@ARTICLE{TreeBASE2Ref26896,
author = {Nicole K. Reynolds and Matthew E. Smith and Eric Dennis Tretter and Justin Gause and Dustin Heeney and Mat?as Jorge Cafaro and James F. Smith and Steven J. Novak and William A. Bourland and Merlin M. White},
title = {Resolving relationships at the animal-fungal divergence: A molecular phylogenetic study of the protist trichomycetes (Ichthyosporea, Eccrinida)},
year = {2017},
keywords = {Eccrinida, evolution, Ichthyosporea, protists, symbiosis, trichomycetes},
doi = {10.1016/j.ympev.2017.02.007},
url = {http://},
pmid = {},
journal = {Molecular Phylogenetics and Evolution},
volume = {109},
number = {},
pages = {447--464},
abstract = {Trichomycetes is a group of microorganisms that was considered a class of fungi comprising four orders of commensal, gut-dwelling endosymbionts obligately associated with arthropods. Since molecular phylogenies revealed two of those orders (Amoebidiales and Eccrinales = ?protist trichos?) to be closely related to members of the protist class Ichthyosporea (= Mesomycetozoea), trichomycetes have been considered an ecological association of both early-diverging fungi and protists. Understanding of the taxonomy, evolution, and diversity of the protist trichos is lacking largely due to the difficulties inherent in species collection that have contributed to undersampling and understudy. The most recent classification divides the protist trichos between two families, Amoebidiidae and Eccrinidae (suborder Trichomycina, order Eccrinida). However, there is no comprehensive molecular phylogeny available for this group and major questions about the systematics of protist trichos remain unanswered. Therefore, we generated 18S and 28S rDNA sequences for 106 protist tricho samples and combined them with publicly available Eccrinida sequences for phylogenetic analyses. We also sequenced a conserved protein-coding gene (heat-shock 70 protein) to obtain a multigene data set. We conducted ancestral state reconstruction (ASR) and Bayesian tip-association significance test (BaTS) analyses by mapping six morphological and ecological characters onto the resulting phylogenetic trees. Our results demonstrate: 1) several ecological and morphological character states (habitat, host type, host stage at time of infestation, location within host, spore production, and growth form) are significantly correlated with the phylogeny, and 2) two additional protist tricho families should be incorporated into the taxonomy to reflect phylogenetic relationships. Our data suggest that an integrated strategy that combines morphological, ecological, and molecular characters is needed to further resolve and clarify the systematics of the Eccrinida.}
}
Citation for Study 20595
Citation title:
"Resolving relationships at the animal-fungal divergence: A molecular phylogenetic study of the protist trichomycetes (Ichthyosporea, Eccrinida)".
Study name:
"Resolving relationships at the animal-fungal divergence: A molecular phylogenetic study of the protist trichomycetes (Ichthyosporea, Eccrinida)".
This study is part of submission 20595
(Status: Published).
Citation
Reynolds N.K., Smith M.E., Tretter E.D., Gause J., Heeney D., Cafaro M.J., Smith J., Novak S.J., Bourland W.A., & White M.M. 2017. Resolving relationships at the animal-fungal divergence: A molecular phylogenetic study of the protist trichomycetes (Ichthyosporea, Eccrinida). Molecular Phylogenetics and Evolution, 109: 447-464.
Authors
-
Reynolds N.K.
(submitter)
-
Smith M.E.
-
Tretter E.D.
2089081220
-
Gause J.
-
Heeney D.
-
Cafaro M.J.
-
Smith J.
-
Novak S.J.
-
Bourland W.A.
-
White M.M.
Abstract
Trichomycetes is a group of microorganisms that was considered a class of fungi comprising four orders of commensal, gut-dwelling endosymbionts obligately associated with arthropods. Since molecular phylogenies revealed two of those orders (Amoebidiales and Eccrinales = ?protist trichos?) to be closely related to members of the protist class Ichthyosporea (= Mesomycetozoea), trichomycetes have been considered an ecological association of both early-diverging fungi and protists. Understanding of the taxonomy, evolution, and diversity of the protist trichos is lacking largely due to the difficulties inherent in species collection that have contributed to undersampling and understudy. The most recent classification divides the protist trichos between two families, Amoebidiidae and Eccrinidae (suborder Trichomycina, order Eccrinida). However, there is no comprehensive molecular phylogeny available for this group and major questions about the systematics of protist trichos remain unanswered. Therefore, we generated 18S and 28S rDNA sequences for 106 protist tricho samples and combined them with publicly available Eccrinida sequences for phylogenetic analyses. We also sequenced a conserved protein-coding gene (heat-shock 70 protein) to obtain a multigene data set. We conducted ancestral state reconstruction (ASR) and Bayesian tip-association significance test (BaTS) analyses by mapping six morphological and ecological characters onto the resulting phylogenetic trees. Our results demonstrate: 1) several ecological and morphological character states (habitat, host type, host stage at time of infestation, location within host, spore production, and growth form) are significantly correlated with the phylogeny, and 2) two additional protist tricho families should be incorporated into the taxonomy to reflect phylogenetic relationships. Our data suggest that an integrated strategy that combines morphological, ecological, and molecular characters is needed to further resolve and clarify the systematics of the Eccrinida.
