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Citation for Study 14571

Citation title: "Combined morphological and molecular approach for identification of Stemphylium vesicarium inoculum in pear orchards ".

Study name: "Combined morphological and molecular approach for identification of Stemphylium vesicarium inoculum in pear orchards ".

This study is part of submission 14571 (Status: Published).

Citation

Puig M., Ruz L., Montesinos E., Moragrega C., & Llorente I. 2014. Combined morphological and molecular approach for identification of Stemphylium vesicarium inoculum in pear orchards. Fungal Biology, .

Authors

• Puig M.
• Ruz L.
• Montesinos E.
• Moragrega C.
• Llorente I.

Abstract

Stemphylium vesicarium is the causal agent of brown spot of pear (BSP), an important disease reported in pear-growing areas of Europe. The pathogen is able to colonize pear leaf debris and dead tissues of herbaceous plants on the ground and produce abundant ascospores and conidia that are capable of infecting pear trees. Inoculum monitoring in pear orchards is mainly achieved through spore traps and species identification is based on conidial morphology, but the similarities on conidial traits among species of Stemphylium make correct identification difficult. In this work a total of thirty-seven Stemphylium isolates recovered from different sources in pear orchards were characterized at the morphological, pathogenic and molecular level. Correspondence among ITS and gpd sequences and morphological traits were evaluated in order to determine their applicability in identification of S. vesicarium. Species identification based exclusively on morphological data was not feasible. Three different morphological groups were resolved according to colony and conidial morphology, but conidial dimensions of these groups were in the range described for S. vesicarium. Molecular analyses of the ITS and gpd sequences clustered field isolates in the S. vesicarium - S. herbarum-S. alfalfae- S. tomatonis and S. sedicola (84%), S. eturmiunum (13.5%), and S. gracilariae (2.5%) species groups. Combined morphological and molecular data were necessary for unambiguous identification of isolates in the S. vesicarium species group. Only isolates identified as S. vesicarium were pathogenic on pear. In this taxonomic group, differences in pathogenicity were observed, mainly related to their origin. Most isolates recovered from lesions (87%)were pathogenic, whereas 60% of isolates recovered from air samples or from nonhost plant species were nonpathogenic on pear. The study revealed that several species of Stemphylium coexist in pear orchards with S. vesicarium, the causal agent of BSP, and that combined morphological and molecular data are needed to differentiate them. Consequently, direct measurements of the airborne inoculum using volumetric spore traps and conidia may overestimate the actual pathogen population and its pathogenic potential.

Keywords

Brown spot of pear; gpd partial sequence; internal transcriber spacer; pathogenicity; sequencing; phylogeny

External links

• DOI: 10.1016/j.funbio.2014.11.006
• Other URL: http://www.sciencedirect.com/science/article/pii/S1878614614001779

