@ARTICLE{TreeBASE2Ref17920, author = {Gert J van Coller and Sandra Denman and Johannes (Ewald) Zacharias Groenewald and Sandra C. Lamprecht and Pedro W. Crous}, title = {Characterisation and pathogenicity of Cylindrocladiella spp. associated with root and cutting rot symptoms of grapevines in nurseries}, year = {2005}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Australasian Plant Pathology}, volume = {}, number = {}, pages = {}, abstract = {Species of Cylindrocladiella occur on a variety of hosts, where they are known to act as pathogens or saprobes. Eight species from this genus are currently recognised, of which five have been reported previously in South Africa, namely C. camelliae, C. elegans, C. lageniformis, C. parva, and C. peruviana. In a recent survey of soilborne diseases of nursery grapevines in the Western Cape province of South Africa, various isolates of Cylindrocladiella were obtained from a newly established 99-Richter mother block exhibiting decline symptoms. The DNA phylogeny of these and additional isolates, also obtained from declining grapevines, was determined by sequencing the ITS (ITS1, ITS2 and 5.8S) as well as ?-tubulin and histone H3 gene regions. Results from this study revealed that four species of Cylindrocladiella occur on grapevines in South Africa, namely C. lageniformis, C. parva, C. peruviana, as well as a new species described here as C. viticola, which forms part of the C. infestans species complex. Pathogenicity trials, using stem inoculations on green and one-year-old 99-Richter cuttings were conducted to determine the pathogenicity of selected isolates of these species, but these results were inconclusive.} }

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