Keywords
Eccrinida, evolution, Ichthyosporea, protists, symbiosis, trichomycetes
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S20595
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref26896,
author = {Nicole K. Reynolds and Matthew E. Smith and Eric Dennis Tretter and Justin Gause and Dustin Heeney and Mat?as Jorge Cafaro and James F. Smith and Steven J. Novak and William A. Bourland and Merlin M. White},
title = {Resolving relationships at the animal-fungal divergence: A molecular phylogenetic study of the protist trichomycetes (Ichthyosporea, Eccrinida)},
year = {2017},
keywords = {Eccrinida, evolution, Ichthyosporea, protists, symbiosis, trichomycetes},
doi = {10.1016/j.ympev.2017.02.007},
url = {http://},
pmid = {},
journal = {Molecular Phylogenetics and Evolution},
volume = {109},
number = {},
pages = {447--464},
abstract = {Trichomycetes is a group of microorganisms that was considered a class of fungi comprising four orders of commensal, gut-dwelling endosymbionts obligately associated with arthropods. Since molecular phylogenies revealed two of those orders (Amoebidiales and Eccrinales = ?protist trichos?) to be closely related to members of the protist class Ichthyosporea (= Mesomycetozoea), trichomycetes have been considered an ecological association of both early-diverging fungi and protists. Understanding of the taxonomy, evolution, and diversity of the protist trichos is lacking largely due to the difficulties inherent in species collection that have contributed to undersampling and understudy. The most recent classification divides the protist trichos between two families, Amoebidiidae and Eccrinidae (suborder Trichomycina, order Eccrinida). However, there is no comprehensive molecular phylogeny available for this group and major questions about the systematics of protist trichos remain unanswered. Therefore, we generated 18S and 28S rDNA sequences for 106 protist tricho samples and combined them with publicly available Eccrinida sequences for phylogenetic analyses. We also sequenced a conserved protein-coding gene (heat-shock 70 protein) to obtain a multigene data set. We conducted ancestral state reconstruction (ASR) and Bayesian tip-association significance test (BaTS) analyses by mapping six morphological and ecological characters onto the resulting phylogenetic trees. Our results demonstrate: 1) several ecological and morphological character states (habitat, host type, host stage at time of infestation, location within host, spore production, and growth form) are significantly correlated with the phylogeny, and 2) two additional protist tricho families should be incorporated into the taxonomy to reflect phylogenetic relationships. Our data suggest that an integrated strategy that combines morphological, ecological, and molecular characters is needed to further resolve and clarify the systematics of the Eccrinida.}
}
- Show RIS reference
TY - JOUR
ID - 26896
AU - Reynolds,Nicole K.
AU - Smith,Matthew E.
AU - Tretter,Eric Dennis
AU - Gause,Justin
AU - Heeney,Dustin
AU - Cafaro,Mat?as Jorge
AU - Smith,James F.
AU - Novak,Steven J.
AU - Bourland,William A.
AU - White,Merlin M.
T1 - Resolving relationships at the animal-fungal divergence: A molecular phylogenetic study of the protist trichomycetes (Ichthyosporea, Eccrinida)
PY - 2017
KW - Eccrinida
KW - evolution
KW - Ichthyosporea
KW - protists
KW - symbiosis
KW - trichomycetes
UR - http://dx.doi.org/10.1016/j.ympev.2017.02.007
N2 - Trichomycetes is a group of microorganisms that was considered a class of fungi comprising four orders of commensal, gut-dwelling endosymbionts obligately associated with arthropods. Since molecular phylogenies revealed two of those orders (Amoebidiales and Eccrinales = ?protist trichos?) to be closely related to members of the protist class Ichthyosporea (= Mesomycetozoea), trichomycetes have been considered an ecological association of both early-diverging fungi and protists. Understanding of the taxonomy, evolution, and diversity of the protist trichos is lacking largely due to the difficulties inherent in species collection that have contributed to undersampling and understudy. The most recent classification divides the protist trichos between two families, Amoebidiidae and Eccrinidae (suborder Trichomycina, order Eccrinida). However, there is no comprehensive molecular phylogeny available for this group and major questions about the systematics of protist trichos remain unanswered. Therefore, we generated 18S and 28S rDNA sequences for 106 protist tricho samples and combined them with publicly available Eccrinida sequences for phylogenetic analyses. We also sequenced a conserved protein-coding gene (heat-shock 70 protein) to obtain a multigene data set. We conducted ancestral state reconstruction (ASR) and Bayesian tip-association significance test (BaTS) analyses by mapping six morphological and ecological characters onto the resulting phylogenetic trees. Our results demonstrate: 1) several ecological and morphological character states (habitat, host type, host stage at time of infestation, location within host, spore production, and growth form) are significantly correlated with the phylogeny, and 2) two additional protist tricho families should be incorporated into the taxonomy to reflect phylogenetic relationships. Our data suggest that an integrated strategy that combines morphological, ecological, and molecular characters is needed to further resolve and clarify the systematics of the Eccrinida.
L3 - 10.1016/j.ympev.2017.02.007
JF - Molecular Phylogenetics and Evolution
VL - 109
IS -
SP - 447
EP - 464
ER -