@ARTICLE{TreeBASE2Ref15172,
author = {C. Donovan Bailey and Colin E. Hughes and Stephen A. Harris},
title = {Using RAPDS to develop DNA sequence loci for species level phylogeny reconstruction: an example from Leucaena (Fabaceae).},
year = {2004},
keywords = {},
doi = {10.1600/036364404772973483},
url = {},
pmid = {},
journal = {Systematic Botany},
volume = {29},
number = {1},
pages = {4--14},
abstract = {Resolving phylogenies among closely related species remains a perplexing problem in plant systematics. All too often cpDNA and nrDNA ITS are insufficiently variable to provide desired resolution. In the present study, we have adopted a RAPD-based approach to develop sequence-characterized regions, from the nuclear genome, with levels of DNA sequence variation appropriate for resolving relationships within subclades of Leucaena (Fabaceae). RAPDs were used to amplify a set of seven Leucaena accessions. Equal length fragments amplified in two or more species were sequenced and compared. Specific primers were designed for aligned product sets displaying similar or greater levels of variation than have been found in previous ITS studies. Two regions whose DNA sequences provided greater resolution and bootstrap support than ITS or cpDNA RFLP restriction site data sets for the same sample of Leucaena species are discussed in detail.}
}
Citation for Study 1034
Citation title:
"Using RAPDS to develop DNA sequence loci for species level phylogeny reconstruction: an example from Leucaena (Fabaceae).".
This study was previously identified under the legacy study ID S926
(Status: Published).
Citation
Bailey C., Hughes C., & Harris S. 2004. Using RAPDS to develop DNA sequence loci for species level phylogeny reconstruction: an example from Leucaena (Fabaceae). Systematic Botany, 29(1): 4-14.
Authors
-
Bailey C.
-
Hughes C.
-
Harris S.
Abstract
Resolving phylogenies among closely related species remains a perplexing problem in plant systematics. All too often cpDNA and nrDNA ITS are insufficiently variable to provide desired resolution. In the present study, we have adopted a RAPD-based approach to develop sequence-characterized regions, from the nuclear genome, with levels of DNA sequence variation appropriate for resolving relationships within subclades of Leucaena (Fabaceae). RAPDs were used to amplify a set of seven Leucaena accessions. Equal length fragments amplified in two or more species were sequenced and compared. Specific primers were designed for aligned product sets displaying similar or greater levels of variation than have been found in previous ITS studies. Two regions whose DNA sequences provided greater resolution and bootstrap support than ITS or cpDNA RFLP restriction site data sets for the same sample of Leucaena species are discussed in detail.
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S1034
- Other versions:
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- Show BibTeX reference
@ARTICLE{TreeBASE2Ref15172,
author = {C. Donovan Bailey and Colin E. Hughes and Stephen A. Harris},
title = {Using RAPDS to develop DNA sequence loci for species level phylogeny reconstruction: an example from Leucaena (Fabaceae).},
year = {2004},
keywords = {},
doi = {10.1600/036364404772973483},
url = {},
pmid = {},
journal = {Systematic Botany},
volume = {29},
number = {1},
pages = {4--14},
abstract = {Resolving phylogenies among closely related species remains a perplexing problem in plant systematics. All too often cpDNA and nrDNA ITS are insufficiently variable to provide desired resolution. In the present study, we have adopted a RAPD-based approach to develop sequence-characterized regions, from the nuclear genome, with levels of DNA sequence variation appropriate for resolving relationships within subclades of Leucaena (Fabaceae). RAPDs were used to amplify a set of seven Leucaena accessions. Equal length fragments amplified in two or more species were sequenced and compared. Specific primers were designed for aligned product sets displaying similar or greater levels of variation than have been found in previous ITS studies. Two regions whose DNA sequences provided greater resolution and bootstrap support than ITS or cpDNA RFLP restriction site data sets for the same sample of Leucaena species are discussed in detail.}
}
- Show RIS reference
TY - JOUR
ID - 15172
AU - Bailey,C. Donovan
AU - Hughes,Colin E.
AU - Harris,Stephen A.
T1 - Using RAPDS to develop DNA sequence loci for species level phylogeny reconstruction: an example from Leucaena (Fabaceae).
PY - 2004
UR - http://dx.doi.org/10.1600/036364404772973483
N2 - Resolving phylogenies among closely related species remains a perplexing problem in plant systematics. All too often cpDNA and nrDNA ITS are insufficiently variable to provide desired resolution. In the present study, we have adopted a RAPD-based approach to develop sequence-characterized regions, from the nuclear genome, with levels of DNA sequence variation appropriate for resolving relationships within subclades of Leucaena (Fabaceae). RAPDs were used to amplify a set of seven Leucaena accessions. Equal length fragments amplified in two or more species were sequenced and compared. Specific primers were designed for aligned product sets displaying similar or greater levels of variation than have been found in previous ITS studies. Two regions whose DNA sequences provided greater resolution and bootstrap support than ITS or cpDNA RFLP restriction site data sets for the same sample of Leucaena species are discussed in detail.
L3 - 10.1600/036364404772973483
JF - Systematic Botany
VL - 29
IS - 1
SP - 4
EP - 14
ER -
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