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Citation for Study 11586

Citation title: "Detection of parasitizing coccidia and determination of host crane species, sex and genotype by faecal DNA analysis".

Study name: "Detection of parasitizing coccidia and determination of host crane species, sex and genotype by faecal DNA analysis".

This study is part of submission 11576 (Status: Published).

Citation

Honma H., Suyama Y., & Nakai Y. 2011. Detection of parasitizing coccidia and determination of host crane species, sex and genotype by faecal DNA analysis. Molecular Ecology Resources, .

Authors

• Honma H. (submitter) +81(6)6879-8279
• Suyama Y.
• Nakai Y.

Abstract

In Japan, the three main crane species are the endangered red-crowned crane (Grus japonensis) inhabiting Hokkaido, the northernmost island of Japan; the vulnerable hooded crane (G. monacha); and the vulnerable white-naped crane (G. vipio). Both the hooded and white-naped cranes migrate in winter to Izumi in Kyushu, the southern island of Japan. In this study, we investigated the cranes and their coccidian parasites, through a targeted molecular approach using faecal DNA to develop a non-invasive method for infectious disease research. To determine the origin of non-invasively collected faecal samples, host species were identified by sequencing a region of approximately 470 bp of the mitochondrial 16S ribosomal RNA gene in the faecal DNA. Furthermore, to avoid sample redundancy, individual determination was performed by fragment analysis using microsatellite and sex-linked markers. For microsatellite genotyping, previously reported markers and markers isolated in this study were examined, and seven loci for red-crowned cranes, eight for hooded cranes and six for white-naped cranes displayed polymorphisms. A low error rate was demonstrated by comparing microsatellite data generated from faecal DNA samples with that generated from feather DNA samples, indicating a high reliability. Polymerase chain reaction-based capillary electrophoresis (PCR-CE), employing genetic markers in the second internal transcribed spacer (ITS2) of nuclear ribosomal DNA, was employed to detect crane coccidia. The sensitivity of detection of PCR-CE using faecal DNA was inferior to that with traditional microscopy; however, our results suggest that PCR-CE can depict crane coccidia diversity with higher resolution and it is a useful tool to characterize community composition of coccidia in detail.

Keywords

red-crowned crane, hooded crane, white-naped crane, species and sex determination, microsatellite, Eimeria

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S11586
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref19768,
  	 author = {Hajime  Honma and Yoshihisa  Suyama and Yutaka  Nakai},
  	 title = {Detection of parasitizing coccidia and determination of host crane species, sex and genotype by faecal DNA analysis},
  	 year = {2011},
  	 keywords = {red-crowned crane, hooded crane, white-naped crane, species and sex determination, microsatellite, Eimeria},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Molecular Ecology Resources},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {In Japan, the three main crane species are the endangered red-crowned crane (Grus japonensis) inhabiting Hokkaido, the northernmost island of Japan; the vulnerable hooded crane (G. monacha); and the vulnerable white-naped crane (G. vipio). Both the hooded and white-naped cranes migrate in winter to Izumi in Kyushu, the southern island of Japan. In this study, we investigated the cranes and their coccidian parasites, through a targeted molecular approach using faecal DNA to develop a non-invasive method for infectious disease research. To determine the origin of non-invasively collected faecal samples, host species were identified by sequencing a region of approximately 470 bp of the mitochondrial 16S ribosomal RNA gene in the faecal DNA. Furthermore, to avoid sample redundancy, individual determination was performed by fragment analysis using microsatellite and sex-linked markers. For microsatellite genotyping, previously reported markers and markers isolated in this study were examined, and seven loci for red-crowned cranes, eight for hooded cranes and six for white-naped cranes displayed polymorphisms. A low error rate was demonstrated by comparing microsatellite data generated from faecal DNA samples with that generated from feather DNA samples, indicating a high reliability. Polymerase chain reaction-based capillary electrophoresis (PCR-CE), employing genetic markers in the second internal transcribed spacer (ITS2) of nuclear ribosomal DNA, was employed to detect crane coccidia. The sensitivity of detection of PCR-CE using faecal DNA was inferior to that with traditional microscopy; however, our results suggest that PCR-CE can depict crane coccidia diversity with higher resolution and it is a useful tool to characterize community composition of coccidia in detail.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 19768
  AU  - Honma,Hajime 
  AU  - Suyama,Yoshihisa 
  AU  - Nakai,Yutaka 
  T1  - Detection of parasitizing coccidia and determination of host crane species, sex and genotype by faecal DNA analysis
  PY  - 2011
  KW  - red-crowned crane
  KW  - hooded crane
  KW  - white-naped crane
  KW  - species and sex determination
  KW  - microsatellite
  KW  - Eimeria
  UR  - http://dx.doi.org/
  N2  - In Japan, the three main crane species are the endangered red-crowned crane (Grus japonensis) inhabiting Hokkaido, the northernmost island of Japan; the vulnerable hooded crane (G. monacha); and the vulnerable white-naped crane (G. vipio). Both the hooded and white-naped cranes migrate in winter to Izumi in Kyushu, the southern island of Japan. In this study, we investigated the cranes and their coccidian parasites, through a targeted molecular approach using faecal DNA to develop a non-invasive method for infectious disease research. To determine the origin of non-invasively collected faecal samples, host species were identified by sequencing a region of approximately 470 bp of the mitochondrial 16S ribosomal RNA gene in the faecal DNA. Furthermore, to avoid sample redundancy, individual determination was performed by fragment analysis using microsatellite and sex-linked markers. For microsatellite genotyping, previously reported markers and markers isolated in this study were examined, and seven loci for red-crowned cranes, eight for hooded cranes and six for white-naped cranes displayed polymorphisms. A low error rate was demonstrated by comparing microsatellite data generated from faecal DNA samples with that generated from feather DNA samples, indicating a high reliability. Polymerase chain reaction-based capillary electrophoresis (PCR-CE), employing genetic markers in the second internal transcribed spacer (ITS2) of nuclear ribosomal DNA, was employed to detect crane coccidia. The sensitivity of detection of PCR-CE using faecal DNA was inferior to that with traditional microscopy; however, our results suggest that PCR-CE can depict crane coccidia diversity with higher resolution and it is a useful tool to characterize community composition of coccidia in detail.
  L3  - 
  JF  - Molecular Ecology Resources
  VL  - 
  IS  - 
  ER  -