@ARTICLE{TreeBASE2Ref25927, author = {Melvin R Duvall and Shrirang R. Yadav and Sean Vincent Burke and William P. Wysocki}, title = {Evolution of the micrairoid grasses (Micrairoideae; Poaceae): a plastome phylogenomic analysis}, year = {2016}, keywords = {}, doi = {}, url = {http://}, pmid = {}, journal = {American Journal of Botany}, volume = {}, number = {}, pages = {}, abstract = {Studies of complete plastomes are informative for our understanding of the systematics of grasses. Here we investigate the plastome phylogenomics of Micrairoideae. Micrairoideae comprise ten recognized genera, some of which are severely impacted by habitat fragmentation and are threatened with extinction. An independent origin of C 4 photosynthesis has been documented to have taken place within this subfamily. Complete plastomes were sequenced with next-generation sequencing-by-synthesis methods from Eriachne armitii, Hubbardia diandra, Limnopoa meeboldii, and the arundinoid outgroup Arundo donax. Plastomes were assembled by de novo methods, and the unambiguously aligned coding and noncoding sequences of the entire plastomes were analyzed phylogenetically with three recently published micrairoid plastomes and 14 outgroup species. Phylogenomic analyses showed strong support for ingroup micrairoid relationships, including the paraphyly of Hubbardieae with Isachneae. Anatomical, biochemical, and positively selected sites data are ambiguous with regard to the photosynthetic pathways in the subfamily. However, based on δ 13 C measurements, Hubbardia, Isachne, and Limnopoa were definitively shown to be C 3 taxa and Eriachne was indicated to be C 4 . The incongruence between a recent classification for Micrairoideae and our plastome phylogenomic results suggests the need for reclassification of tribes within the subfamily. The newly presented δ 13 C measurement for Eriachne suggests that further study is needed to indicate why anatomical and physiological characters exist in this and other micrairoid taxa in unexpected combinations.} }

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