDGMEMYYGFYQSLLNVDVVHKAFPEALNVPLTDFVQKALEKFLKQEIPGQSGSRRIFR---FTLGDGTVEKYYQEVKLIPLEFCTVVSGQVVNRKMNENQTSVMIKKAATSTDV-RKDKIMQVGRVLQPPTLLYAWRADM--LADMIFRMASSNGMQIQNLRDIIDYFDLIIVVVP-NSGPQYSLVKQAAELNVGCLTQCIKERQIIANILLKINSKLNGTN-HILSSIIMGADVTHPGPDAKDVPSVAAVTASHDPNAFQYNICWRLQPPKVEIEDLCAITVEQLMFFYPETIVFFRDGVSEGQFAEVRRAEISAIHQAYEPRITFLVVQKRHHTRLFNVPAGTCVDTHITNPMMQDFYLVSHASIQGVAKPTKYCTLWDDNNMSNDDIEELTYYLCHMFTRCNRSVSYPAPTYYAHLAAARAKVYV
	Tribolium_castaneum_Arg2b            GTKGRRIQIESNHLYHYDVAITPD-TPKFDGRKNLYSPKKLPKKEFKVEVKLARTVQCLDIVLRAPSNACIIAGRCFFTPPPLGDGMELYYGFYQSLLNVDVAHKAFPKASNVPLREFVQRDFEKFIKQEIPNQSSSKRIHR---FKLDDGTVERYYQEVKLLPLEFCTVVGGQAINRKMNENQTSAMIRKAATSTDV-RKDKIMQTARVLNPPSLLYAW--RADRLADMIFRMASSNGMQIQGLRDFIDYFDLIIVVVP-NSGPQYSFVKQAAELNVGCLTQCIKERQTVGNILLKINSKMNGTN-HRLSPMIMGADVTHPSPDARDIPSVAAVTASHDPNAFQYNICWRLQPPKVEIEDLCNITVEQLKFFYPESIVFFRDGVSEGQFKQVQRAEIAAIQKAYEPKITFLVVQKRHHTRLFNVPAGTCVDTHITNPRMQDFYLVSHASIQGVAKPTKYCTLWDDNNMNNDDIEELTYHLCHMFTRCNRSVSYPAPTYYAHLAAARAKVYI
	Nematostella_vectensis_Arg1          GSTGRTIALRANFFHHYDLSICPDKCPRFDGRKNLYTRDPLPDKKFKVTLKWVSQVQALDVVLRLPSMKYTPVGRSFFSPPPLERGREVWFGFHQSLLNIDVSATAFYKCQSVPLNDADRLRFTREIKGEITHCGPMKRKYRQFPLTLESGTVARYFQDKHYLPLEVCNIVPGQRCVKKLTDTQTSKMIRATARSAPD-REREIRGLGRVLPPPKLVYGW--DMRGFSNQLMKISVEQGMPIDEVERMFRRLQMILVVLP-GKTPVYAEVKRVGDTMLGVITQCIQGKQTLSNLCLKINAKLGGVN-NILAPIFLGADVTHPAAGDDKRPSVAAVVGSMDAHLSRYYASVRVQTHRQEIAELAAMVRELLVQFYPQRIVFYRDGVSEGQFRQVLVHELKAIREAYQPGISFIVVQKRHHTRLFNVPPGTTVDRGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNFSADELQALTYQLCHTYVRCTRAVSIPAPAYYAHLVAFRARYHM
	Helobdella_robusta_Arg2              GSEGRKILLKANHFYHYDVAVMPDKCPRYDGRRNMYSKEPLMERVFKVTI------------------RYSAANAQVMFNPTLGG----------------VSATAFYKAQPVPLSDAQRVKFTKEIRG-------------DFPLQLERGTVAKYFLEKYYLPLEVCNIVHGQKCIKKLTDSQTSTMIKSTSRAAPD-REREINTLGRVLPVPKLQM--------FTQQLQKISTDAGMPVDQI-------TFLF-----AKSSSPSEVKRVGDILFGLATQCVQSKQTLSNLCLKIN-------------IFLGADVTHPPAGDRSKPSIAAVVGSMDAHPSRYSATVRIQQHRQEIQDLSVM---------PCRY----------------------------PGGSFV-----------------------------------HSSIH------------------------------------------------------------
