CiteULike

Delicious

Connotea

Citation for Study 14407

Citation title: "Comparative analysis of two emerging rice seed pathogens".

Study name: "Comparative analysis of two emerging rice seed pathogens".

This study is part of submission 14407 (Status: Published).

Citation

Fory P., Triplett L.R., Ballen C., Duitama J., Aricapa M., Abello J., Prado G., Correa F., Hamilton J., Leach J., Tohme J., & Mosquera G. 2013. Comparative analysis of two emerging rice seed pathogens. Phytopathology, .

Authors

• Fory P.
• Triplett L.R. (submitter) 574-709-8701
• Ballen C.
• Duitama J.
• Aricapa M.
• Abello J.
• Prado G.
• Correa F.
• Hamilton J.
• Leach J.
• Tohme J.
• Mosquera G.

Abstract

Seed sterility and grain discoloration limit rice production in Colombia and several Central American countries. In samples of discolored rice seed grown in Colombian fields, two rice pathogenic bacterial species were isolated; Burkholderia glumae and B. gladioli, and several of the field isolates were compared phenotypically. An artificial inoculation assay was developed and used to determine that while both bacterial species can colonize and cause symptoms on rice grains, B. glumae causes higher levels of grain sterility than B. gladioli isolates. B. glumae reduced rice yield up to 75%, while B. gladioli did not affect yield. Therefore, while the two species are closely related and inhabit a similar niche, B. glumae is the more aggressive and damaging pathogen of rice. To identify potential sources of virulence differences between B. glumae and B. gladioli, four previously sequenced genomes of Asian and US strains of B. glumae and B. gladioli were compared with each other and with two draft genomes of Colombian B. glumae and B. gladioli isolates generated for this study. While characterized Burkholderia virulence factors involved in toxin and lipase production and quorum sensing are highly conserved between the two species, B. glumae and B. gladioli strains are predicted to encode distinct complements of type VI secretion systems, transcriptional regulators, membrane sensing proteins, and type III secreted effectors. These findings show that both B. glumae and B. gladioli can threaten grain quality although only one species affects yield, and identifies differences in the core genetic makeup of the species that could contribute to the phenotypic differences between them.

Keywords

Burkholderia glumae, Burkholderia gladioli, bacterial panicle blight

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S14407
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref22137,
  	 author = {Paola  Fory and Lindsay R Triplett and Carolina  Ballen and Jorge  duitama and Maria  Aricapa and J  Abello and Gustavo  Prado and Fernando  Correa and John  Hamilton and jan  leach and Joseph  Tohme and Gloria  Mosquera},
  	 title = {Comparative analysis of two emerging rice seed pathogens},
  	 year = {2013},
  	 keywords = {Burkholderia glumae, Burkholderia gladioli, bacterial panicle blight},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Phytopathology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Seed sterility and grain discoloration limit rice production in Colombia and several Central American countries. In samples of discolored rice seed grown in Colombian fields, two rice pathogenic bacterial species were isolated; Burkholderia glumae and B. gladioli, and several of the field isolates were compared phenotypically. An artificial inoculation assay was developed and used to determine that while both bacterial species can colonize and cause symptoms on rice grains, B. glumae causes higher levels of grain sterility than B. gladioli isolates. B. glumae reduced rice yield up to 75%, while B. gladioli did not affect yield. Therefore, while the two species are closely related and inhabit a similar niche, B. glumae is the more aggressive and damaging pathogen of rice. To identify potential sources of virulence differences between B. glumae and B. gladioli, four previously sequenced genomes of Asian and US strains of B. glumae and B. gladioli were compared with each other and with two draft genomes of Colombian B. glumae and B. gladioli isolates generated for this study. While characterized Burkholderia virulence factors involved in toxin and lipase production and quorum sensing are highly conserved between the two species, B. glumae and B. gladioli strains are predicted to encode distinct complements of type VI secretion systems, transcriptional regulators, membrane sensing proteins, and type III secreted effectors. These findings show that both B. glumae and B. gladioli can threaten grain quality although only one species affects yield, and identifies differences in the core genetic makeup of the species that could contribute to the phenotypic differences between them. }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 22137
  AU  - Fory,Paola 
  AU  - Triplett,Lindsay R
  AU  - Ballen,Carolina 
  AU  - duitama,Jorge 
  AU  - Aricapa,Maria 
  AU  - Abello,J 
  AU  - Prado,Gustavo 
  AU  - Correa,Fernando 
  AU  - Hamilton,John 
  AU  - leach,jan 
  AU  - Tohme,Joseph 
  AU  - Mosquera,Gloria 
  T1  - Comparative analysis of two emerging rice seed pathogens
  PY  - 2013
  KW  - Burkholderia glumae
  KW  - Burkholderia gladioli
  KW  - bacterial panicle blight
  UR  - http://dx.doi.org/
  N2  - Seed sterility and grain discoloration limit rice production in Colombia and several Central American countries. In samples of discolored rice seed grown in Colombian fields, two rice pathogenic bacterial species were isolated; Burkholderia glumae and B. gladioli, and several of the field isolates were compared phenotypically. An artificial inoculation assay was developed and used to determine that while both bacterial species can colonize and cause symptoms on rice grains, B. glumae causes higher levels of grain sterility than B. gladioli isolates. B. glumae reduced rice yield up to 75%, while B. gladioli did not affect yield. Therefore, while the two species are closely related and inhabit a similar niche, B. glumae is the more aggressive and damaging pathogen of rice. To identify potential sources of virulence differences between B. glumae and B. gladioli, four previously sequenced genomes of Asian and US strains of B. glumae and B. gladioli were compared with each other and with two draft genomes of Colombian B. glumae and B. gladioli isolates generated for this study. While characterized Burkholderia virulence factors involved in toxin and lipase production and quorum sensing are highly conserved between the two species, B. glumae and B. gladioli strains are predicted to encode distinct complements of type VI secretion systems, transcriptional regulators, membrane sensing proteins, and type III secreted effectors. These findings show that both B. glumae and B. gladioli can threaten grain quality although only one species affects yield, and identifies differences in the core genetic makeup of the species that could contribute to the phenotypic differences between them. 
  L3  - 
  JF  - Phytopathology
  VL  - 
  IS  - 
  ER  -