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Citation for Study 13718

Citation title: "Development of molecular detection and field investigation for two citrus viroids in Taiwan".

Study name: "Development of molecular detection and field investigation for two citrus viroids in Taiwan".

This study is part of submission 13718 (Status: Published).

Citation

Lin C. 2012. Development of molecular detection and field investigation for two citrus viroids in Taiwan. European Journal of Plant Pathology, .

Authors

Lin C. (submitter) +88623625419

Abstract

Viroids cause diseases in citrus and have significant impact on the citrus industry. Two citrus viroids, CEVd (Citrus exocortis viroid) and HSVd (Hop stunt viroid) had been reported in Taiwan; however, the detection of these viroids still largely depends on bioassay and their prevalence and measurement for disease management in citrus remains to be investigated in Taiwan. In this study, we first collected samples from the field and used bioassay for viroid characterization, and also used primers (CEVd F1/R1 and HSVd F1/R1) reported by Bernad and Duran-Vila (2006) for RT-PCR detection. We were able to detect HSVd, but we were not able to detect CEVd in all collected samples. Thus, primer pairs, CEVd F194/R3, for successful detection of CEVd for Taiwanese isolates were developed. Additionally, we also developed multiplex RT-PCR for simultaneously detecting CEVd and HSVd using one-step RT-PCR. Real-time RT-PCR was also developed for more sensitive detection of CEVd and HSVd. Our field detection data demonstrated that although HSVd was only recently reported in Taiwan, it is more prevalent (30%) than CEVd (27.8%) and it is common to detect both CEVd and HSVd simultaneously in a citrus plant (18%).

Keywords

Citrus exocortis viroid ? Hop stunt viroid ? multiplex RT-PCR ? real-time RT-PCR ? co-infection ? redesigned primers

External links

About this resource

Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S13718
Other versions: Nexus NeXML

Show BibTeX reference

		@ARTICLE{TreeBASE2Ref21600,
	 author = {Chun-Yi  Lin},
	 title = {Development of molecular detection and field investigation for two citrus viroids in Taiwan},
	 year = {2012},
	 keywords = {Citrus exocortis viroid ? Hop stunt viroid ? multiplex RT-PCR ? real-time RT-PCR ? co-infection ? redesigned primers},
	 doi = {},
	 url = {http://},
	 pmid = {},
	 journal = {European Journal of Plant Pathology},
	 volume = {},
	 number = {},
	 pages = {},
	 abstract = {Viroids cause diseases in citrus and have significant impact on the citrus industry. Two citrus viroids, CEVd (Citrus exocortis viroid) and HSVd (Hop stunt viroid) had been reported in Taiwan; however, the detection of these viroids still largely depends on bioassay and their prevalence and measurement for disease management in citrus remains to be investigated in Taiwan. In this study, we first collected samples from the field and used bioassay for viroid characterization, and also used primers (CEVd F1/R1 and HSVd F1/R1) reported by Bernad and Duran-Vila (2006) for RT-PCR detection. We were able to detect HSVd, but we were not able to detect CEVd in all collected samples. Thus, primer pairs, CEVd F194/R3, for successful detection of CEVd for Taiwanese isolates were developed. Additionally, we also developed multiplex RT-PCR for simultaneously detecting CEVd and HSVd using one-step RT-PCR. Real-time RT-PCR was also developed for more sensitive detection of CEVd and HSVd. Our field detection data demonstrated that although HSVd was only recently reported in Taiwan, it is more prevalent (30%) than CEVd (27.8%) and it is common to detect both CEVd and HSVd simultaneously in a citrus plant (18%).}
}

Show RIS reference

TY - JOUR
ID - 21600
AU - Lin,Chun-Yi
T1 - Development of molecular detection and field investigation for two citrus viroids in Taiwan
PY - 2012
KW - Citrus exocortis viroid ? Hop stunt viroid ? multiplex RT-PCR ? real-time RT-PCR ? co-infection ? redesigned primers
UR - http://dx.doi.org/
N2 - Viroids cause diseases in citrus and have significant impact on the citrus industry. Two citrus viroids, CEVd (Citrus exocortis viroid) and HSVd (Hop stunt viroid) had been reported in Taiwan; however, the detection of these viroids still largely depends on bioassay and their prevalence and measurement for disease management in citrus remains to be investigated in Taiwan. In this study, we first collected samples from the field and used bioassay for viroid characterization, and also used primers (CEVd F1/R1 and HSVd F1/R1) reported by Bernad and Duran-Vila (2006) for RT-PCR detection. We were able to detect HSVd, but we were not able to detect CEVd in all collected samples. Thus, primer pairs, CEVd F194/R3, for successful detection of CEVd for Taiwanese isolates were developed. Additionally, we also developed multiplex RT-PCR for simultaneously detecting CEVd and HSVd using one-step RT-PCR. Real-time RT-PCR was also developed for more sensitive detection of CEVd and HSVd. Our field detection data demonstrated that although HSVd was only recently reported in Taiwan, it is more prevalent (30%) than CEVd (27.8%) and it is common to detect both CEVd and HSVd simultaneously in a citrus plant (18%).
L3 -
JF - European Journal of Plant Pathology
VL -
IS -
ER -