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S14571
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref22267,
  	 author = {Mireia  Puig and Lídia  Ruz and Emilio  Montesinos and Concepció  Moragrega and Isidre  Llorente},
  	 title = {Combined morphological and molecular approach for identification of Stemphylium vesicarium inoculum in pear orchards },
  	 year = {2014},
  	 keywords = {Brown spot of pear; gpd partial sequence; internal transcriber spacer; pathogenicity; sequencing; phylogeny},
  	 doi = {10.1016/j.funbio.2014.11.006},
  	 url = {http://www.sciencedirect.com/science/article/pii/S1878614614001779},
  	 pmid = {},
  	 journal = {Fungal Biology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Stemphylium vesicarium is the causal agent of brown spot of pear (BSP), an important disease reported in pear-growing areas of Europe. The pathogen is able to colonize pear leaf debris and dead tissues of herbaceous plants on the ground and produce abundant
  ascospores and conidia that are capable of infecting pear trees. Inoculum monitoring in pear orchards is mainly achieved through spore traps and species identification is based on conidial morphology, but the similarities on conidial traits among species of Stemphylium make correct identification difficult. In this work a total of thirty-seven Stemphylium isolates recovered from different sources in pear orchards were characterized at the morphological, pathogenic and molecular level. Correspondence among ITS and gpd sequences and morphological traits were evaluated in order to determine their applicability in identification of S. vesicarium. Species identification based exclusively on morphological data was not feasible. Three different morphological groups were resolved according to colony and conidial morphology, but conidial dimensions of these groups were in the range described for S. vesicarium. Molecular analyses of the ITS and gpd sequences clustered field isolates in the S. vesicarium - S. herbarum-S. alfalfae- S. tomatonis and S. sedicola (84%), S. eturmiunum (13.5%), and S. gracilariae (2.5%) species groups. Combined morphological and molecular data were necessary for unambiguous identification of isolates in the S. vesicarium species group. Only isolates identified as S. vesicarium were pathogenic on pear. In this taxonomic group, differences in pathogenicity were observed, mainly related to their origin. Most isolates recovered from lesions (87%)were pathogenic, whereas 60% of isolates recovered from air samples or from nonhost plant species were nonpathogenic on pear. The study revealed that several species of Stemphylium coexist in pear orchards with S. vesicarium, the causal agent of BSP, and that combined morphological and molecular data are needed to differentiate them. Consequently, direct measurements of the airborne inoculum using volumetric spore traps and conidia may overestimate the actual pathogen population and its pathogenic potential.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 22267
  AU  - Puig,Mireia 
  AU  - Ruz,Lídia 
  AU  - Montesinos,Emilio 
  AU  - Moragrega,Concepció 
  AU  - Llorente,Isidre 
  T1  - Combined morphological and molecular approach for identification of Stemphylium vesicarium inoculum in pear orchards 
  PY  - 2014
  KW  - Brown spot of pear; gpd partial sequence; internal transcriber spacer; pathogenicity; sequencing; phylogeny
  UR  - http://www.sciencedirect.com/science/article/pii/S1878614614001779
  N2  - Stemphylium vesicarium is the causal agent of brown spot of pear (BSP), an important disease reported in pear-growing areas of Europe. The pathogen is able to colonize pear leaf debris and dead tissues of herbaceous plants on the ground and produce abundant
  ascospores and conidia that are capable of infecting pear trees. Inoculum monitoring in pear orchards is mainly achieved through spore traps and species identification is based on conidial morphology, but the similarities on conidial traits among species of Stemphylium make correct identification difficult. In this work a total of thirty-seven Stemphylium isolates recovered from different sources in pear orchards were characterized at the morphological, pathogenic and molecular level. Correspondence among ITS and gpd sequences and morphological traits were evaluated in order to determine their applicability in identification of S. vesicarium. Species identification based exclusively on morphological data was not feasible. Three different morphological groups were resolved according to colony and conidial morphology, but conidial dimensions of these groups were in the range described for S. vesicarium. Molecular analyses of the ITS and gpd sequences clustered field isolates in the S. vesicarium - S. herbarum-S. alfalfae- S. tomatonis and S. sedicola (84%), S. eturmiunum (13.5%), and S. gracilariae (2.5%) species groups. Combined morphological and molecular data were necessary for unambiguous identification of isolates in the S. vesicarium species group. Only isolates identified as S. vesicarium were pathogenic on pear. In this taxonomic group, differences in pathogenicity were observed, mainly related to their origin. Most isolates recovered from lesions (87%)were pathogenic, whereas 60% of isolates recovered from air samples or from nonhost plant species were nonpathogenic on pear. The study revealed that several species of Stemphylium coexist in pear orchards with S. vesicarium, the causal agent of BSP, and that combined morphological and molecular data are needed to differentiate them. Consequently, direct measurements of the airborne inoculum using volumetric spore traps and conidia may overestimate the actual pathogen population and its pathogenic potential.
  L3  - 10.1016/j.funbio.2014.11.006
  JF  - Fungal Biology
  VL  - 
  IS  - 
  ER  -