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Citation for Study 10295

Citation title: "Sequence-based identification of Japanese Armillaria species using the elongation factor-1 alpha gene.".

This study was previously identified under the legacy study ID S2657 (Status: Published).

Citation

Hasegawa E., Ota Y., Hattori T., & Kikuchi T. 2010. Sequence-based identification of Japanese Armillaria species using the elongation factor-1 alpha gene. Mycologia, .

Authors

Hasegawa E.
Ota Y.
Hattori T.
Kikuchi T.

Abstract

We analyzed the sequences of 3 DNA regions?the translation elongation factor-1 alpha (EF-1 alpha) gene and the internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA?to compare their accuracy in identifying different species of Japanese Armillaria. We studied 49 isolates of 8 Armillaria species: A. mellea, A. ostoyae, A. nabsnona, A. cepistipes, A. gallica, A. sinapina, A. tabescens and the biological species Nagasawa E (Nag. E). The phylogenetic analyses of the ITS and IGS data helped in identifying A. mellea, A. ostoyae, A. nabsnona, A. tabescens and Nag. E, but could not be used to identify A. gallica, A. cepistipes and A. sinapina. Nevertheless, our analysis showed that the EF-1 alpha gene was clearly different in the 8 examined species. Restriction fragment length polymorphisms (RFLPs) of the IGS-1 region could be used to distinguish most species, but the RFLP profiles of some isolates of A. cepistipes and A. sinapina were the same even with 4 different restriction enzymes. In conclusion, among the techniques examined in this study, analyzing the EF-1 alpha sequence was found to be the most suitable method for identifying the different species of Japanese Armillaria.

Keywords

Armillaria, translation elongation factor-1 alpha gene, IGS, ITS, RFLP, species identification

External links

DOI: 10.3852/09-238

About this resource

Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S10295
Other versions: Nexus NeXML

Show BibTeX reference

		@ARTICLE{TreeBASE2Ref18785,
	 author = {Eri  Hasegawa and Yuko   Ota and Tsutomu  Hattori and Taisei  Kikuchi},
	 title = {Sequence-based identification of Japanese Armillaria species using the elongation factor-1 alpha gene.},
	 year = {2010},
	 keywords = {Armillaria, translation elongation factor-1 alpha gene, IGS, ITS, RFLP, species identification},
	 doi = {10.3852/09-238},
	 url = {},
	 pmid = {},
	 journal = {Mycologia},
	 volume = {},
	 number = {},
	 pages = {},
	 abstract = {We analyzed the sequences of 3 DNA regions?the translation elongation factor-1 alpha (EF-1 alpha) gene and the internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA?to compare their accuracy in identifying different species of Japanese Armillaria. We studied 49 isolates of 8 Armillaria species: A. mellea, A. ostoyae, A. nabsnona, A. cepistipes, A. gallica, A. sinapina, A. tabescens and the biological species Nagasawa E (Nag. E). The phylogenetic analyses of the ITS and IGS data helped in identifying A. mellea, A. ostoyae, A. nabsnona, A. tabescens and Nag. E, but could not be used to identify A. gallica, A. cepistipes and A. sinapina. Nevertheless, our analysis showed that the EF-1 alpha gene was clearly different in the 8 examined species. Restriction fragment length polymorphisms (RFLPs) of the IGS-1 region could be used to distinguish most species, but the RFLP profiles of some isolates of A. cepistipes and A. sinapina were the same even with 4 different restriction enzymes. In conclusion, among the techniques examined in this study, analyzing the EF-1 alpha sequence was found to be the most suitable method for identifying the different species of Japanese Armillaria.}
}

Show RIS reference

		TY  - JOUR
ID  - 18785
AU  - Hasegawa,Eri 
AU  - Ota,Yuko  
AU  - Hattori,Tsutomu 
AU  - Kikuchi,Taisei 
T1  - Sequence-based identification of Japanese Armillaria species using the elongation factor-1 alpha gene.
PY  - 2010
KW  - Armillaria
KW  - translation elongation factor-1 alpha gene
KW  - IGS
KW  - ITS
KW  - RFLP
KW  - species identification
UR  - 
N2  - We analyzed the sequences of 3 DNA regions?the translation elongation factor-1 alpha (EF-1 alpha) gene and the internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA?to compare their accuracy in identifying different species of Japanese Armillaria. We studied 49 isolates of 8 Armillaria species: A. mellea, A. ostoyae, A. nabsnona, A. cepistipes, A. gallica, A. sinapina, A. tabescens and the biological species Nagasawa E (Nag. E). The phylogenetic analyses of the ITS and IGS data helped in identifying A. mellea, A. ostoyae, A. nabsnona, A. tabescens and Nag. E, but could not be used to identify A. gallica, A. cepistipes and A. sinapina. Nevertheless, our analysis showed that the EF-1 alpha gene was clearly different in the 8 examined species. Restriction fragment length polymorphisms (RFLPs) of the IGS-1 region could be used to distinguish most species, but the RFLP profiles of some isolates of A. cepistipes and A. sinapina were the same even with 4 different restriction enzymes. In conclusion, among the techniques examined in this study, analyzing the EF-1 alpha sequence was found to be the most suitable method for identifying the different species of Japanese Armillaria.
L3  - 10.3852/09-238
JF  - Mycologia
VL  - 
IS  - 
ER  -