TreeBASE Search-Matrix of study 21210

@ARTICLE{TreeBASE2Ref27355, author = {Vadim Y Kryukov and Oksana G Tomilova and Olga N Yaroslavtseva and Ting-Chi Wen and Natalia A Kryukova and Olga V Polenogova and Yuri Tokarev and Viktor V Glupov}, title = {Temperature preferences of Cordyceps militaris and its ability to kill insects under different thermo-physiological states}, year = {2017}, keywords = { Cordyceps; ecological preferences; virulence; temperature}, doi = {}, url = {http://}, pmid = {}, journal = {Mycoscience}, volume = {}, number = {}, pages = {}, abstract = {Genetic relationships and temperature preferences were determined for 19 isolates of Cordyceps militaris from the Palearctic temperate zone. Two intraspecific groups were revealed using the elongation factor tef1-alpha gene sequence. The variability of surface mycelial growth of isolates from different regions (Siberia, Far East, European part or Russia) and genetic lineages was extremely low at temperatures of 530 ?C. However, a correlation between latitude of origin and growth under warm conditions (27.5 ?C) was revealed. C. militaris cultures were not heat-active but were more psychrotolerant than Beauveria bassiana and Metarhizium robertsii isolates from the Palearctic temperate zone. Wax moth Galleria mellonella larvae was able to overcome C. militaris infection (injected hyphal bodies) in an active state (25 ?C), but mortality increased 5.5-fold during diapause induced by low temperature (15 ?C). Cold stupor (5 ?C) significantly prolonged the development of mycosis. If it was fought off at 25 ?C, the infection in larvae could be activated in pupae under cold conditions (8 ?C). Topical inoculation of larvae with ascospores was also more efficacious at 15 ?C than at 25 ?C. However, immunosuppression of larvae led to levelling of mortality at these temperatures. The study confirms the stenoecity of C. militaris and demonstrates crucial roles for the thermo-physiological state of insects in pathogenesis.} }