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Citation for Study 17154

Citation title: "Phylogenomics of asexual Epichloe fungal endophytes forming associations with perennial ryegrass (Lolium perenne L.)".

Study name: "Phylogenomics of asexual Epichloe fungal endophytes forming associations with perennial ryegrass (Lolium perenne L.)".

This study is part of submission 17154 (Status: Published).

Citation

Hettiarachchige I.K., Ekanayake P.N., Mann R.C., Guthridge K.M., Sawbridge T.I., Spangenberg G.C., & Forster J.W. 2015. Phylogenomics of asexual Epichloe fungal endophytes forming associations with perennial ryegrass (Lolium perenne L.). BMC Evolutionary Biology, .

Authors

• Hettiarachchige I.K. (submitter) +64 03 9032 7078
• Ekanayake P.N.
• Mann R.C.
• Guthridge K.M.
• Sawbridge T.I.
• Spangenberg G.C.
• Forster J.W.

Abstract

Background: Perennial ryegrass (Lolium perenne L.) is one of the most important species for temperate pastoral agriculture, forming associations with genetically diverse groups of mutualistic fungal endophytes. However, only two taxonomic groups (Epichloe festucae var. lolii and LpTG-2) have so far been described. In addition to these two well-characterised species, a third distinct group of previously unclassified perennial ryegrass-associated endophytes was identified as belonging to a putative novel taxon (or taxa) (PNT) in a previous analysis based on simple sequence repeat (SSR) marker diversity. In addition to genotypic differences, distinctive alkaloid production profiles were observed for members of the PNT class. Results: A detailed phylogenetic analysis of perennial ryegrass-associated endophytes using components of whole genome sequence data was performed using complete sequences of 7 nuclear protein-encoding genes. Three independently selected genes (encoding a DEAD/DEAH box helicase [Sbp4], a glycosyl hydrolase [family 92 protein] and a MEAB protein), none of which have been previously used for biosystematic studies of endophytes, were selected together with the frequently used ?house-keeping? genes tefA and tubB (encoding translation elongation factor 1-a and β-tubulin, respectively). In addition, two classes of endophyte-specific gene (perA for peramine biosynthesis and MT for mating-type control) were included. The results supported previous phylogenomic inferences for the known species, but distinctive patterns of diversity for the previously unclassified endophyte strains, which were further proposed to belong not one but two distinct novel taxa. Potential progenitor genomes for the asexual endophytes among contemporary anamorphic (Epichlo?) species were also identified from the phylogenetic analysis. Conclusions: Unique taxonomic status for the PNT was confirmed through comparison of multiple nuclear gene sequences, and also supported by evidence from chemotypic diversity. Analysis of MT gene idiomorphs further supported a predicted independent origin of two distinct perennial ryegrass-associated novel taxa, designated LpTG-3 and LpTG-4, from different members of a similar founder population related to contemporary E. festucae. The analysis also provided higher resolution to the known progenitor contributions of previously characterised perennial ryegrass-associated endophyte taxa.

Keywords

pasture grass, whole genome sequencing, taxonomy, nuclear gene, mating type, progenitor

