TreeBASE Search-Matrices for study 19045

@ARTICLE{TreeBASE2Ref25688, author = {Eric C. Otto and Robert A Blanchette and Benjamin W Held and Charles Wesley Barnes and Mary Obodai}, title = {Ganoderma mbrekobenum}, year = {2016}, keywords = {Ganoderma, Africa, Ghana}, doi = {}, url = {http://}, pmid = {}, journal = {Persoonia}, volume = {36}, number = {}, pages = {416--417}, abstract = {Mature Basidiomata annual, pileate, stipitate, dimidiate, applanate, woody to corky when dried, homogenous context structure, pileus maroon to liver brown when dry, surface hard and glabrous, margin rounded, thickened, maroon to liver brown when dry. Stipe substibe (>5 cm), lateral, columnar, with one solitary column, maroon; borders with hymenophore thickened. Pore surface smooth, creamy to snuff brown when dry, pores 4-6 per mm, round to somewhat irregular and slightly elongated, 105-247 x 76-207 ?m (av. 167.2 x 123.8 ?m; SD 32, 26; n = 100), dissepiments 44-152 ?m (av. 83.6 ?m; SD 23; n = 100); tubes 0.1-0.7 mm long, dark brown. Hyphal system dimitic; generative hyphae slightly inconspicuous, branched, thin-walled, and hyaline; skeletal hyphae most prevalent in the basidiocarp, occasionally branched, pale to dark brown, 2.5-7 ?m thick, tapering towards the end. Basidia not observed. Basidiospores brown, ovoid to broadly ellipsoid with a truncate base, bitunicate, verruculose, 8-11.5 x 6-8 ?m (av. 10.4 x 7.1 ?m; SD 0.7, 0.4; n = 100), perisporium thin, smooth; exosporium with intermediate thick inter-walled pillars; endosporium thick, dark brown. Chlamydospores not observed. Culture characteristics ? No live culture obtained. Typus. GHANA, Brong Ahafo and Greater Accra Regions, on angiosperms, June 2015, M. Obodai, holotype MIN 850481, paratype MIN 850482, holotype ITS sequence GenBank KX000896 and LSU sequence GenBank KX000897, paratype ITS sequence GenBank KX000898 and LSU sequence GenBank KX000899, holotype MycoBank MB 816172. Notes ? Ganoderma mbrekobenum causes decay in the roots and trunks of angiosperm trees in the southern regions of Ghana. Sequences were downloaded from GenBank for phylogenetic analysis with G. mbrekobenum sequences using the program Geneious R9 (http://www.geneious.com, Kearse et al., 2012). The complete ITS sequence of the G. mbrekobenum holotype was used for the Blastn search. The results gave the highest score to an isolate Ganoderma sp. (EGDA, LN774971) from Egypt, with a single nucleotide difference. The next 14 Blastn hits were to Ganoderma sp sequences from a single institution in India. The analysis included only the top three of these sequences, having four to six differences from the G mbrekobenum holotype. A few isolated sequences with various Ganoderma species names had relatively high Blastn scores, but were excluded from the analysis because they did not align with their respective species and are likely G. mbrekobenum, or closely related. The closest legitimate Ganoderma species were G. applanatum and G. fornicatum, both with 94% identity. Additional sequences of other recently described Ganoderma species from Africa were included in the analysis. The final alignment was edited by hand for alignment errors. } }