@ARTICLE{TreeBASE2Ref28700,
author = {Marco Antonio Bautista-Cruz and Gustavo Almaguer-Vargas and Santos Gerardo Leyva-Mir and Maria Teresa Colinas-Le?n and Kamila Camara Correia and Mois?s Camacho-Tapia and Leticia Robles-Yerena and Sami Jorge Michereff and Juan Manoel Tovar-Pedraza},
title = {Phylogeny, Distribution, and Pathogenicity of Lasiodiplodia Species Associated With Cankers and Dieback Symptoms of Persian Lime in Mexico.},
year = {2019},
keywords = {Citrus latifolia, dieback, pathogenicity, virulence, phylogenetic inference},
doi = {10.1094/PDIS-06-18-1036-RE},
url = {http://apsjournals.apsnet.org/doi/10.1094/PDIS-06-18-1036-RE},
pmid = {},
journal = {Plant Disease},
volume = {},
number = {},
pages = {},
abstract = {Persian lime (Citrus latifolia Tan.) is an important and widely cultivated fruit crop in several regions of Mexico. In recent years, severe symptoms of gummosis, stem cankers, and dieback were detected in the Persian lime-producing region in the states of Veracruz and Puebla, Mexico. The aims of this study were to identify the species of Lasiodiplodia associated with these symptoms, determine the distribution of these species and test their pathogenicity and virulence on Persian lime plants. In 2015, symptomatic samples of 12 commercial Persian lime orchards were collected and 60 Lasiodiplodia isolates were obtained. Fungal identification of 32 representative isolates was performed using a phylogenetic analysis based on DNA sequence data of the internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α (EF1-α), and β-tubulin (BT2) genes. Sequence analysis was carried out using the Maximum Likelihood and Bayesian Inference methods. Six Lasiodiplodia species were identified as L. pseudotheobromae, L. theobromae, L. brasiliense, L. subglobosa, L. citricola and L. iraniensis. All Lasiodiplodia species of this study are reported for first time in association with Persian lime in Mexico and worldwide. Lasiodiplodia pseudotheobromae (42% of isolates) was the most frequently isolated species, followed by L. theobromae (28%) and L. brasiliense (12.5%). Pathogenicity on Persian lime young plant using a mycelial plug inoculation method showed that all identified Lasiodiplodia species were able to cause necrotic lesions and gummosis, but L. subglobosa, L. iraniensis, and L. pseudotheobromae were the most virulent.}
}
Citation for Study 23111
Citation title:
"Phylogeny, Distribution, and Pathogenicity of Lasiodiplodia Species Associated With Cankers and Dieback Symptoms of Persian Lime in Mexico.".
Study name:
"Phylogeny, Distribution, and Pathogenicity of Lasiodiplodia Species Associated With Cankers and Dieback Symptoms of Persian Lime in Mexico.".
This study is part of submission 23111
(Status: Published).
Citation
Bautista-cruz M.A., Almaguer-vargas G., Leyva-mir S.G., Colinas-le?n M.T., Correia K.C., Camacho-tapia M., Robles-yerena L., Michereff S.J., & Tovar-pedraza J.M. 2019. Phylogeny, Distribution, and Pathogenicity of Lasiodiplodia Species Associated With Cankers and Dieback Symptoms of Persian Lime in Mexico. Plant Disease, .
Authors
-
Bautista-cruz M.A.
-
Almaguer-vargas G.
-
Leyva-mir S.G.
-
Colinas-le?n M.T.
-
Correia K.C.
(submitter)
005588996079448
-
Camacho-tapia M.
-
Robles-yerena L.
-
Michereff S.J.
-
Tovar-pedraza J.M.
Abstract
Persian lime (Citrus latifolia Tan.) is an important and widely cultivated fruit crop in several regions of Mexico. In recent years, severe symptoms of gummosis, stem cankers, and dieback were detected in the Persian lime-producing region in the states of Veracruz and Puebla, Mexico. The aims of this study were to identify the species of Lasiodiplodia associated with these symptoms, determine the distribution of these species and test their pathogenicity and virulence on Persian lime plants. In 2015, symptomatic samples of 12 commercial Persian lime orchards were collected and 60 Lasiodiplodia isolates were obtained. Fungal identification of 32 representative isolates was performed using a phylogenetic analysis based on DNA sequence data of the internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α (EF1-α), and β-tubulin (BT2) genes. Sequence analysis was carried out using the Maximum Likelihood and Bayesian Inference methods. Six Lasiodiplodia species were identified as L. pseudotheobromae, L. theobromae, L. brasiliense, L. subglobosa, L. citricola and L. iraniensis. All Lasiodiplodia species of this study are reported for first time in association with Persian lime in Mexico and worldwide. Lasiodiplodia pseudotheobromae (42% of isolates) was the most frequently isolated species, followed by L. theobromae (28%) and L. brasiliense (12.5%). Pathogenicity on Persian lime young plant using a mycelial plug inoculation method showed that all identified Lasiodiplodia species were able to cause necrotic lesions and gummosis, but L. subglobosa, L. iraniensis, and L. pseudotheobromae were the most virulent.