	Strongylocentrotus_purpuratus_Arg1   GIDGRPIALRANHFFHYEVNITPEKCPRFDGRTNMYCKDEIPDRIFKASIKFEGKVQALDVIMRLPSLRYTPVGRSFFSPPPLGGGREVWFGFHQSMLNIDVSATAFYSCQSVPLSDSHRVKFTKEIKGEITHCGNMKRKYRTFPWQLENGTVAKYFRERHYLPLEVCNIVAGQRCIKKLTDLQTSTMIKATARSAPD-REKEIKSLGRVLPAPKIQYGW--DMRGFTLQLQKISNEAGMPIDQVEAMFRHLQLIIVVLP-GKTPVYAEVKRVGDILLGIATQCVQVKQTLSNLCLKIKVKLGGVN-NILAPIFCGADVTHPPAGDDKKPSIAAVVGSMDGHPSRYCASVRIQTHRVEIQDLQAMVKELLVEFYPARIIMYRDGVSEGQFLQVLANEMNAIRDAYEPGITFIVVQKRHHTRLFNIPAGTTVDSGITHPLEYDFYLCSHAGIQGTSRPSHYHVLWDDNGFKADELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
	Bombyx_mori_Arg1                     GHEGRPIMLRANHFHHYDVNIQPDKCPRFDGRNNLYTRDPLPDRVFRVTIKWVAQVLALDVVMRLPSMMYTPVGRSFFSSPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGTMKRKYRSFPLQLENGTVAKYFLDKYYLPLEVCNIVPGQRCIKKLTDMQTSTMIKATARSAPD-REREINNLGRVLPPPKLQYGW--DMRGFTQQLQKISNDAGMPIDQVEPMFKYLQLVVVVLP-GKTPVYAEVKRVGDTVLGMATQCVQAKQTLSNLCLKINVKLGGIN-SILVPIFLGVDVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEVHEMSSMVQELLIMFYPHRIIMYRDGISEGQFLHVLQHELTAVREAYKPGITFIVVQKRHHTRLFNIPAGTTVDLGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFGSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
	Tribolium_castaneum_Arg1             GREGRPIGLKANHFHHYDVSIQPDKCPRFDGRNNLYTRDPLPDRLFRVTIKWVAQVLALDVVMRLPSMSYTPVGRSFFSSPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGTMRRKYRSFPLQLDNGTVAKYFLDKYYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPD-REREINNLGRVLPPPKLQYGW--DMRGFTQQLQKISNDAGMPIDQVEPMFRYLQLVVVVLP-GKTPVYAEVKRVGDTVLGMATQCVQAKQTLSNLCLKINVKLGGIN-SILVPIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIQELSSMVRELLIMFYPHRIILYRDGVSEGQFLQLLQHELTAIREAYKPGITFIVVQKRHHTRLFNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDSHLDSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
	Lottia_gigantea_Arg                  GTEGRPILLRANHFHHYDVSITPDKCPRFDGRKNLYSREPLPDRVFTVAIKWVAQVQALDVVMRLPSMTYTPVGRSFFSQPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGTMKRKYRSFPLQLDSGTVARYFHEKYYLPLEVCNIVGGQRCIKKLTDMQTSTMIKATARSAPD-REKEINNLGRVLTPPKIQYGW--DMRGFTSQLQRISNDAGMPIDQVEPMFRYLQLIVVVLP-GKTPVYAEVKRVGDILFGLATQCVQSKQTLSNLCLKINVKLGGIN-SILLPIFLGADVTHPPAGDCMKPSIAAVVGSMDAHPSRYSATVRVQQHRQEIPELCNMIRELLIQFYPTRIIFYRDGVSEGQFQTVLSSELRAVREAYQPGITFIVVQKRHHTRLFNIPAGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
	Capitella_teleta_Arg1                GVEGRHIMLRANHFHHYEISIQPDKCPRFDGRKNLYCRDPLPDRVFKVSIRWMAQVQALDVVMRLPSMTYTPVGRSFFSPPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGTMKRKYRSFPLQLESGTVAKYFLERYYLPLEVCNIVGGQRCIKKLTDMQTSTMIKATARSAPD-REKEINSLGRVLPAPKLQYGW--DMRGFTQQLQRISNDAGMPIDQVEPMFRYLQLIVVVLP-GKTPVYAEVKRVGDIMFGLATQCVQSKQTLSNLCLKINVKLGGIN-NILLPIFLGADVTHPPVGDRSKPSIAAVVGSMDAHPSRYSATVRVQSHRQEISELSSMVKELLIQFYPYRIIFYRDGVSEGQFTQVLHHELRAVREAYHPGITFIVVQKRHHTRLFNIPAGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFSADELQSLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
