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Citation for Study 2165

Citation title: "Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida.".

This study was previously identified under the legacy study ID S2171 (Status: Published).

Citation

Arauz F., Barquero M., Mackenzie S., Peres N.A., Timmer L., Mackenzie S., Peres N.A., Barquero M., Arauz L., & Timmer L. 2008. Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida. Phytopathology, null.

Authors

• Arauz F.
• Barquero M.
• Mackenzie S.
• Peres N.A.
• Timmer L.
• Mackenzie S.
• Peres N.A.
• Barquero M.
• Arauz L.
• Timmer L.

Abstract

Isolates of C. acutatum were collected from anthracnose-affected strawberry, leatherleaf fern, and Key lime; ripe rot-affected blueberry; and postbloom fruit drop (PFD)-affected sweet orange in Florida. Additional isolates from ripe rot-affected blueberry were collected from Georgia and North Carolina and from anthracnose-affected leatherleaf fern in Costa Rica. Based on sequence data from the ITS1-5.8S rRNA-ITS2 region of the rDNA repeat (ITS), the glutaraldehyde-3-phosphate dehydrogenase intron 2 (G3PD), and the glutamine synthase intron 2 (GS), isolates from the same host were identical or very similar to each other and distinct from those isolated from other hosts. Isolates from leatherleaf fern in Florida were the only exception. Among these isolates, there were two distinct G3PD and GS sequences that occurred in three of four possible combinations. Only one of these combinations occurred in Costa Rica. Although maximum parsimony trees constructed from genomic regions individually displayed little or no homoplasy, there was a lack of concordance among genealogies that was consistent with a history of recombination. This lack of concordance was particularly evident within a clade containing PFD, Key lime and leatherleaf fern isolates. Pathogenicity tests on blueberry and strawberry fruit; foliage of Key lime, leatherleaf fern, and strawberry; and citrus flowers showed that all isolates were highly pathogenic to their host of origin. Isolates were not pathogenic on foliage of heterologous hosts, however, several nonhomologous isolates were mildly or moderately pathogenic to citrus flowers and blueberry isolates were pathogenic to strawberry fruit. Overall, the data indicated that it is unlikely a pathogenic strain from one of the hosts examined would move to another of these hosts and produce a disease epidemic. There was also evidence that new strains may be produced by recombination among isolates for which no sexual stage has ever been observed.

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S2165
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref2102,
  	 author = {Felipe  Arauz and Marcia  Barquero and Steven  MacKenzie and Natalia A. Peres and L.W. (Pete)  Timmer and S. J.  MacKenzie and Natalia A. Peres and M. P.  Barquero and L. F.  Arauz and L. W.  Timmer},
  	 title = {Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida.},
  	 year = {2008},
  	 keywords = {},
  	 doi = {},
  	 url = {},
  	 pmid = {},
  	 journal = {Phytopathology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Isolates of C. acutatum were collected from anthracnose-affected strawberry, leatherleaf fern, and Key lime; ripe rot-affected blueberry; and postbloom fruit drop (PFD)-affected sweet orange in Florida. Additional isolates from ripe rot-affected blueberry were collected from Georgia and North Carolina and from anthracnose-affected leatherleaf fern in Costa Rica. Based on sequence data from the ITS1-5.8S rRNA-ITS2 region of the rDNA repeat (ITS), the glutaraldehyde-3-phosphate dehydrogenase intron 2 (G3PD), and the glutamine synthase intron 2 (GS), isolates from the same host were identical or very similar to each other and distinct from those isolated from other hosts. Isolates from leatherleaf fern in Florida were the only exception. Among these isolates, there were two distinct G3PD and GS sequences that occurred in three of four possible combinations. Only one of these combinations occurred in Costa Rica. Although maximum parsimony trees constructed from genomic regions individually displayed little or no homoplasy, there was a lack of concordance among genealogies that was consistent with a history of recombination. This lack of concordance was particularly evident within a clade containing PFD, Key lime and leatherleaf fern isolates. Pathogenicity tests on blueberry and strawberry fruit; foliage of Key lime, leatherleaf fern, and strawberry; and citrus flowers showed that all isolates were highly pathogenic to their host of origin. Isolates were not pathogenic on foliage of heterologous hosts, however, several nonhomologous isolates were mildly or moderately pathogenic to citrus flowers and blueberry isolates were pathogenic to strawberry fruit. Overall, the data indicated that it is unlikely a pathogenic strain from one of the hosts examined would move to another of these hosts and produce a disease epidemic. There was also evidence that new strains may be produced by recombination among isolates for which no sexual stage has ever been observed.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 2102
  AU  - Arauz,Felipe 
  AU  - Barquero,Marcia 
  AU  - MacKenzie,Steven 
  AU  - Peres,Natalia A.
  AU  - Timmer,L.W. (Pete) 
  AU  - MacKenzie,S. J. 
  AU  - Peres,Natalia A.
  AU  - Barquero,M. P. 
  AU  - Arauz,L. F. 
  AU  - Timmer,L. W. 
  T1  - Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida.
  PY  - 2008
  KW  - 
  UR  - 
  N2  - Isolates of C. acutatum were collected from anthracnose-affected strawberry, leatherleaf fern, and Key lime; ripe rot-affected blueberry; and postbloom fruit drop (PFD)-affected sweet orange in Florida. Additional isolates from ripe rot-affected blueberry were collected from Georgia and North Carolina and from anthracnose-affected leatherleaf fern in Costa Rica. Based on sequence data from the ITS1-5.8S rRNA-ITS2 region of the rDNA repeat (ITS), the glutaraldehyde-3-phosphate dehydrogenase intron 2 (G3PD), and the glutamine synthase intron 2 (GS), isolates from the same host were identical or very similar to each other and distinct from those isolated from other hosts. Isolates from leatherleaf fern in Florida were the only exception. Among these isolates, there were two distinct G3PD and GS sequences that occurred in three of four possible combinations. Only one of these combinations occurred in Costa Rica. Although maximum parsimony trees constructed from genomic regions individually displayed little or no homoplasy, there was a lack of concordance among genealogies that was consistent with a history of recombination. This lack of concordance was particularly evident within a clade containing PFD, Key lime and leatherleaf fern isolates. Pathogenicity tests on blueberry and strawberry fruit; foliage of Key lime, leatherleaf fern, and strawberry; and citrus flowers showed that all isolates were highly pathogenic to their host of origin. Isolates were not pathogenic on foliage of heterologous hosts, however, several nonhomologous isolates were mildly or moderately pathogenic to citrus flowers and blueberry isolates were pathogenic to strawberry fruit. Overall, the data indicated that it is unlikely a pathogenic strain from one of the hosts examined would move to another of these hosts and produce a disease epidemic. There was also evidence that new strains may be produced by recombination among isolates for which no sexual stage has ever been observed.
  L3  - 
  JF  - Phytopathology
  VL  - 
  IS  - 
  ER  -