Keywords
Citrus latifolia, dieback, pathogenicity, virulence, phylogenetic inference
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S23111
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref28700,
author = {Marco Antonio Bautista-Cruz and Gustavo Almaguer-Vargas and Santos Gerardo Leyva-Mir and Maria Teresa Colinas-Le?n and Kamila Camara Correia and Mois?s Camacho-Tapia and Leticia Robles-Yerena and Sami Jorge Michereff and Juan Manoel Tovar-Pedraza},
title = {Phylogeny, Distribution, and Pathogenicity of Lasiodiplodia Species Associated With Cankers and Dieback Symptoms of Persian Lime in Mexico.},
year = {2019},
keywords = {Citrus latifolia, dieback, pathogenicity, virulence, phylogenetic inference},
doi = {10.1094/PDIS-06-18-1036-RE},
url = {http://apsjournals.apsnet.org/doi/10.1094/PDIS-06-18-1036-RE},
pmid = {},
journal = {Plant Disease},
volume = {},
number = {},
pages = {},
abstract = {Persian lime (Citrus latifolia Tan.) is an important and widely cultivated fruit crop in several regions of Mexico. In recent years, severe symptoms of gummosis, stem cankers, and dieback were detected in the Persian lime-producing region in the states of Veracruz and Puebla, Mexico. The aims of this study were to identify the species of Lasiodiplodia associated with these symptoms, determine the distribution of these species and test their pathogenicity and virulence on Persian lime plants. In 2015, symptomatic samples of 12 commercial Persian lime orchards were collected and 60 Lasiodiplodia isolates were obtained. Fungal identification of 32 representative isolates was performed using a phylogenetic analysis based on DNA sequence data of the internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α (EF1-α), and β-tubulin (BT2) genes. Sequence analysis was carried out using the Maximum Likelihood and Bayesian Inference methods. Six Lasiodiplodia species were identified as L. pseudotheobromae, L. theobromae, L. brasiliense, L. subglobosa, L. citricola and L. iraniensis. All Lasiodiplodia species of this study are reported for first time in association with Persian lime in Mexico and worldwide. Lasiodiplodia pseudotheobromae (42% of isolates) was the most frequently isolated species, followed by L. theobromae (28%) and L. brasiliense (12.5%). Pathogenicity on Persian lime young plant using a mycelial plug inoculation method showed that all identified Lasiodiplodia species were able to cause necrotic lesions and gummosis, but L. subglobosa, L. iraniensis, and L. pseudotheobromae were the most virulent.}
}
- Show RIS reference
TY - JOUR
ID - 28700
AU - Bautista-Cruz,Marco Antonio
AU - Almaguer-Vargas,Gustavo
AU - Leyva-Mir,Santos Gerardo
AU - Colinas-Le?n,Maria Teresa
AU - Correia,Kamila Camara
AU - Camacho-Tapia,Mois?s
AU - Robles-Yerena,Leticia
AU - Michereff,Sami Jorge
AU - Tovar-Pedraza,Juan Manoel
T1 - Phylogeny, Distribution, and Pathogenicity of Lasiodiplodia Species Associated With Cankers and Dieback Symptoms of Persian Lime in Mexico.
PY - 2019
KW - Citrus latifolia
KW - dieback
KW - pathogenicity
KW - virulence
KW - phylogenetic inference
UR - http://apsjournals.apsnet.org/doi/10.1094/PDIS-06-18-1036-RE
N2 - Persian lime (Citrus latifolia Tan.) is an important and widely cultivated fruit crop in several regions of Mexico. In recent years, severe symptoms of gummosis, stem cankers, and dieback were detected in the Persian lime-producing region in the states of Veracruz and Puebla, Mexico. The aims of this study were to identify the species of Lasiodiplodia associated with these symptoms, determine the distribution of these species and test their pathogenicity and virulence on Persian lime plants. In 2015, symptomatic samples of 12 commercial Persian lime orchards were collected and 60 Lasiodiplodia isolates were obtained. Fungal identification of 32 representative isolates was performed using a phylogenetic analysis based on DNA sequence data of the internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α (EF1-α), and β-tubulin (BT2) genes. Sequence analysis was carried out using the Maximum Likelihood and Bayesian Inference methods. Six Lasiodiplodia species were identified as L. pseudotheobromae, L. theobromae, L. brasiliense, L. subglobosa, L. citricola and L. iraniensis. All Lasiodiplodia species of this study are reported for first time in association with Persian lime in Mexico and worldwide. Lasiodiplodia pseudotheobromae (42% of isolates) was the most frequently isolated species, followed by L. theobromae (28%) and L. brasiliense (12.5%). Pathogenicity on Persian lime young plant using a mycelial plug inoculation method showed that all identified Lasiodiplodia species were able to cause necrotic lesions and gummosis, but L. subglobosa, L. iraniensis, and L. pseudotheobromae were the most virulent.
L3 - 10.1094/PDIS-06-18-1036-RE
JF - Plant Disease
VL -
IS -
ER -