@ARTICLE{TreeBASE2Ref19335,
author = {John D Hall and Karolina Fucikova and Chien Lo and Louise A. Lewis and Kenneth G. Karol},
title = {An assessment of proposed DNA barcodes in freshwater green algae.},
year = {2010},
keywords = {DNA barcode, green algae, Charophyceae, Chlorophyceae, Zygnematophyceae, 18S rDNA, cox1, ITS rDNA, rbcL, tufA, UPA},
doi = {},
url = {http://},
pmid = {},
journal = {Cryptogamie Algologie},
volume = {31},
number = {4},
pages = {},
abstract = {The development of DNA barcodes for green algae would aid in their accurate and rapid identification. This is particularly important for the green algae because in many genera structural characters are few or difficult to observe. In this study we test the utility of several DNA barcode loci in seven distantly related species groups of charophytes and chlorophytes. The ITS rDNA and cox1 markers were the most variable, although cox1 was least applicable because it was successfully amplified and sequenced in only one test genus. The ITS1 and ITS2 rDNA regions each amplified only a subset of the charophytes. Both rbcL and tufA were moderately variable, although tufA may pose challenges in some lineages where the gene is encoded in the nucleus. The 18S rDNA and UPA were the least variable loci tested. Of the markers tested, rbcL, ITS and tufA (in chlorophytes) are the most promising for use as DNA barcodes. However, none of the loci tested were ideal for use across all tested lineages of green algae because of taxon-specific applicability and because none of the markers could distinguish closely related species in all groups tested.}
}
Citation for Study 11032
Citation title:
"An assessment of proposed DNA barcodes in freshwater green algae.".
Study name:
"An assessment of proposed DNA barcodes in freshwater green algae.".
This study is part of submission 11022
(Status: Published).
Citation
Hall J.D., Fucikova K., Lo C., Lewis L., & Karol K. 2010. An assessment of proposed DNA barcodes in freshwater green algae. Cryptogamie Algologie, 31(4).
Authors
-
Hall J.D.
-
Fucikova K.
(submitter)
8608614178
-
Lo C.
-
Lewis L.
-
Karol K.
Abstract
The development of DNA barcodes for green algae would aid in their accurate and rapid identification. This is particularly important for the green algae because in many genera structural characters are few or difficult to observe. In this study we test the utility of several DNA barcode loci in seven distantly related species groups of charophytes and chlorophytes. The ITS rDNA and cox1 markers were the most variable, although cox1 was least applicable because it was successfully amplified and sequenced in only one test genus. The ITS1 and ITS2 rDNA regions each amplified only a subset of the charophytes. Both rbcL and tufA were moderately variable, although tufA may pose challenges in some lineages where the gene is encoded in the nucleus. The 18S rDNA and UPA were the least variable loci tested. Of the markers tested, rbcL, ITS and tufA (in chlorophytes) are the most promising for use as DNA barcodes. However, none of the loci tested were ideal for use across all tested lineages of green algae because of taxon-specific applicability and because none of the markers could distinguish closely related species in all groups tested.
Keywords
DNA barcode, green algae, Charophyceae, Chlorophyceae, Zygnematophyceae, 18S rDNA, cox1, ITS rDNA, rbcL, tufA, UPA
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S11032
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref19335,
author = {John D Hall and Karolina Fucikova and Chien Lo and Louise A. Lewis and Kenneth G. Karol},
title = {An assessment of proposed DNA barcodes in freshwater green algae.},
year = {2010},
keywords = {DNA barcode, green algae, Charophyceae, Chlorophyceae, Zygnematophyceae, 18S rDNA, cox1, ITS rDNA, rbcL, tufA, UPA},
doi = {},
url = {http://},
pmid = {},
journal = {Cryptogamie Algologie},
volume = {31},
number = {4},
pages = {},
abstract = {The development of DNA barcodes for green algae would aid in their accurate and rapid identification. This is particularly important for the green algae because in many genera structural characters are few or difficult to observe. In this study we test the utility of several DNA barcode loci in seven distantly related species groups of charophytes and chlorophytes. The ITS rDNA and cox1 markers were the most variable, although cox1 was least applicable because it was successfully amplified and sequenced in only one test genus. The ITS1 and ITS2 rDNA regions each amplified only a subset of the charophytes. Both rbcL and tufA were moderately variable, although tufA may pose challenges in some lineages where the gene is encoded in the nucleus. The 18S rDNA and UPA were the least variable loci tested. Of the markers tested, rbcL, ITS and tufA (in chlorophytes) are the most promising for use as DNA barcodes. However, none of the loci tested were ideal for use across all tested lineages of green algae because of taxon-specific applicability and because none of the markers could distinguish closely related species in all groups tested.}
}
- Show RIS reference
TY - JOUR
ID - 19335
AU - Hall,John D
AU - Fucikova,Karolina
AU - Lo,Chien
AU - Lewis,Louise A.
AU - Karol,Kenneth G.
T1 - An assessment of proposed DNA barcodes in freshwater green algae.
PY - 2010
KW - DNA barcode
KW - green algae
KW - Charophyceae
KW - Chlorophyceae
KW - Zygnematophyceae
KW - 18S rDNA
KW - cox1
KW - ITS rDNA
KW - rbcL
KW - tufA
KW - UPA
UR - http://dx.doi.org/
N2 - The development of DNA barcodes for green algae would aid in their accurate and rapid identification. This is particularly important for the green algae because in many genera structural characters are few or difficult to observe. In this study we test the utility of several DNA barcode loci in seven distantly related species groups of charophytes and chlorophytes. The ITS rDNA and cox1 markers were the most variable, although cox1 was least applicable because it was successfully amplified and sequenced in only one test genus. The ITS1 and ITS2 rDNA regions each amplified only a subset of the charophytes. Both rbcL and tufA were moderately variable, although tufA may pose challenges in some lineages where the gene is encoded in the nucleus. The 18S rDNA and UPA were the least variable loci tested. Of the markers tested, rbcL, ITS and tufA (in chlorophytes) are the most promising for use as DNA barcodes. However, none of the loci tested were ideal for use across all tested lineages of green algae because of taxon-specific applicability and because none of the markers could distinguish closely related species in all groups tested.
L3 -
JF - Cryptogamie Algologie
VL - 31
IS - 4
ER -
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