TreeBASE Search-Matrices for study 26426

@ARTICLE{TreeBASE2Ref30910, author = {Joyce H.C. Woudenberg and Bram Hanse and Gerard van Leeuwen and Johannes (Ewald) Zacharias Groenewald and Pedro W. Crous}, title = {Stemphylium revisited}, year = {2017}, keywords = {Morphology, Multi-gene phylogeny, Pleospora.}, doi = {10.1016/j.simyco.2017.06.001}, url = {http://dx.doi.org/10.1016/j.simyco.2017.06.001}, pmid = {28663603}, journal = {Studies in Mycology}, volume = {87}, number = {}, pages = {77?103}, abstract = {In 2007 a new Stemphylium leaf spot disease of Beta vulgaris (sugar beet) spread through the Netherlands. Attempts to identify this destructive Stemphylium sp. in sugar beet led to a phylogenetic revision of the genus. The name Stemphylium has been recommended for use over that of its sexual morph, Pleospora, which is polyphyletic. Stemphylium forms a well-defined monophyletic genus in the Pleosporaceae, Pleosporales (Dothideomycetes), but lacks an up-to-date phylogeny. To address this issue, the internal transcribed spacer 1 and 2 and intervening 5.8S nr DNA (ITS) of all available Stemphylium and Pleospora isolates from the CBS culture collection of the Westerdijk Institute (N = 418), and from 23 freshly collected isolates obtained from sugar beet and related hosts, were sequenced to construct an overview phylogeny (N = 350). Based on their phylogenetic informativeness, parts of the protein-coding genes calmodulin and glyceraldehyde-3-phosphate dehydrogenase were also sequenced for a subset of isolates (N = 149). This resulted in a multi-gene phylogeny of the genus Stemphylium containing 28 species-clades, of which five were found to represent new species. The majority of the sugar beet isolates, including isolates from the Netherlands, Germany and the UK, clustered together in a species clade for which the name S. beticola was recently proposed. Morphological studies were performed to describe the new species. Twenty-two names were reduced to synonymy, and two new combinations proposed. Three epitypes, one lectotype and two neotypes were also designated in order to create a uniform taxonomy for Stemphylium. } }