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Citation for Study 372

Citation title: "Phylogeny of Cryphonectria cubensis and allied species inferred from DNA analysis.".

This study was previously identified under the legacy study ID S306 (Status: Published).

Citation

Myburg H., Wingfield B.D., & Wingfield M.J. 1999. Phylogeny of Cryphonectria cubensis and allied species inferred from DNA analysis. Mycologia, 91(2): 243-250.

Authors

Myburg H.
Wingfield B.D.
Wingfield M.J.

Abstract

Cryphonectria cubensis causes a serious canker disease of Eucalyptus in many tropical areas of the world. The aim of this study was to determine the phylogenetic relationships among strains of C. cubensis and the related species C. parasitica, Endothia eugeniae and E. gyrosa using DNA sequence data. A second objective was to develop a rapid diagnostic method to distinguish among these taxa. The variable ITS1 and ITS2 regions, as well as the conserved 5.8S gene of the rRNA operon, were amplified and sequenced. The amplified PCR products were also used in Alu I and Cfo I restriction digests. Analysis of the restriction profiles indicate that it is possible to distinguish between C. cubensis, C. parasitica, E. eugeniae and E. gyrosa using RFLPs (restriction fragment length polymorphisms). This will facilitate the identification of isolates thought to represent C. cubensis that sporulate poorly in culture. Analysis of the sequence data showed that C. cubensis isolates form a distinct clade that includes a strain from clove that might otherwise have been assigned the name E. eugeniae. Isolates of C. parasitica formed a separate clade. Endothia gyrosa grouped separately from both C. cubensis and C. parasitica. Key Words: Cryphonectria parasitica, Endothia eugeniae, Endothia gyrosa, Eucalyptus, RFLPs, Ribosomal RNA

Keywords

Cryphonectria parasitica; Endothia eugeniae; Endothia gyrosa; Eucalyptus; RFLPs; ribosomal RNA

External links

Other URL: http://www.jstor.org/stable/3761369

About this resource

Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S372
Other versions: Nexus NeXML

Show BibTeX reference

		@ARTICLE{TreeBASE2Ref16804,
	 author = {Henrietta  Myburg and Brenda D Wingfield and Michael J Wingfield},
	 title = {Phylogeny of Cryphonectria cubensis and allied species inferred from DNA analysis.},
	 year = {1999},
	 keywords = {Cryphonectria parasitica; Endothia eugeniae; Endothia gyrosa; Eucalyptus; RFLPs; ribosomal RNA},
	 doi = {},
	 url = {http://www.jstor.org/stable/3761369},
	 pmid = {},
	 journal = {Mycologia},
	 volume = {91},
	 number = {2},
	 pages = {243--250},
	 abstract = {Cryphonectria cubensis causes a serious canker disease of Eucalyptus in many tropical areas of the world. The aim of this study was to determine the phylogenetic relationships among strains of C. cubensis and the related species C. parasitica, Endothia eugeniae and E. gyrosa using DNA sequence data. A second objective was to develop a rapid diagnostic method to distinguish among these taxa. The variable ITS1 and ITS2 regions, as well as the conserved 5.8S gene of the rRNA operon, were amplified and sequenced. The amplified PCR products were also used in Alu I and Cfo I restriction digests. Analysis of the restriction profiles indicate that it is possible to distinguish between C. cubensis, C. parasitica, E. eugeniae and E. gyrosa using RFLPs (restriction fragment length polymorphisms). This will facilitate the identification of isolates thought to represent C. cubensis that sporulate poorly in culture. Analysis of the sequence data showed that C. cubensis isolates form a distinct clade that includes a strain from clove that might otherwise have been assigned the name E. eugeniae. Isolates of C. parasitica formed a separate clade. Endothia gyrosa grouped separately from both C. cubensis and C. parasitica. Key Words: Cryphonectria parasitica, Endothia eugeniae, Endothia gyrosa, Eucalyptus, RFLPs, Ribosomal RNA}
}

Show RIS reference

TY - JOUR
ID - 16804
AU - Myburg,Henrietta
AU - Wingfield,Brenda D
AU - Wingfield,Michael J
T1 - Phylogeny of Cryphonectria cubensis and allied species inferred from DNA analysis.
PY - 1999
KW - Cryphonectria parasitica; Endothia eugeniae; Endothia gyrosa; Eucalyptus; RFLPs; ribosomal RNA
UR - http://www.jstor.org/stable/3761369
N2 - Cryphonectria cubensis causes a serious canker disease of Eucalyptus in many tropical areas of the world. The aim of this study was to determine the phylogenetic relationships among strains of C. cubensis and the related species C. parasitica, Endothia eugeniae and E. gyrosa using DNA sequence data. A second objective was to develop a rapid diagnostic method to distinguish among these taxa. The variable ITS1 and ITS2 regions, as well as the conserved 5.8S gene of the rRNA operon, were amplified and sequenced. The amplified PCR products were also used in Alu I and Cfo I restriction digests. Analysis of the restriction profiles indicate that it is possible to distinguish between C. cubensis, C. parasitica, E. eugeniae and E. gyrosa using RFLPs (restriction fragment length polymorphisms). This will facilitate the identification of isolates thought to represent C. cubensis that sporulate poorly in culture. Analysis of the sequence data showed that C. cubensis isolates form a distinct clade that includes a strain from clove that might otherwise have been assigned the name E. eugeniae. Isolates of C. parasitica formed a separate clade. Endothia gyrosa grouped separately from both C. cubensis and C. parasitica. Key Words: Cryphonectria parasitica, Endothia eugeniae, Endothia gyrosa, Eucalyptus, RFLPs, Ribosomal RNA
L3 -
JF - Mycologia
VL - 91
IS - 2
SP - 243
EP - 250
ER -