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Citation for Study 14609

Citation title: "Two clonal lineages of Phytophthora citrophthora from citrus in South Africa represent a single phylogenetic species".

Study name: "Two clonal lineages of Phytophthora citrophthora from citrus in South Africa represent a single phylogenetic species".

This study is part of submission 14609 (Status: Published).

Citation

Spies C.F., Meitz-hopkins J.C., Langenhoven S.D., Pretorius M.C., & Mcleod A. 2014. Two clonal lineages of Phytophthora citrophthora from citrus in South Africa represent a single phylogenetic species. Mycologia, .

Authors

• Spies C.F. (submitter) +27218874690
• Meitz-hopkins J.C.
• Langenhoven S.D.
• Pretorius M.C.
• Mcleod A.

Abstract

Phytophthora citrophthora, a serious pathogen of citrus, is comprised of distinct clonal lineages on citrus in Eastern Corsica and Spain, with the G1 lineage dominating. In South Africa, the extent of genetic variation among citrus associated P. citrophthora isolates is unknown. This was therefore investigated using isolates from South Africa (n=60), Spain (n=10), and six reference isolates representing three P. citrophthora groups CTR1, CTR2 and CTR3 previously identified by Mchau & Coffey (1994) using isozyme polymorphisms. Isolates from South Africa and Spain belonged to two lineages (G1 and G2) based on an internal transcribed spacer (ITS) phylogeny and random amplified microsatellites (RAMS) and random amplified polymorphic DNA (RAPD) profiling. Although the combined RAMS and RAPD data identified 14 genotypes among these isolates, unweighted pair group method with arithmetic mean (UPGMA) analyses grouped the isolates into two distinct clusters that corresponded to the G1 and G2 lineages. The G1 lineage predominated among isolates from South Africa (92 %) and Spain (100 %). Phylogenetic analyses of the ?-tubulin, cytochrome c oxidase subunit I (COX1) and ITS regions did not support the hypothesis that the two lineages represent distinct phylogenetic species, but suggested that isozyme group CTR2 and possibly CTR3 should be redescribed as species distinct from P. citrophthora (South African, Spanish and CTR1 reference isolates). Mating type analyses, using tester strains from groups CTR2 and CTR3 revealed that most G1 isolates were sterile (n = 57) but that a few were of the A1 mating type (n = 8), whereas all G2 isolates were A2 mating type (n = 5). The mating type designation was confirmed using P. capsici tester strains. However, when the A1 (G1 lineage) and A2 (G2 lineage including CTR1 reference isolates) mating type isolates were paired in all possible combinations with each other, no oogonia or antheridia were produced. This suggests that only the tester P. capsici, CTR2 and CTR3 strains were able to produce sexual structures and that the G1 and G2 lineages are sterile and reproductively isolated, which is supported by the molecular data.

