@ARTICLE{TreeBASE2Ref25047,
author = {Antoinette Alouch and michael joseph jowers and Paul G mungai and Samer Alasaad and Moses Otiende},
title = {First molecular identification of Pilobolus fungi from Africa (Kenya)},
year = {2015},
keywords = {Pilobolus, fungi, ITS, Phylogeny, Kenya},
doi = {},
url = {http://},
pmid = {},
journal = {Mycoscience},
volume = {},
number = {},
pages = {},
abstract = {Pilobolus fungi are abundant in herbivore dung. Species identification within the genus has relied on morphological data, which lacks accuracy due to the overlap of several characters. In this study we applied molecular identification to assess the presence of Pilobolus species. This method is more reliable than the previously used morphometric analyses and the generated sequences can be used for future work. Dung samples were collected from different herbivores in Nairobi National Park and sporangium isolates were obtained for pure cultures. DNA was extracted from the pure cultures and Internal Transcribed Spacer regions amplified and sequenced. Two species of Pilobolus were identified, Pilobolus pullus, with a high genetic affinity, and a second cryptic species, morphologically identified as P. crystallinus, matched Pilobolus heterosporus. Lack of identification of other Pilobolus species, known as key species in lungworm transmission, may suggest the absence of such in the sampled area. }
}
Citation for Study 18210
Citation title:
"First molecular identification of Pilobolus fungi from Africa (Kenya)".
Study name:
"First molecular identification of Pilobolus fungi from Africa (Kenya)".
This study is part of submission 18210
(Status: Published).
Citation
Alouch A., Jowers M.J., Mungai P.G., Alasaad S., & Otiende M. 2015. First molecular identification of Pilobolus fungi from Africa (Kenya). Mycoscience, .
Authors
-
Alouch A.
-
Jowers M.J.
(submitter)
0034633337281
-
Mungai P.G.
-
Alasaad S.
-
Otiende M.
Abstract
Pilobolus fungi are abundant in herbivore dung. Species identification within the genus has relied on morphological data, which lacks accuracy due to the overlap of several characters. In this study we applied molecular identification to assess the presence of Pilobolus species. This method is more reliable than the previously used morphometric analyses and the generated sequences can be used for future work. Dung samples were collected from different herbivores in Nairobi National Park and sporangium isolates were obtained for pure cultures. DNA was extracted from the pure cultures and Internal Transcribed Spacer regions amplified and sequenced. Two species of Pilobolus were identified, Pilobolus pullus, with a high genetic affinity, and a second cryptic species, morphologically identified as P. crystallinus, matched Pilobolus heterosporus. Lack of identification of other Pilobolus species, known as key species in lungworm transmission, may suggest the absence of such in the sampled area.
Keywords
Pilobolus, fungi, ITS, Phylogeny, Kenya
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S18210
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref25047,
author = {Antoinette Alouch and michael joseph jowers and Paul G mungai and Samer Alasaad and Moses Otiende},
title = {First molecular identification of Pilobolus fungi from Africa (Kenya)},
year = {2015},
keywords = {Pilobolus, fungi, ITS, Phylogeny, Kenya},
doi = {},
url = {http://},
pmid = {},
journal = {Mycoscience},
volume = {},
number = {},
pages = {},
abstract = {Pilobolus fungi are abundant in herbivore dung. Species identification within the genus has relied on morphological data, which lacks accuracy due to the overlap of several characters. In this study we applied molecular identification to assess the presence of Pilobolus species. This method is more reliable than the previously used morphometric analyses and the generated sequences can be used for future work. Dung samples were collected from different herbivores in Nairobi National Park and sporangium isolates were obtained for pure cultures. DNA was extracted from the pure cultures and Internal Transcribed Spacer regions amplified and sequenced. Two species of Pilobolus were identified, Pilobolus pullus, with a high genetic affinity, and a second cryptic species, morphologically identified as P. crystallinus, matched Pilobolus heterosporus. Lack of identification of other Pilobolus species, known as key species in lungworm transmission, may suggest the absence of such in the sampled area. }
}
- Show RIS reference
TY - JOUR
ID - 25047
AU - Alouch,Antoinette
AU - jowers,michael joseph
AU - mungai,Paul G
AU - Alasaad,Samer
AU - Otiende,Moses
T1 - First molecular identification of Pilobolus fungi from Africa (Kenya)
PY - 2015
KW - Pilobolus
KW - fungi
KW - ITS
KW - Phylogeny
KW - Kenya
UR - http://dx.doi.org/
N2 - Pilobolus fungi are abundant in herbivore dung. Species identification within the genus has relied on morphological data, which lacks accuracy due to the overlap of several characters. In this study we applied molecular identification to assess the presence of Pilobolus species. This method is more reliable than the previously used morphometric analyses and the generated sequences can be used for future work. Dung samples were collected from different herbivores in Nairobi National Park and sporangium isolates were obtained for pure cultures. DNA was extracted from the pure cultures and Internal Transcribed Spacer regions amplified and sequenced. Two species of Pilobolus were identified, Pilobolus pullus, with a high genetic affinity, and a second cryptic species, morphologically identified as P. crystallinus, matched Pilobolus heterosporus. Lack of identification of other Pilobolus species, known as key species in lungworm transmission, may suggest the absence of such in the sampled area.
L3 -
JF - Mycoscience
VL -
IS -
ER -
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