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Citation for Study 10386

Citation title: "Population genetic structure of the seed pathogen Pyrenophora semeniperda on Bromus tectorum in western North America".

Study name: "Population genetic structure of the seed pathogen Pyrenophora semeniperda on Bromus tectorum in western North America".

This study is part of submission 10376 (Status: Published).

Citation

Boose D.L., Harrison S., Clement S., & Meyer S. 2010. Population genetic structure of the seed pathogen Pyrenophora semeniperda on Bromus tectorum in western North America. Mycologia, .

Authors

• Boose D.L. (submitter) 509-313-6634
• Harrison S.
• Clement S.
• Meyer S.

Abstract

We examined genetic variation in the ascomycete pathogen Pyrenophora semeniperda cultured from seeds of the invasive grass Bromus tectorum in the Intermountain West of North America. We sequenced the internal transcribed spacer (ITS) region of the nuclear ribosomal RNA genome in 417 monoconidial cultures collected from 20 sites in Washington, Idaho, Utah, and Colorado, USA. ITS sequence diversity was surprisingly high; 12 unique haplotypes were identified, averaging 1.3% pairwise sequence divergence. All sites had at least two haplotypes present, and three sites had seven or more. One haplotype comprised 60% of the isolates and occurred at all 20 locations; the remaining haplotypes generally occurred at low frequencies within sites, but at multiple sites throughout the region. Sites in Washington and Idaho were more diverse than those in Utah and Colorado, averaging 2 more haplotypes and 67% more pairwise differences among haplotypes at a site. Analysis of Molecular Variance (AMOVA) indicated that more than 80% of the genetic variation is found within sampling locations, while 7 ? 11% of the variation can be attributed to differences between northern (Washington and Idaho) and southern (Utah and Colorado) populations. The wide distribution of even uncommon haplotypes among sampling sites, and weak correlations between genetic and geographic distances among populations (< 0.2), suggest that these populations were recently established from a common source. We hypothesize that the strains of P. semeniperda infecting B. tectorum in western North America probably arrived with the invasive grass from its native Eurasian range.

Keywords

Analysis of Molecular Variance (AMOVA), ascomycete, Drechslera campanulata, genetic diversity, internal transcribed spacer (ITS), invasive species, ribosomal RNA

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S10386
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref18858,
  	 author = {David L. Boose and Steven  Harrison and Suzette  Clement and Susan  Meyer},
  	 title = {Population genetic structure of the seed pathogen Pyrenophora semeniperda on Bromus tectorum in western North America},
  	 year = {2010},
  	 keywords = {Analysis of Molecular Variance (AMOVA), ascomycete, Drechslera campanulata, genetic diversity, internal transcribed spacer (ITS), invasive species, ribosomal RNA},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Mycologia},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {We examined genetic variation in the ascomycete pathogen Pyrenophora semeniperda cultured from seeds of the invasive grass Bromus tectorum in the Intermountain West of North America. We sequenced the internal transcribed spacer (ITS) region of the nuclear ribosomal RNA genome in 417 monoconidial cultures collected from 20 sites in Washington, Idaho, Utah, and Colorado, USA. ITS sequence diversity was surprisingly high; 12 unique haplotypes were identified, averaging 1.3%  pairwise sequence divergence. All sites had at least two haplotypes present, and three sites had seven or more. One haplotype comprised 60% of the isolates and occurred at all 20 locations; the remaining haplotypes generally occurred at low frequencies within sites, but at multiple sites throughout the region. Sites in Washington and Idaho were more diverse than those in Utah and Colorado, averaging 2 more haplotypes and 67% more pairwise differences among haplotypes at a site. Analysis of Molecular Variance (AMOVA) indicated that more than 80% of the genetic variation is found within sampling locations, while 7 ? 11% of the variation can be attributed to differences between northern (Washington and Idaho) and southern (Utah and Colorado) populations. The wide distribution of even uncommon haplotypes among sampling sites, and weak correlations between genetic and geographic distances among populations (< 0.2), suggest that these populations were recently established from a common source. We hypothesize that the strains of P. semeniperda infecting B. tectorum in western North America probably arrived with the invasive grass from its native Eurasian range. }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 18858
  AU  - Boose,David L.
  AU  - Harrison,Steven 
  AU  - Clement,Suzette 
  AU  - Meyer,Susan 
  T1  - Population genetic structure of the seed pathogen Pyrenophora semeniperda on Bromus tectorum in western North America
  PY  - 2010
  KW  - Analysis of Molecular Variance (AMOVA)
  KW  - ascomycete
  KW  - Drechslera campanulata
  KW  - genetic diversity
  KW  - internal transcribed spacer (ITS)
  KW  - invasive species
  KW  - ribosomal RNA
  UR  - http://dx.doi.org/
  N2  - We examined genetic variation in the ascomycete pathogen Pyrenophora semeniperda cultured from seeds of the invasive grass Bromus tectorum in the Intermountain West of North America. We sequenced the internal transcribed spacer (ITS) region of the nuclear ribosomal RNA genome in 417 monoconidial cultures collected from 20 sites in Washington, Idaho, Utah, and Colorado, USA. ITS sequence diversity was surprisingly high; 12 unique haplotypes were identified, averaging 1.3%  pairwise sequence divergence. All sites had at least two haplotypes present, and three sites had seven or more. One haplotype comprised 60% of the isolates and occurred at all 20 locations; the remaining haplotypes generally occurred at low frequencies within sites, but at multiple sites throughout the region. Sites in Washington and Idaho were more diverse than those in Utah and Colorado, averaging 2 more haplotypes and 67% more pairwise differences among haplotypes at a site. Analysis of Molecular Variance (AMOVA) indicated that more than 80% of the genetic variation is found within sampling locations, while 7 ? 11% of the variation can be attributed to differences between northern (Washington and Idaho) and southern (Utah and Colorado) populations. The wide distribution of even uncommon haplotypes among sampling sites, and weak correlations between genetic and geographic distances among populations (< 0.2), suggest that these populations were recently established from a common source. We hypothesize that the strains of P. semeniperda infecting B. tectorum in western North America probably arrived with the invasive grass from its native Eurasian range. 
  L3  - 
  JF  - Mycologia
  VL  - 
  IS  - 
  ER  -