Keywords

intraspecific variation, phylogeny, RAMS, RAPDs

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S14609
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref22295,
  	 author = {Chris Frederik Jakobus Spies and Julia C. Meitz-Hopkins and Shaun D. Langenhoven and M. C. Pretorius and Adele  McLeod},
  	 title = {Two clonal lineages of Phytophthora citrophthora from citrus in South Africa represent a single phylogenetic species},
  	 year = {2014},
  	 keywords = {intraspecific variation, phylogeny, RAMS, RAPDs},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Mycologia},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Phytophthora citrophthora, a serious pathogen of citrus, is comprised of distinct clonal lineages on citrus in Eastern Corsica and Spain, with the G1 lineage dominating.  In South Africa, the extent of genetic variation among citrus associated P. citrophthora isolates is unknown. This was therefore investigated using isolates from South Africa (n=60), Spain (n=10), and six reference isolates representing three P. citrophthora groups CTR1, CTR2 and CTR3 previously identified by Mchau & Coffey (1994) using isozyme polymorphisms.  Isolates from South Africa and Spain belonged to two lineages (G1 and G2) based on an internal transcribed spacer (ITS) phylogeny and random amplified microsatellites (RAMS) and random amplified polymorphic DNA (RAPD) profiling.  Although the combined RAMS and RAPD data identified 14 genotypes among these isolates, unweighted pair group method with arithmetic mean (UPGMA) analyses grouped the isolates into two distinct clusters that corresponded to the G1 and G2 lineages. The G1 lineage predominated among isolates from South Africa (92 %) and Spain (100 %). Phylogenetic analyses of the ?-tubulin, cytochrome c oxidase subunit I (COX1) and ITS regions did not support the hypothesis that the two lineages represent distinct phylogenetic species, but suggested that isozyme group CTR2 and possibly CTR3 should be redescribed as species distinct from P. citrophthora (South African, Spanish and CTR1 reference isolates). Mating type analyses, using tester strains from groups CTR2 and CTR3 revealed that most G1 isolates were sterile (n = 57) but that a few were of the A1 mating type (n = 8), whereas all G2 isolates were A2 mating type (n = 5). The mating type designation was confirmed using P. capsici tester strains. However, when the A1 (G1 lineage) and A2 (G2 lineage including CTR1 reference isolates) mating type isolates were paired in all possible combinations with each other, no oogonia or antheridia were produced.  This suggests that only the tester P. capsici, CTR2 and CTR3 strains were able to produce sexual structures and that the G1 and G2 lineages are sterile and reproductively isolated, which is supported by the molecular data.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 22295
  AU  - Spies,Chris Frederik Jakobus
  AU  - Meitz-Hopkins,Julia C.
  AU  - Langenhoven,Shaun D.
  AU  - Pretorius,M. C.
  AU  - McLeod,Adele 
  T1  - Two clonal lineages of Phytophthora citrophthora from citrus in South Africa represent a single phylogenetic species
  PY  - 2014
  KW  - intraspecific variation
  KW  - phylogeny
  KW  - RAMS
  KW  - RAPDs
  UR  - http://dx.doi.org/
  N2  - Phytophthora citrophthora, a serious pathogen of citrus, is comprised of distinct clonal lineages on citrus in Eastern Corsica and Spain, with the G1 lineage dominating.  In South Africa, the extent of genetic variation among citrus associated P. citrophthora isolates is unknown. This was therefore investigated using isolates from South Africa (n=60), Spain (n=10), and six reference isolates representing three P. citrophthora groups CTR1, CTR2 and CTR3 previously identified by Mchau & Coffey (1994) using isozyme polymorphisms.  Isolates from South Africa and Spain belonged to two lineages (G1 and G2) based on an internal transcribed spacer (ITS) phylogeny and random amplified microsatellites (RAMS) and random amplified polymorphic DNA (RAPD) profiling.  Although the combined RAMS and RAPD data identified 14 genotypes among these isolates, unweighted pair group method with arithmetic mean (UPGMA) analyses grouped the isolates into two distinct clusters that corresponded to the G1 and G2 lineages. The G1 lineage predominated among isolates from South Africa (92 %) and Spain (100 %). Phylogenetic analyses of the ?-tubulin, cytochrome c oxidase subunit I (COX1) and ITS regions did not support the hypothesis that the two lineages represent distinct phylogenetic species, but suggested that isozyme group CTR2 and possibly CTR3 should be redescribed as species distinct from P. citrophthora (South African, Spanish and CTR1 reference isolates). Mating type analyses, using tester strains from groups CTR2 and CTR3 revealed that most G1 isolates were sterile (n = 57) but that a few were of the A1 mating type (n = 8), whereas all G2 isolates were A2 mating type (n = 5). The mating type designation was confirmed using P. capsici tester strains. However, when the A1 (G1 lineage) and A2 (G2 lineage including CTR1 reference isolates) mating type isolates were paired in all possible combinations with each other, no oogonia or antheridia were produced.  This suggests that only the tester P. capsici, CTR2 and CTR3 strains were able to produce sexual structures and that the G1 and G2 lineages are sterile and reproductively isolated, which is supported by the molecular data.
  L3  - 
  JF  - Mycologia
  VL  - 
  IS  - 
  ER  -