	Helobdella_robusta_Arg1              GNEGQPICLRANHFNHYDVTISPDKCPRFDGRKNLYSRDQLPDRVFHVSIKFVAQVQALDVVMRLSSMTFQ--GRSFFSPPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGAMKRKYRSFPLQLESGTVAKYFQERYYLPLEVCNIVHGQRCIKKLTDLQTSTMIKATARSAPD-REKEINNLGRILPVPKLQYGW--DMRGFTQQLQKISTDAGMPIDQVEPMFRYLQLIVVVLP-GKTPVYAEVKRVGDILFGLATQCVQSKQTISNLCLKINVKLGGIN-SILLPIFLGADVTHPPAGDRSKPSIAAVVGSMDAHPSRYSATVRIQQHRQEIQELSAMVKELLIQFYPTRIIFYRDGVSEGQFAHVLSYELRAIREAYQPGISFIVVQKRHHTRLFNIPAGTTVDIGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTADELQMLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
	Mus_musculus_Arg4                    GTVGKPIRLLANHFYHYDVDIKPEKRPRYDGKRNMYTAHPLPDQTFKVSVQWVSVVQALDVITRLPSMRYTPVGRSFFSPPPLGGGREVWFGFHQSMLNIDVSATAFYRAQPIPLTDSQRVKFTKEIRVESTHG-----KYSKYSLRILTE------HGKYYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPD-RQEEISRLGRVLPAPMLQYGW--DMRGFTDQLRKISKDAGMPIDSVEPMFKHLQLIVVILP-GKTPVYAEVKRVGDTLLGMATQCVQVKQTLSNLCLKMNAKLGGIN-NVLVPIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEVQDLTSMARELLIQFYPTRIIYYRGGVSEGQMKQVAWPELIAIRKAYRPGITYIVVQKRHHTRLFNVPAGTTVDSTVTHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHL
	Mus_musculus_Arg2                    GTTGRTIKLQANFFYHYELDIKPEKCPRFDGRKNLYTAMPLPDRIFKVSIKWVSCVQALDVVMRLPSMRYTPVGRSFFTASPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVKFTKEIKGEITHCGQMKRKYRTFPLQQESGTVAQYFKDRH-----VCNIVAGQRCIKKLTDNQTSTMIRATARSAPD-RQEEISKLGRVLQPPSILYGW--DMRNFTEQLRKISRDAGMPIDSVEPMFRHLQLVVVILP-GKTPVYAEVKRVGDTVLGMATQCVQMKQTLSNLCLKINVKLGGVN-NILLPIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIQDLAAMVRELLIQFYPTRIIFYRDGVSEGQFQQVLHHELLAIREAYQPGITFIVVQKRHHTRLFNIPAGTTVDTKITHPTEFDFYLCSHAGIQ---------------------------------VRR------------------------
	Mus_musculus_Arg1                    GTVGKPIKLLANYFYHYEVDIKPDKCPRYDGKKNIYTVTALPDRIFKVSIKWLAIVQALDVAMRLASMRYTPVGRSFFSPPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSQRVRFTKEIKGEVTHCGQMKRKYRTFPLQLESGTVAQYFKQKYYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPD-RQEEISRLGRVLPAPILQYGW--DMRGFTDQLRKISKDAGMPIDSVEPMFRHLQLIIVILP-GKTPVYAEVKRVGDTLLGMATQCVQVKQTLSNLCLKINVKLGGIN-NILVPIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIEDLSYMVRELLIQFYPTRIIFYRDGVPEGQLPQILHYELLAIRDAYQPGITYIVVQKRHHTRLFNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHL
