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Citation for Study 26150

Citation title: "An endophyte of Macrochloa tenacissima (esparto or needle grass) from Tunisia is a novel species in the Fusarium redolens species complex ".

Study name: "An endophyte of Macrochloa tenacissima (esparto or needle grass) from Tunisia is a novel species in the Fusarium redolens species complex ".

This study is part of submission 26150 (Status: Published).

Citation

Gargouri S., Balmas V., Burgess L.W., Paulitz T., Laraba I., Kim H., Proctor R.H., Busman M., Felker F.C., Murray T., & Odonnell K. 2020. An endophyte of Macrochloa tenacissima (esparto or needle grass) from Tunisia is a novel species in the Fusarium redolens species complex. Mycologia, .

Authors

• Gargouri S.
• Balmas V.
• Burgess L.W.
• Paulitz T.
• Laraba I. (submitter) 3095669898
• Kim H.
• Proctor R.H.
• Busman M.
• Felker F.C.
• Murray T.
• Odonnell K.

Abstract

Here we report on the morphological, molecular and chemical characterization of a novel Fusarium species recovered from the roots and rhizosphere of Macrochloa tenacissima (Halfa, esparto or needle grass) in central Tunisia. Formally described here as F. spartum, this species is a member of the Fusarium redolens species complex, but differs from the other two species within the complex, F. redolens and F. hostae, by its endophytic association with M. tenacissima, and its genealogical exclusivity based on multilocus phylogenetic analyses. To assess their sexual reproductive mode, a PCR assay was designed and used to screen the three strains of F. spartum, 51 of F. redolens and 14 of F. hostae for mating type (MAT) idiomorph. Genetic architecture of the MAT locus in the former two species suggests that if they reproduce sexually it is via obligate outcrossing. By comparison, results of the PCR assay indicated that 13/14 of the F. hostae strains possessed MAT1-1 and MAT1-2 idiomorphs and thus might be self-fertile or homothallic. However, when the F. hostae strains were selfed, 11 failed to produce perithecia and one only produced several small abortive perithecia. Cirrhi with ascospores, however, were only produced by 8/28 and 4/84 of the variable size perithecia, respectively, of F. hostae NRRL 29888 and 29890. The potential for the three F. redolens clade species to produce mycotoxins, pigments and phytohormones was assessed by screening whole genome sequence data and by analyzing extracts on cracked maize kernel cultures via liquid chromatography-mass spectrometry.

Keywords

heterothallic, homothallic, MAT idiomorphs, molecular phylogenetics, morphology, RPB1, RPB2, TEF1, whole genome sequence, 1 new taxon

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S26150
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref30717,
  	 author = {Samia  Gargouri and Virgilio  Balmas and Lester W Burgess and Timothy   Paulitz and Imane  Laraba and Hye-Seon  Kim and Robert H. Proctor and Mark  Busman and Frederick C Felker and Timothy   Murray and Kerry  ODonnell},
  	 title = {An endophyte of Macrochloa tenacissima (esparto or needle grass) from Tunisia is a novel species in the Fusarium redolens species complex },
  	 year = {2020},
  	 keywords = {heterothallic, homothallic, MAT idiomorphs, molecular  phylogenetics, morphology, RPB1, RPB2, TEF1, whole genome sequence, 1 new taxon},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Mycologia},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Here we report on the morphological, molecular and chemical characterization of a novel Fusarium species recovered from the roots and rhizosphere of Macrochloa tenacissima (Halfa, esparto or needle grass) in central Tunisia.  Formally described here as F. spartum, this species is a member of the Fusarium redolens species complex, but differs from the other two species within the complex, F. redolens and F. hostae, by its endophytic association with M. tenacissima, and its genealogical exclusivity based on multilocus phylogenetic analyses.  To assess their sexual reproductive mode, a PCR assay was designed and used to screen the three strains of F. spartum, 51 of F. redolens and 14 of F. hostae for mating type (MAT) idiomorph.  Genetic architecture of the MAT locus in the former two species suggests that if they reproduce sexually it is via obligate outcrossing.  By comparison, results of the PCR assay indicated that 13/14 of the F. hostae strains possessed MAT1-1 and MAT1-2 idiomorphs and thus might be self-fertile or homothallic.  However, when the F. hostae strains were selfed, 11 failed to produce perithecia and one only produced several small abortive perithecia.  Cirrhi with ascospores, however, were only produced by 8/28 and 4/84 of the variable size perithecia, respectively, of F. hostae NRRL 29888 and 29890. The potential for the three F. redolens clade species to produce mycotoxins, pigments and phytohormones was assessed by screening whole genome sequence data and by analyzing extracts on cracked maize kernel cultures via liquid chromatography-mass spectrometry.  }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 30717
  AU  - Gargouri,Samia 
  AU  - Balmas,Virgilio 
  AU  - Burgess,Lester W
  AU  - Paulitz,Timothy  
  AU  - Laraba,Imane 
  AU  - Kim,Hye-Seon 
  AU  - Proctor,Robert H.
  AU  - Busman,Mark 
  AU  - Felker,Frederick C
  AU  - Murray,Timothy  
  AU  - ODonnell,Kerry 
  T1  - An endophyte of Macrochloa tenacissima (esparto or needle grass) from Tunisia is a novel species in the Fusarium redolens species complex 
  PY  - 2020
  KW  - heterothallic
  KW  - homothallic
  KW  - MAT idiomorphs
  KW  - molecular  phylogenetics
  KW  - morphology
  KW  - RPB1
  KW  - RPB2
  KW  - TEF1
  KW  - whole genome sequence
  KW  - 1 new taxon
  UR  - http://dx.doi.org/
  N2  - Here we report on the morphological, molecular and chemical characterization of a novel Fusarium species recovered from the roots and rhizosphere of Macrochloa tenacissima (Halfa, esparto or needle grass) in central Tunisia.  Formally described here as F. spartum, this species is a member of the Fusarium redolens species complex, but differs from the other two species within the complex, F. redolens and F. hostae, by its endophytic association with M. tenacissima, and its genealogical exclusivity based on multilocus phylogenetic analyses.  To assess their sexual reproductive mode, a PCR assay was designed and used to screen the three strains of F. spartum, 51 of F. redolens and 14 of F. hostae for mating type (MAT) idiomorph.  Genetic architecture of the MAT locus in the former two species suggests that if they reproduce sexually it is via obligate outcrossing.  By comparison, results of the PCR assay indicated that 13/14 of the F. hostae strains possessed MAT1-1 and MAT1-2 idiomorphs and thus might be self-fertile or homothallic.  However, when the F. hostae strains were selfed, 11 failed to produce perithecia and one only produced several small abortive perithecia.  Cirrhi with ascospores, however, were only produced by 8/28 and 4/84 of the variable size perithecia, respectively, of F. hostae NRRL 29888 and 29890. The potential for the three F. redolens clade species to produce mycotoxins, pigments and phytohormones was assessed by screening whole genome sequence data and by analyzing extracts on cracked maize kernel cultures via liquid chromatography-mass spectrometry.  
  L3  - 
  JF  - Mycologia
  VL  - 
  IS  - 
  ER  -