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Citation for Study 10199

Citation title: "Multigene phylogenies reveal that red band needle blight of Pinus is caused by two distinct species of Dothistroma, D. septosporum and D. pini".

This study was previously identified under the legacy study ID S1209 (Status: Published).

Citation

Barnes I., Crous P.W., Wingfield B.D., & Wingfield M.J. 2004. Multigene phylogenies reveal that red band needle blight of Pinus is caused by two distinct species of Dothistroma, D. septosporum and D. pini. Studies in Mycology, 50(2): 551-565.

Authors

• Barnes I.
• Crous P.W.
• Wingfield B.D.
• Wingfield M.J.

Abstract

The red band needle blight fungus, Dothistroma septosporum is a widely distributed pathogen of many pine species. Three morphological varieties of this pathogen have been described based on differences in conidial length. However, controversy exists as to whether spore size represents an adequate characteristic to distinguish between forms of D. septosporum. The aim of this investigation was to consider the phylogenetic relationships between D. septosporum isolates from different countries. An additional objective was to determine whether comparisons of DNA sequence data support the morphological varieties recognized for this species. DNA from portions of the nuclear ribosomal internal transcribed spacer (ITS), ?-tubulin and elongation factor 1-? genes were sequenced and analysed for isolates from 13 different countries representing five continents. Results show that isolates of the pathogen encompasses two divergent lineages representing distinct phylogenetic species. One phylogenetic species (Lineage I) is found worldwide, while the other (Lineage II), is restricted to the North-Central U.S.A. The names D. pini and D. septosporum are available for these species. The former name should apply to the phylogenetic species currently known only from the United States. The latter fungus has a worldwide distribution and is the causal agent of the serious disease known as red band needle blight that has damaged exotic plantations of Pinus radiata in the Southern Hemisphere. A PCR-restriction fragment length polymorphism (RFLP) diagnostic protocol is described that distinguishes between all the currently known Dothistroma species. The previous classification of D. septosporum isolates into different varieties based on morphology is inconsistent and not supported by our DNA analyses. We therefore reject further use of varietal names in Dothistroma.

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S10199
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref18690,
  	 author = {Irene  Barnes and Pedro W. Crous and Brenda D Wingfield and Michael J Wingfield},
  	 title = {Multigene phylogenies reveal that red band needle blight of Pinus is caused by two distinct species of Dothistroma, D. septosporum and D. pini},
  	 year = {2004},
  	 keywords = {},
  	 doi = {},
  	 url = {},
  	 pmid = {},
  	 journal = {Studies in Mycology},
  	 volume = {50},
  	 number = {2},
  	 pages = {551--565},
  	 abstract = {The red band needle blight fungus, Dothistroma septosporum is a widely distributed pathogen of many pine species. Three morphological varieties of this pathogen have been described based on differences in conidial length. However, controversy exists as to whether spore size represents an adequate characteristic to distinguish between forms of D. septosporum. The aim of this investigation was to consider the phylogenetic relationships between D. septosporum isolates from different countries. An additional objective was to determine whether comparisons of DNA sequence data support the morphological varieties recognized for this species. DNA from portions of the nuclear ribosomal internal transcribed spacer (ITS), ?-tubulin and elongation factor 1-? genes were sequenced and analysed for isolates from 13 different countries representing five continents. Results show that isolates of the pathogen encompasses two divergent lineages representing distinct phylogenetic species. One phylogenetic species (Lineage I) is found worldwide, while the other (Lineage II), is restricted to the North-Central U.S.A. The names D. pini and D. septosporum are available for these species. The former name should apply to the phylogenetic species currently known only from the United States. The latter fungus has a worldwide distribution and is the causal agent of the serious disease known as red band needle blight that has damaged exotic plantations of Pinus radiata in the Southern Hemisphere. A PCR-restriction fragment length polymorphism (RFLP) diagnostic protocol is described that distinguishes between all the currently known Dothistroma species. The previous classification of D. septosporum isolates into different varieties based on morphology is inconsistent and not supported by our DNA analyses. We therefore reject further use of varietal names in Dothistroma.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 18690
  AU  - Barnes,Irene 
  AU  - Crous,Pedro W.
  AU  - Wingfield,Brenda D
  AU  - Wingfield,Michael J
  T1  - Multigene phylogenies reveal that red band needle blight of Pinus is caused by two distinct species of Dothistroma, D. septosporum and D. pini
  PY  - 2004
  UR  - 
  N2  - The red band needle blight fungus, Dothistroma septosporum is a widely distributed pathogen of many pine species. Three morphological varieties of this pathogen have been described based on differences in conidial length. However, controversy exists as to whether spore size represents an adequate characteristic to distinguish between forms of D. septosporum. The aim of this investigation was to consider the phylogenetic relationships between D. septosporum isolates from different countries. An additional objective was to determine whether comparisons of DNA sequence data support the morphological varieties recognized for this species. DNA from portions of the nuclear ribosomal internal transcribed spacer (ITS), ?-tubulin and elongation factor 1-? genes were sequenced and analysed for isolates from 13 different countries representing five continents. Results show that isolates of the pathogen encompasses two divergent lineages representing distinct phylogenetic species. One phylogenetic species (Lineage I) is found worldwide, while the other (Lineage II), is restricted to the North-Central U.S.A. The names D. pini and D. septosporum are available for these species. The former name should apply to the phylogenetic species currently known only from the United States. The latter fungus has a worldwide distribution and is the causal agent of the serious disease known as red band needle blight that has damaged exotic plantations of Pinus radiata in the Southern Hemisphere. A PCR-restriction fragment length polymorphism (RFLP) diagnostic protocol is described that distinguishes between all the currently known Dothistroma species. The previous classification of D. septosporum isolates into different varieties based on morphology is inconsistent and not supported by our DNA analyses. We therefore reject further use of varietal names in Dothistroma.
  L3  - 
  JF  - Studies in Mycology
  VL  - 50
  IS  - 2
  SP  - 551
  EP  - 565
  ER  -