CiteULike

Delicious

Connotea

Citation for Study 12411

Citation title: "Perforin evolved from a gene duplication of MPEG1, followed by a complex pattern of gene gain and loss within Euteleostomi".

Study name: "Perforin evolved from a gene duplication of MPEG1, followed by a complex pattern of gene gain and loss within Euteleostomi".

This study is part of submission 12411 (Status: Published).

Citation

D'angelo M.E., Dunstone M.A., Whisstock J.C., Trapani J.A., & Bird P.I. 2012. Perforin evolved from a gene duplication of MPEG1, followed by a complex pattern of gene gain and loss within Euteleostomi. BMC Evolutionary Biology, 12(1).

Authors

• D'angelo M.E. (submitter)
• Dunstone M.A.
• Whisstock J.C.
• Trapani J.A.
• Bird P.I.

Abstract

Background: The pore-forming protein perforin is central to the granule-exocytosis pathway used by cytotoxic lymphocytes to kill abnormal cells. Although this mechanism of killing is conserved in bony vertebrates, cytotoxic cells are present in other chordates and invertebrates, and their cytotoxic mechanism has not been elucidated. In order to understand the evolution of this pathway, here we characterize the origins and evolution of perforin. Results: We identified orthologs and homologs of human perforin in all but one species analysed from Euteleostomi, and present evidence for an earlier ortholog in Gnathostomata but not in more primitive chordates. In placental mammals perforin is a single copy gene, but there are multiple perforin genes in all lineages predating marsupials, except birds. Our comparisons of these many-to-one homologs of human perforin show that they mainly arose from lineage-specific gene duplications in multiple taxa, suggesting acquisition of new roles or different modes of regulation. We also present evidence that perforin arose from duplication of the ancient MPEG1 gene, and that it shares a common ancestor with the functionally related complement proteins. Conclusions: The evolution of perforin in vertebrates involved a complex pattern of gene, as well as intron, gain and loss. The primordial perforin gene arose at least 500 million years ago, at around the time that the major histocompatibility complex-T cell receptor antigen recognition system was established. As it is absent from primitive chordates and invertebrates, cytotoxic cells from these lineages must possess a different effector molecule or cytotoxic mechanism.

Keywords

Perforin, MPEG1, Complement, Cellular Immunity

External links

• DOI: 10.1186/1471-2148-12-59
• PMID: 22551122
• Other URL: http://www.biomedcentral.com/1471-2148/12/59/abstract

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S12411
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref20466,
  	 author = {Michael Eric D'Angelo and Michelle A. Dunstone and James C. Whisstock and Joseph A. Trapani and Phillip I. Bird},
  	 title = {Perforin evolved from a gene duplication of MPEG1, followed by a complex pattern of gene gain and loss within Euteleostomi},
  	 year = {2012},
  	 keywords = {Perforin, MPEG1, Complement, Cellular Immunity},
  	 doi = {10.1186/1471-2148-12-59},
  	 url = {http://www.biomedcentral.com/1471-2148/12/59/abstract},
  	 pmid = {22551122},
  	 journal = {BMC Evolutionary Biology},
  	 volume = {12},
  	 number = {1},
  	 pages = {},
  	 abstract = {Background: The pore-forming protein perforin is central to the granule-exocytosis
  pathway used by cytotoxic lymphocytes to kill abnormal cells. Although this mechanism
  of killing is conserved in bony vertebrates, cytotoxic cells are present in other chordates
  and invertebrates, and their cytotoxic mechanism has not been elucidated. In order to
  understand the evolution of this pathway, here we characterize the origins and evolution
  of perforin.
  Results: We identified orthologs and homologs of human perforin in all but one species
  analysed from Euteleostomi, and present evidence for an earlier ortholog
  in Gnathostomata but not in more primitive chordates. In placental mammals perforin is a
  single copy gene, but there are multiple perforin genes in all lineages predating
  marsupials, except birds. Our comparisons of these many-to-one homologs of human
  perforin show that they mainly arose from lineage-specific gene duplications in multiple
  taxa, suggesting acquisition of new roles or different modes of regulation. We also
  present evidence that perforin arose from duplication of the ancient MPEG1 gene, and
  that it shares a common ancestor with the functionally related complement proteins.
  Conclusions: The evolution of perforin in vertebrates involved a complex pattern of
  gene, as well as intron, gain and loss. The primordial perforin gene arose at least 500
  million years ago, at around the time that the major histocompatibility complex-T cell
  receptor antigen recognition system was established. As it is absent from primitive
  chordates and invertebrates, cytotoxic cells from these lineages must possess a different
  effector molecule or cytotoxic mechanism.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 20466
  AU  - D'Angelo,Michael Eric
  AU  - Dunstone,Michelle A.
  AU  - Whisstock,James C.
  AU  - Trapani,Joseph A.
  AU  - Bird,Phillip I.
  T1  - Perforin evolved from a gene duplication of MPEG1, followed by a complex pattern of gene gain and loss within Euteleostomi
  PY  - 2012
  KW  - Perforin
  KW  - MPEG1
  KW  - Complement
  KW  - Cellular Immunity
  UR  - http://www.biomedcentral.com/1471-2148/12/59/abstract
  N2  - Background: The pore-forming protein perforin is central to the granule-exocytosis
  pathway used by cytotoxic lymphocytes to kill abnormal cells. Although this mechanism
  of killing is conserved in bony vertebrates, cytotoxic cells are present in other chordates
  and invertebrates, and their cytotoxic mechanism has not been elucidated. In order to
  understand the evolution of this pathway, here we characterize the origins and evolution
  of perforin.
  Results: We identified orthologs and homologs of human perforin in all but one species
  analysed from Euteleostomi, and present evidence for an earlier ortholog
  in Gnathostomata but not in more primitive chordates. In placental mammals perforin is a
  single copy gene, but there are multiple perforin genes in all lineages predating
  marsupials, except birds. Our comparisons of these many-to-one homologs of human
  perforin show that they mainly arose from lineage-specific gene duplications in multiple
  taxa, suggesting acquisition of new roles or different modes of regulation. We also
  present evidence that perforin arose from duplication of the ancient MPEG1 gene, and
  that it shares a common ancestor with the functionally related complement proteins.
  Conclusions: The evolution of perforin in vertebrates involved a complex pattern of
  gene, as well as intron, gain and loss. The primordial perforin gene arose at least 500
  million years ago, at around the time that the major histocompatibility complex-T cell
  receptor antigen recognition system was established. As it is absent from primitive
  chordates and invertebrates, cytotoxic cells from these lineages must possess a different
  effector molecule or cytotoxic mechanism.
  L3  - 10.1186/1471-2148-12-59
  JF  - BMC Evolutionary Biology
  VL  - 12
  IS  - 1
  ER  -