@ARTICLE{TreeBASE2Ref23760,
author = {Laishram Shantikumar Singh and Hemant Sharma and Narayan Chandra Talukdar},
title = {Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak lake of Manipur, India},
year = {2014},
keywords = {Phoomdi, Streptomyces sannanensis SU118, Antibacterial potential, Minimum inhibitory concentration},
doi = {},
url = {http://},
pmid = {},
journal = {BMC Microbiology},
volume = {},
number = {},
pages = {},
abstract = {Background: Actinomycetes have provided a wealth of bioactive secondary metabolites with interesting activities such as antimicrobial, antiviral and anticancer. The study aims at isolation, characterization and the antimicrobial potentiality of Streptomyces sannanensis SU118 obtained from Phoomdi, a unique habitat of Loktak Lake of Manipur, India.
Results: An actinomycete strain isolated from Phoomdi soil of Loktak Lake of Manipur, India was identified as Streptomyces sannanensis SU118. It is a Gram-positive filamentous bacterium which exhibits antimicrobial activity only against Gram-positive bacteria, while Gram-negative organisms were not affected. Glucose Soyabean meal broth was found to be the suitable medium for antibiotic production at 280 C for seven days of incubation. The antimicrobial agent produced by the strain was extracted with ethyl acetate as solvent and purified by thin layer chromatography. Screening and bioassay - guided fractionation of the ethyl acetate extract from the culture filtrate led to the isolation of an active potential compound (Rf value 0.56) with λmax 275.0 nm which has got the lowest minimum inhibitory concentration (0.5 ?g/ml) against Staphylococcus aureus MTCC 96 and Staphylococcus aureus (clinical isolate), whereas highest (3.0 ?g/ml) was recorded against Mycobacterium smegmatis MTCC 6 and Bacillus circulans MTCC 8074.
Conclusion: This study has therefore uncovered the potential of exploring virgin untapped habitats in the Indo-Burma biodiversity hot spot region as reservoir of promising antimicrobial metabolite producer. These results highlighted the scope for further characterization of the metabolite and could be a candidate in the generation of new antimicrobial agents.
}
}
Matrix 24498 of Study 16506
Citation title:
"Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak lake of Manipur, India".
Study name:
"Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak lake of Manipur, India".
This study is part of submission 16506
(Status: Published).
Matrices
Title: Streptomyces sannanensis strain SU118
Description: MSA of the sequences
Rows
|
Taxon Label |
Row Segments |
Characters 1?–30 |
| Streptomyces sannanensis SU118 |
(none)
|
------------------------------ |
| Streptomyces phaeochromogenes strain NBRC 13511 |
(none)
|
---------------------ACGAACGCT |
| Streptomyces violaceorectus strain NBRC 13102 |
(none)
|
---------------------ACGAACGCT |
| Streptomyces viridobrunneus strain LMG 20317 |
(none)
|
--------------------GACGAACGCT |
| Streptomyces gulbargensis strain DAS 131 |
(none)
|
----------------------CGAACGCT |
| Streptomyces venezuelae strain 3495 |
(none)
|
AGAGTTTGATCCTGGCTCAGGACGAACGCT |
| Streptomyces omiyaensis strain NRRL B 1587 |
(none)
|
-------------------GGACGAACGCT |
| Streptomyces fradiae strain NBRC 13007 |
(none)
|
---------------------------GCT |
| Streptomyces litmocidini strain NBRC 12792 |
(none)
|
---------------------------GCT |
| Streptomyces bikiniensis strain DSM 40581 |
(none)
|
AGAGTTTGATCCTGGCTCAGGACGAACGCT |
| Streptomyces lateritius |
(none)
|
--------------------GACGAACGCT |
| Streptomyces nashvillensis Strain 1534 |
(none)
|
------------------------AGGGGG |
| Streptomyces sannanensis strain HBUM174756 |
(none)
|
------------------------------ |
Columns
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