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Citation for Study 16630

About Citation title: "DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring".
About Study name: "DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring".
About This study is part of submission 16630 (Status: Published).

Citation

Frantine-silva W., Sofia S.H., Orsi M.L., & Almeida F.S. 2015. DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring. Molecular Ecology Resources, .

Authors

  • Frantine-silva W. (submitter) Phone 554399290927
  • Sofia S.H.
  • Orsi M.L.
  • Almeida F.S.

Abstract

Quantifying and classifying ichthyoplankton is one of the most effective ways of monitoring the recruitment process in fish. However, correctly identifying the fish based on morphological characters is extremely difficult, especially in the early stages of development. We examined ichthyoplankton from tributaries and reservoirs along the middle stretch of the Paranapanema River, one of the areas most impacted by hydroelectric projects in the Neotropics. Matching DNA sequences of the COI gene (628-648 bp) allowed us to identify 99.25% of 536 samples of eggs (293) and larvae (243) subjected to BOLD-IDS similarity analysis with a species-level threshold of 1.3%. The results revealed 37 species in 27 genera, 15 families and four orders, some 23.8% of documented fish species in the Paranapanema River. Molecular identification meant that we could include data from egg samples that accounted for about 30% of the species richness observed. The results in this study confirm the efficacy of DNA barcoding in identifying Neotropical ichthyoplankton and show how the data produced provide valuable information for preparing plans for conserving and managing inland waters.

Keywords

Ichthyoplankton, fish recruitment, conservation, upper Paraná River

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About this resource

  • Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S16630
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