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S17154
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref24231,
  	 author = {Inoka K Hettiarachchige and Piyumi  N Ekanayake and Ross C Mann and Kathryn M Guthridge and Timothy I Sawbridge and German C Spangenberg and John W Forster},
  	 title = {Phylogenomics of asexual Epichloe fungal endophytes forming associations with perennial ryegrass (Lolium perenne L.)},
  	 year = {2015},
  	 keywords = {pasture grass, whole genome sequencing, taxonomy, nuclear gene, mating type, progenitor},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BMC Evolutionary Biology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Background:
  Perennial ryegrass (Lolium perenne L.) is one of the most important species for temperate pastoral agriculture, forming associations with genetically diverse groups of mutualistic fungal endophytes. However, only two taxonomic groups (Epichloe festucae var. lolii and LpTG-2) have so far been described. In addition to these two well-characterised species, a third distinct group of previously unclassified perennial ryegrass-associated endophytes was identified as belonging to a putative novel taxon (or taxa) (PNT) in a previous analysis based on simple sequence repeat (SSR) marker diversity. In addition to genotypic differences, distinctive alkaloid production profiles were observed for members of the PNT class. 
  Results:
  A detailed phylogenetic analysis of perennial ryegrass-associated endophytes using components of whole genome sequence data was performed using complete sequences of 7 nuclear protein-encoding genes. Three independently selected genes (encoding a DEAD/DEAH box helicase [Sbp4], a glycosyl hydrolase [family 92 protein] and a MEAB protein), none of which have been previously used for  biosystematic studies of endophytes, were selected together with the frequently used ?house-keeping? genes tefA and tubB (encoding translation elongation factor 1-a and β-tubulin, respectively). In addition, two classes of endophyte-specific gene (perA for peramine biosynthesis and MT for mating-type control) were included. The results supported previous phylogenomic inferences for the known species, but distinctive patterns of diversity for the previously unclassified endophyte strains, which were further proposed to belong not one but two distinct novel taxa. Potential progenitor genomes for the asexual endophytes among contemporary anamorphic (Epichlo?) species were also identified from the phylogenetic analysis.
  Conclusions:
  Unique taxonomic status for the PNT was confirmed through comparison of multiple nuclear gene sequences, and also supported by evidence from chemotypic diversity. Analysis of MT gene idiomorphs further supported a predicted independent origin of two distinct perennial ryegrass-associated novel taxa, designated LpTG-3 and LpTG-4, from different members of a similar founder population related to contemporary E. festucae. The analysis also provided higher resolution to the known progenitor contributions of previously characterised perennial ryegrass-associated endophyte taxa. 
  }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 24231
  AU  - Hettiarachchige,Inoka K
  AU  - Ekanayake,Piyumi  N
  AU  - Mann,Ross C
  AU  - Guthridge,Kathryn M
  AU  - Sawbridge,Timothy I
  AU  - Spangenberg,German C
  AU  - Forster,John W
  T1  - Phylogenomics of asexual Epichloe fungal endophytes forming associations with perennial ryegrass (Lolium perenne L.)
  PY  - 2015
  KW  - pasture grass
  KW  - whole genome sequencing
  KW  - taxonomy
  KW  - nuclear gene
  KW  - mating type
  KW  - progenitor
  UR  - http://dx.doi.org/
  N2  - Background:
  Perennial ryegrass (Lolium perenne L.) is one of the most important species for temperate pastoral agriculture, forming associations with genetically diverse groups of mutualistic fungal endophytes. However, only two taxonomic groups (Epichloe festucae var. lolii and LpTG-2) have so far been described. In addition to these two well-characterised species, a third distinct group of previously unclassified perennial ryegrass-associated endophytes was identified as belonging to a putative novel taxon (or taxa) (PNT) in a previous analysis based on simple sequence repeat (SSR) marker diversity. In addition to genotypic differences, distinctive alkaloid production profiles were observed for members of the PNT class. 
  Results:
  A detailed phylogenetic analysis of perennial ryegrass-associated endophytes using components of whole genome sequence data was performed using complete sequences of 7 nuclear protein-encoding genes. Three independently selected genes (encoding a DEAD/DEAH box helicase [Sbp4], a glycosyl hydrolase [family 92 protein] and a MEAB protein), none of which have been previously used for  biosystematic studies of endophytes, were selected together with the frequently used ?house-keeping? genes tefA and tubB (encoding translation elongation factor 1-a and β-tubulin, respectively). In addition, two classes of endophyte-specific gene (perA for peramine biosynthesis and MT for mating-type control) were included. The results supported previous phylogenomic inferences for the known species, but distinctive patterns of diversity for the previously unclassified endophyte strains, which were further proposed to belong not one but two distinct novel taxa. Potential progenitor genomes for the asexual endophytes among contemporary anamorphic (Epichlo?) species were also identified from the phylogenetic analysis.
  Conclusions:
  Unique taxonomic status for the PNT was confirmed through comparison of multiple nuclear gene sequences, and also supported by evidence from chemotypic diversity. Analysis of MT gene idiomorphs further supported a predicted independent origin of two distinct perennial ryegrass-associated novel taxa, designated LpTG-3 and LpTG-4, from different members of a similar founder population related to contemporary E. festucae. The analysis also provided higher resolution to the known progenitor contributions of previously characterised perennial ryegrass-associated endophyte taxa. 
  
  L3  - 
  JF  - BMC Evolutionary Biology
  VL  - 
  IS  - 
  ER  -