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Citation for Study 880

Citation title: "Two Erysiphe species associated with recent outbreak of soybean powdery mildew: results of molecular phylogenetic analysis based on nuclear rDNA sequences.".

This study was previously identified under the legacy study ID S746 (Status: Published).

Citation

Takamatsu S., Shin H., Paksiri U., Limkaisang S., Taguchi Y., Thi binh N., & Sato Y. 2002. Two Erysiphe species associated with recent outbreak of soybean powdery mildew: results of molecular phylogenetic analysis based on nuclear rDNA sequences. Mycoscience, 43(4): 333-341.

Authors

• Takamatsu S.
• Shin H.
• Paksiri U.
• Limkaisang S.
• Taguchi Y.
• Thi binh N.
• Sato Y.

Abstract

Serious outbreaks of powdery mildew by a fungus belonging to the mitosporic genus Oidium subgenus Pseudoidium have been reported on soybean (Glycine max) in a wide area of eastern Asia since 1998. The taxonomic and phylogenetic placement of the causal fungus has not yet been determined because of lack of the perfect stage. We found ascomata having mycelioid appendages on a single leaf of soybean infested by powdery mildew. Molecular phylogenetic analysis was conducted based on a total of 14 sequences of the rDNA internal transcribed spacer (ITS) region from 13 soybean and wild soybean (Glycine soja) materials collected in Japan, Korea, Vietnam, and the United States, combined with 47 sequence data obtained from the DNA databases. It was revealed that two Erysiphe species were associated with the outbreak of soybean powdery mildew. There was 16% difference between the two species in genetic divergence of the ITS sequence. One species with perfect stage has an ITS sequence identical to that of Erysiphe glycines on Amphicarpaea and is identified as Erysiphe glycines based on the ITS sequence and morphology of ascomata. The second species, without the perfect stage, is likely to be Erysiphe diffusa (= Microsphaera diffusa), known as the fungus causing soybean powdery mildew in the United States, because the ITS sequences are identical to those from materials collected in the United States. However, we need materials having ascomata of E. diffusa to confirm the species name.

Keywords

Erysiphaceae; Glycine max; ITS; Microsphaera; Oidium subgenus Pseudoidium

External links

• DOI: 10.1007/s102670200049

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S880
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref17766,
  	 author = {Susumu  Takamatsu and Hyeon-Dong  Shin and Urailak  Paksiri and Saranya  Limkaisang and Yusuke  Taguchi and N.  Thi Binh and Yukio  Sato},
  	 title = {Two Erysiphe species associated with recent outbreak of soybean powdery mildew: results of molecular phylogenetic analysis based on nuclear rDNA sequences.},
  	 year = {2002},
  	 keywords = {Erysiphaceae; Glycine max; ITS; Microsphaera; Oidium subgenus Pseudoidium},
  	 doi = {10.1007/s102670200049},
  	 url = {},
  	 pmid = {},
  	 journal = {Mycoscience},
  	 volume = {43},
  	 number = {4},
  	 pages = {333--341},
  	 abstract = {Serious outbreaks of powdery mildew by a fungus belonging to the mitosporic genus Oidium subgenus Pseudoidium have been reported on soybean (Glycine max) in a wide area of eastern Asia since 1998. The taxonomic and phylogenetic placement of the causal fungus has not yet been determined because of lack of the perfect stage. We found ascomata having mycelioid appendages on a single leaf of soybean infested by powdery mildew. Molecular phylogenetic analysis was conducted based on a total of 14 sequences of the rDNA internal transcribed spacer (ITS) region from 13 soybean and wild soybean (Glycine soja) materials collected in Japan, Korea, Vietnam, and the United States, combined with 47 sequence data obtained from the DNA databases. It was revealed that two Erysiphe species were associated with the outbreak of soybean powdery mildew. There was 16% difference between the two species in genetic divergence of the ITS sequence. One species with perfect stage has an ITS sequence identical to that of Erysiphe glycines on Amphicarpaea and is identified as Erysiphe glycines based on the ITS sequence and morphology of ascomata. The second species, without the perfect stage, is likely to be Erysiphe diffusa (= Microsphaera diffusa), known as the fungus causing soybean powdery mildew in the United States, because the ITS sequences are identical to those from materials collected in the United States. However, we need materials having ascomata of E. diffusa to confirm the species name.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 17766
  AU  - Takamatsu,Susumu 
  AU  - Shin,Hyeon-Dong 
  AU  - Paksiri,Urailak 
  AU  - Limkaisang,Saranya 
  AU  - Taguchi,Yusuke 
  AU  - Thi Binh,N. 
  AU  - Sato,Yukio 
  T1  - Two Erysiphe species associated with recent outbreak of soybean powdery mildew: results of molecular phylogenetic analysis based on nuclear rDNA sequences.
  PY  - 2002
  KW  - Erysiphaceae; Glycine max; ITS; Microsphaera; Oidium subgenus Pseudoidium
  UR  - http://dx.doi.org/10.1007/s102670200049
  N2  - Serious outbreaks of powdery mildew by a fungus belonging to the mitosporic genus Oidium subgenus Pseudoidium have been reported on soybean (Glycine max) in a wide area of eastern Asia since 1998. The taxonomic and phylogenetic placement of the causal fungus has not yet been determined because of lack of the perfect stage. We found ascomata having mycelioid appendages on a single leaf of soybean infested by powdery mildew. Molecular phylogenetic analysis was conducted based on a total of 14 sequences of the rDNA internal transcribed spacer (ITS) region from 13 soybean and wild soybean (Glycine soja) materials collected in Japan, Korea, Vietnam, and the United States, combined with 47 sequence data obtained from the DNA databases. It was revealed that two Erysiphe species were associated with the outbreak of soybean powdery mildew. There was 16% difference between the two species in genetic divergence of the ITS sequence. One species with perfect stage has an ITS sequence identical to that of Erysiphe glycines on Amphicarpaea and is identified as Erysiphe glycines based on the ITS sequence and morphology of ascomata. The second species, without the perfect stage, is likely to be Erysiphe diffusa (= Microsphaera diffusa), known as the fungus causing soybean powdery mildew in the United States, because the ITS sequences are identical to those from materials collected in the United States. However, we need materials having ascomata of E. diffusa to confirm the species name.
  L3  - 10.1007/s102670200049
  JF  - Mycoscience
  VL  - 43
  IS  - 4
  SP  - 333
  EP  - 341
  ER  -