@ARTICLE{TreeBASE2Ref15598,
author = {Alexandra M. Gottlieb and Gustavo C Giberti and Lidia Poggio},
title = {Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and ITS sequence data.},
year = {2003},
keywords = {},
doi = {},
url = {},
pmid = {},
journal = {American Journal of Botany},
volume = {},
number = {},
pages = {},
abstract = {Genetic relationships among 120 individuals, representing the seven native South American species of Ilex, were surveyed by amplified fragment length polymorphism (AFLP). Additionally, a phylogenetic analysis using sequences of internal transcribed spacer regions (ITS) of 35 further species, was accomplished through direct character optimization. Moreover, a sensitivity analysis was performed involving 15 indel-substitution models. Information derived from these analyses contributed to clarify the relationships among South American representatives of the genus. The Neighbor-joining clustering of AFLP markers rendered groupings that were in accordance with morphological identification of the accessions. Phylogenetic analysis revealed that ITS sequences of South American taxa were not monophyletic. Likewise, ITS sequences of both North American and Asian taxa were not monophyletic. In addition, the sensitivity analysis showed that the placement of several taxa was highly sensitive to parameter variation, whereas other taxa formed stable clades, which were associated according to their geographical origin. The AFLP phenogram and the consensus cladogram were remarkably congruent in that both demonstrate the existence of a group formed by I. brasiliensis, I. theezans, I. integerrima, I. brevicuspis and I. argentina, and that I. dumosa and I. paraguariensis were unrelated to the former group.}
}
Citation title: "Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and ITS sequence data.".
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