TreeBASE Search-Matrix of study 1087

@ARTICLE{TreeBASE2Ref17934, author = {Jan M van Niekerk and Pedro W. Crous and Johannes (Ewald) Zacharias Groenewald and Paul H. Fourie and Francois Halleen}, title = {DNA phylogeny and morphological characterization of Botryosphaeria species occurring on grapevines.}, year = {2003}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {Several species of Botryosphaeria are known to occur on grapevines, causing a wide range of disorders including bud mortality, dieback, brown wood streaking and bunch rot. In the present study the 11 Botryosphaeria spp. associated with grapevines growing in various parts of the world, but specifically in South Africa, were distinguished based on morphology, DNA sequences (ITS-1, 5.8S, ITS-2 and EF-1a) and pathological data. Botryosphaeria australe, B. lutea, B. obtusa, B. parva, B. rhodina and a Diplodia sp. were confirmed from vines in South Africa, while Diplodia porosum, Fusicoccum viticlavatum and F. vitifusiforme were described as new. Although isolates of B. dothidea and B. stevensii were confirmed from grapevines in Portugal, none of these two was found among the isolates obtained in South Africa, nor were any isolates of B. ribis confirmed from grapevines. All grapevine isolates formerly presumed to be B. ribis, were identified as B. parva based on their EF-1a sequence data. From artificial inoculations on grapevine shoots, we conclude that B. ribis, B. australe, B. stevensii and B. parva were more virulent that the other species studied. The Diplodia sp. collected from grapevine canes was morphologically similar, but phylogenetically distinct from D. sarmentorum. Diplodia sarmentorum was confirmed as anamorph of Otthia spiraeae, the type species of the genus Otthia (Botryosphaeriaceae), which phylogenetically clustered within Botryosphaeria and is therefore synonymized with it. These findings confirm earlier suggestions that the generic concept of Botryosphaeria should be expanded to include genera with septate ascospores and Diplodia anamorphs.} }