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Citation for Study 12343

Citation title: "Molecular phylogenetic analyses based on the nuclear rRNA genes and the intron-exon structures of the nuSSU rRNA gene in Dictyocatenulata alba (anamorphic Ascomycota)".

Study name: "Molecular phylogenetic analyses based on the nuclear rRNA genes and the intron-exon structures of the nuSSU rRNA gene in Dictyocatenulata alba (anamorphic Ascomycota)".

This study is part of submission 12343 (Status: Published).

Citation

An K., Degawa Y., Fujihara E., Mikawa T., Ohkuma M., & Okada G. 2012. Molecular phylogenetic analyses based on the nuclear rRNA genes and the intron-exon structures of the nuSSU rRNA gene in Dictyocatenulata alba (anamorphic Ascomycota). Fungal Biology, .

Authors

• An K.
• Degawa Y.
• Fujihara E.
• Mikawa T.
• Ohkuma M.
• Okada G.

Abstract

Molecular phylogenies inferred from the nuclear small subunit rRNA gene (nuSSU), nuclear large subunit rRNA gene D1/D2 region (nuLSU), and ITS-5.8S rRNA gene (ITS) indicated that five cultures of the lichenized hyphomycete Dictyocatenulata alba isolated from Japan form a monophyletic clade with high bootstrap support, and a close relationship to the Ostropomycetidae (Lecanoromycetes, Pezizomycotina, Ascomycota). Insertion sequences were found in the nuSSU of all isolates (e.g., nine insertions in the strain JCM 5358), some of which were group I introns. Five new insertion positions were found among the D. alba isolates. Using BLAST, none of the insertion sequences of D. alba were closely related to those of fungi or other organisms deposited in public DNA databases. Insertion positions were similar in some isolates, and two positions were common to all isolates. Although all phylogenetic analyses based on nuSSU, nuLSU, and ITS revealed the monophyly of D. alba, the isolates were divided into two (in the nuSSU tree) or three (in the nuLSU and ITS trees) groups. Based on the phylogenetic analyses and the intron-exon structures, the five isolates identified as D. alba belong to three cryptic species and therefore D. alba should be considered a species complex. The very slow-growing, tough agar colonies of the isolates, the occurrence of the species on both slightly lichenized and non-lichenized surfaces of trees, or pebbles (rarely on soil), suggest that the members of the D. alba complex may be lichenized. The photobiont was not clearly identified in this study.

Keywords

Cheiromycina ananas, Lichenized (lichenised) fungi, Ostropomycetidae, RT-PCR, Species complex

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S12343
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref20405,
  	 author = {Kwang Deuk  An and Yousuke  Degawa and Eriko  Fujihara and Takashi  Mikawa and Moriya  Ohkuma and Gen  Okada},
  	 title = {Molecular phylogenetic analyses based on the nuclear rRNA genes and the intron-exon structures of the nuSSU rRNA gene in Dictyocatenulata alba (anamorphic Ascomycota)},
  	 year = {2012},
  	 keywords = {Cheiromycina ananas, Lichenized (lichenised) fungi, Ostropomycetidae, RT-PCR, Species complex},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Fungal Biology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Molecular phylogenies inferred from the nuclear small subunit rRNA gene (nuSSU), nuclear
  large subunit rRNA gene D1/D2 region (nuLSU), and ITS-5.8S rRNA gene (ITS) indicated
  that five cultures of the lichenized hyphomycete Dictyocatenulata alba isolated from Japan
  form a monophyletic clade with high bootstrap support, and a close relationship to the
  Ostropomycetidae (Lecanoromycetes, Pezizomycotina, Ascomycota). Insertion sequences
  were found in the nuSSU of all isolates (e.g., nine insertions in the strain JCM 5358), some of
  which were group I introns. Five new insertion positions were found among the D. alba
  isolates. Using BLAST, none of the insertion sequences of D. alba were closely related to
  those of fungi or other organisms deposited in public DNA databases. Insertion positions were
  similar in some isolates, and two positions were common to all isolates. Although all
  phylogenetic analyses based on nuSSU, nuLSU, and ITS revealed the monophyly of D. alba,
  the isolates were divided into two (in the nuSSU tree) or three (in the nuLSU and ITS trees)
  groups. Based on the phylogenetic analyses and the intron-exon structures, the five isolates
  identified as D. alba belong to three cryptic species and therefore D. alba should be
  considered a species complex. The very slow-growing, tough agar colonies of the isolates, the
  occurrence of the species on both slightly lichenized and non-lichenized surfaces of trees, or
  pebbles (rarely on soil), suggest that the members of the D. alba complex may be lichenized.
  The photobiont was not clearly identified in this study.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 20405
  AU  - An,Kwang Deuk 
  AU  - Degawa,Yousuke 
  AU  - Fujihara,Eriko 
  AU  - Mikawa,Takashi 
  AU  - Ohkuma,Moriya 
  AU  - Okada,Gen 
  T1  - Molecular phylogenetic analyses based on the nuclear rRNA genes and the intron-exon structures of the nuSSU rRNA gene in Dictyocatenulata alba (anamorphic Ascomycota)
  PY  - 2012
  KW  - Cheiromycina ananas
  KW  - Lichenized (lichenised) fungi
  KW  - Ostropomycetidae
  KW  - RT-PCR
  KW  - Species complex
  UR  - http://dx.doi.org/
  N2  - Molecular phylogenies inferred from the nuclear small subunit rRNA gene (nuSSU), nuclear
  large subunit rRNA gene D1/D2 region (nuLSU), and ITS-5.8S rRNA gene (ITS) indicated
  that five cultures of the lichenized hyphomycete Dictyocatenulata alba isolated from Japan
  form a monophyletic clade with high bootstrap support, and a close relationship to the
  Ostropomycetidae (Lecanoromycetes, Pezizomycotina, Ascomycota). Insertion sequences
  were found in the nuSSU of all isolates (e.g., nine insertions in the strain JCM 5358), some of
  which were group I introns. Five new insertion positions were found among the D. alba
  isolates. Using BLAST, none of the insertion sequences of D. alba were closely related to
  those of fungi or other organisms deposited in public DNA databases. Insertion positions were
  similar in some isolates, and two positions were common to all isolates. Although all
  phylogenetic analyses based on nuSSU, nuLSU, and ITS revealed the monophyly of D. alba,
  the isolates were divided into two (in the nuSSU tree) or three (in the nuLSU and ITS trees)
  groups. Based on the phylogenetic analyses and the intron-exon structures, the five isolates
  identified as D. alba belong to three cryptic species and therefore D. alba should be
  considered a species complex. The very slow-growing, tough agar colonies of the isolates, the
  occurrence of the species on both slightly lichenized and non-lichenized surfaces of trees, or
  pebbles (rarely on soil), suggest that the members of the D. alba complex may be lichenized.
  The photobiont was not clearly identified in this study.
  L3  - 
  JF  - Fungal Biology
  VL  - 
  IS  - 
  ER  -