	Mus_musculus_Arg3                    GTMGKPIKLLANCFYLYEVDIKPDKCPRYDGKRSLYTANPLPDRPFKVSVKFVSRVHAVDVVLRLPSMKYTPVGRSFFSAPPLGGGREVWFGFHQSMLNIDVSATAFYKAQPVPLTDSHRVKFTKEIKGEVTHCGTMRRKYRTFPLQLENGTVAQYFREKYYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPD-RQEEISRLGRVLPAPMLQYGW--DMRGFTDQLRKISKDAGMPIDSVEPMFRHLQLIIVILP-GKTPVYAEVKRVGDTLLGMATQCVQVKQTLSNLCLKINVKLGGIN-NILVPIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIQDLASMVRELLIQFYPTRIIFYRDGVSEGQFRQVLYYELLAIREAYQPGITYIVVQKRHHTRLFNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNFFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL
;
END;

BEGIN TREES;
	Title 'Piwi-Argonaute';
	LINK Taxa = Taxa;
	TRANSLATE
		1 Tribolium_castaneum_Piwi,
		2 Bombyx_mori_Piwi,
		3 Helobdella_robusta_Piwi1,
		4 Mus_musculus_Piwi2,
		5 Strongylocentrotus_purpuratus_seawi,
		6 Helobdella_robusta_Piwi2,
		7 Capitella_teleta_Piwi2,
		8 Nematostella_vectensis_PiwiB,
		9 Lottia_gigantea_Piwi2,
		10 Capitella_teleta_Piwi1,
		11 Mus_musculus_Piwi1,
		12 Nematostella_vectensis_PiwiA,
		13 Lottia_gigantea_Piwi1,
		14 Bombyx_mori_Arg2,
		15 Tribolium_castaneum_Arg2a,
		16 Tribolium_castaneum_Arg2b,
		17 Nematostella_vectensis_Arg1,
		18 Helobdella_robusta_Arg2,
		19 Strongylocentrotus_purpuratus_Arg1,
		20 Bombyx_mori_Arg1,
		21 Tribolium_castaneum_Arg1,
		22 Lottia_gigantea_Arg,
		23 Capitella_teleta_Arg1,
		24 Helobdella_robusta_Arg1,
		25 Mus_musculus_Arg4,
		26 Mus_musculus_Arg2,
		27 Mus_musculus_Arg1,
		28 Mus_musculus_Arg3;
	TREE Tree1 = (((14:0.69092196,(15:0.09445217,16:0.032704525):0.2883823):0.36795288,(17:0.095053464,(19:0.099542074,((26:0.05898812,(28:0.03404981,(27:0.03576933,25:0.08949306):0.004626909):0.033975575):0.041197438,(((24:0.029087132,18:0.15686427):0.02776071,(22:0.033541795,23:0.04000804):0.0055317027):0.017289687,(21:0.012476142,20:0.020393932):0.051968217):0.012954204):0.031719916):0.06884004):0.22139719):0.55056375,((1:0.31799605,2:0.33890563):0.184902,(((3:0.52701986,7:0.34767953):0.09683569,((12:0.25649935,5:0.34929433):0.04159807,13:0.25650424):0.03889807):0.024014747,(11:0.3272512,(((9:0.20676933,6:0.38803723):0.042747773,(10:0.38270828,8:0.19949777):0.068521775):0.07420973,4:0.4703359):0.10803991):0.048505317):0.12712966):0.47715527);
END;