@ARTICLE{TreeBASE2Ref28966, author = {Eduardo de la Lastra and Maria Villarino and Juan Diego Astacio and Inmaculada Larena and Antonieta De Cal and Nieves Capote}, title = {Genetic diversity and vegetative compatibility of?Fusarium solani species complex of strawberry in Spain}, year = {2018}, keywords = {FSSC, Fusarium MLST, phylogenetic analysis, VCG, nursery, fruit production fields}, doi = {}, url = {http://}, pmid = {}, journal = {Phytopathology}, volume = {}, number = {}, pages = {}, abstract = {Fusarium solani is a soil-borne fungus that is pathogen on more than one hundred plants. It is the causal agent of crown and root rot in strawberry. We collected 101 F. solani isolates from diseased plants and soils from two distinct geographic areas of strawberry production in Spain: plant nurseries located in the North-Center of the country, and fruit production fields located in the South-West. Multilocus DNA sequences were constructed for the molecular identification of the isolates via Fusarium MLST online database, and to assess the phylogenetic relationships and diversity of the populations. In addition, the vegetative compatibility between isolates was analyzed based on complementation tests involving nitrate non-utilizing (nit) mutants. Phylogenetic analysis revealed that all strawberry-associated isolates were restricted to Clade 3 of FSSC. Forty-nine isolates from nursery population comprised of 4 distinct phylogenetic species i.e., FSSC 2 (F. keratoplasticum), FSSC 5 (F. solani sensu stricto), FSSC 9, and an unknown FSSC species, that matched with 5 vegetative compatibility groups (VCGs). Fifty-two isolates from fruit production fields comprised 5 strongly supported phylogenetic species i.e., FSSC 2, FSSC 3+4 (F. falciforme), FSSC 5, FSSC 6 (F. metavorans sp. nov.), and FSSC 11, distributed into 31 VCGs. Genetic and VCG variability of fruit production fields population was higher than those from nurseries. Genetic diversity analyses suggested that nurseries and fruit production fields are two different populations. The fact that some phylogenetic groups shared isolates from both populations, that heterocarion formation was detected between isolates from both populations, and that isolates from soils and diseased plants shared the same haplotype, suggests that infected runner plants and soils could be potential sources of inoculum of FSSC in fruit production fields.} }

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