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Citation for Study 934

About Citation title: "Estimating polyketide metabolic potential among non-sporulating fungal endophytes of Vaccinium macrocarpon.".
About This study was previously identified under the legacy study ID S812 (Status: Published).

Citation

Sauer M., Lu P., Sangari R., Kennedy S., Polishook J., Bills G., & An Z. 1992. Estimating polyketide metabolic potential among non-sporulating fungal endophytes of Vaccinium macrocarpon. Molecular Ecology, 106: 460-470.

Authors

  • Sauer M.
  • Lu P.
  • Sangari R.
  • Kennedy S.
  • Polishook J.
  • Bills G.
  • An Z.

Abstract

A set of 23 non-sporulating, unidentifiable endophytic fungi associated with wild Vaccinium macrocarpon were examined for their polyketide metabolite producing potential. Using two degenerate polymerase chain reaction primers (FPKSKSU-2 and FPKSKSD-1), we cloned and sequenced 12 ketosynthase domains from 11 of the 23 cranberry endophytic fungi. Phylogenetic analyses segregated the 12 ketosynthase domains into three groups. One group of four sequences was clustered with polyketide synthase genes involved in melanin formation. The second group of two ketosynthase sequences clustered with aflatoxin encoding fungal polyketide synthases. The remaining six ketosynthase domains were not clustered with any of the known fungal polyketide synthase groups. Of the 12 ketosynthase fragments, five contained one or more introns in the ~800 bp DNA region. In order to locate the phylogenetic origin of the polyketide synthase genes, phylogenetic relationships of the strains were inferred from small subunit ribosomal DNA sequences. Analyses of small subunit ribosomal DNA sequences showed that all but one of the strains grouped among the major clades of the ascomycetes. The exceptional strain, CR70, was probably an oomycete. Thirteen of the 22 ascomycetous fungi appeared within a clade that included Oidiodendron tenuissimum of the Myxotrichaceae.

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  • Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S934
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