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Citation for Study 16007

About Citation title: "Phylogeny and recombination analysis of Lily symptomless virus and Lily mottle virus from Brazil ".
About Study name: "Phylogeny and recombination analysis of Lily symptomless virus and Lily mottle virus from Brazil ".
About This study is part of submission 16007 (Status: Published).

Citation

Rivas E.B., B?di E.C., Harakava R., Gregori F., & Goncalves M.C. 2014. Phylogeny and recombination analysis of Lily symptomless virus and Lily mottle virus from Brazil. Plant Disease, .

Authors

  • Rivas E.B. Phone 55-11-50871722
  • B?di E.C.
  • Harakava R. Phone 55-11-55490114
  • Gregori F. (submitter) Phone 55 11 30911386
  • Goncalves M.C.

Abstract

Lilies are cultivated in Brazil from imported bulbs that might be infected by exotic or quarantine viruses, such as Lily mottle virus (LMoV) and Lily symptomless virus (LSV). Since LMoV and LSV have been recently reported in Brazil, we detected the spreading of these viruses in 12 cultivation areas using simplex and multiplex RT-PCR with specific primers designed for the respective coat protein (CP) genes amplification. Eight fragments with 800 nt length obtained from the LMoV-infected and nine with 600 nt from LSV- infected lilies were sequenced. Phylogenetic analysis using the CP sequences clustered two Brazilian LMoV isolates in the same subtree with Tulip breaking virus-lily strain (sin. LMoV) and Tulip band breaking virus, both from Japan. The other six LMoV Brazilian isolates share a common ancestor with isolates from different countries. LSV isolates from Brazil clustered with isolates from Asia and The Netherlands, sharing a common ancestor.Recombination detection analysis with partial CP sequences from both viruses revealed a LMoV recombinant isolate (S44147-Netherlands) whereas none event was supported for recombination among LSV isolates. To our knowledge, this is the ?rst report on evidence of recombination between LMoV isolates and of a proposal for its classification into groups.

Keywords

Carlavirus, Potyvirus, Multiplex PCR

External links

About this resource

  • Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S16007
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