@ARTICLE{TreeBASE2Ref23854,
author = {Wilson Frantine-Silva and Silvia H. Sofia and Mário L. Orsi and Fernanda S. Almeida},
title = {DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring},
year = {2015},
keywords = {Ichthyoplankton, fish recruitment, conservation, upper Paraná River},
doi = { 10.1111/1755-0998.12385},
url = {http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12385/abstract},
pmid = {},
journal = {Molecular Ecology Resources},
volume = {},
number = {},
pages = {},
abstract = {Quantifying and classifying ichthyoplankton is one of the most effective ways of monitoring the recruitment process in fish. However, correctly identifying the fish based on morphological characters is extremely difficult, especially in the early stages of development. We examined ichthyoplankton from tributaries and reservoirs along the middle stretch of the Paranapanema River, one of the areas most impacted by hydroelectric projects in the Neotropics. Matching DNA sequences of the COI gene (628-648 bp) allowed us to identify 99.25% of 536 samples of eggs (293) and larvae (243) subjected to BOLD-IDS similarity analysis with a species-level threshold of 1.3%. The results revealed 37 species in 27 genera, 15 families and four orders, some 23.8% of documented fish species in the Paranapanema River. Molecular identification meant that we could include data from egg samples that accounted for about 30% of the species richness observed. The results in this study confirm the efficacy of DNA barcoding in identifying Neotropical ichthyoplankton and show how the data produced provide valuable information for preparing plans for conserving and managing inland waters.}
}
Citation for Study 16630
Citation title:
"DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring".
Study name:
"DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring".
This study is part of submission 16630
(Status: Published).
Citation
Frantine-silva W., Sofia S.H., Orsi M.L., & Almeida F.S. 2015. DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring. Molecular Ecology Resources, .
Authors
-
Frantine-silva W.
(submitter)
554399290927
-
Sofia S.H.
-
Orsi M.L.
-
Almeida F.S.
Abstract
Quantifying and classifying ichthyoplankton is one of the most effective ways of monitoring the recruitment process in fish. However, correctly identifying the fish based on morphological characters is extremely difficult, especially in the early stages of development. We examined ichthyoplankton from tributaries and reservoirs along the middle stretch of the Paranapanema River, one of the areas most impacted by hydroelectric projects in the Neotropics. Matching DNA sequences of the COI gene (628-648 bp) allowed us to identify 99.25% of 536 samples of eggs (293) and larvae (243) subjected to BOLD-IDS similarity analysis with a species-level threshold of 1.3%. The results revealed 37 species in 27 genera, 15 families and four orders, some 23.8% of documented fish species in the Paranapanema River. Molecular identification meant that we could include data from egg samples that accounted for about 30% of the species richness observed. The results in this study confirm the efficacy of DNA barcoding in identifying Neotropical ichthyoplankton and show how the data produced provide valuable information for preparing plans for conserving and managing inland waters.
Keywords
Ichthyoplankton, fish recruitment, conservation, upper Paraná River
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S16630
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref23854,
author = {Wilson Frantine-Silva and Silvia H. Sofia and Mário L. Orsi and Fernanda S. Almeida},
title = {DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring},
year = {2015},
keywords = {Ichthyoplankton, fish recruitment, conservation, upper Paraná River},
doi = { 10.1111/1755-0998.12385},
url = {http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12385/abstract},
pmid = {},
journal = {Molecular Ecology Resources},
volume = {},
number = {},
pages = {},
abstract = {Quantifying and classifying ichthyoplankton is one of the most effective ways of monitoring the recruitment process in fish. However, correctly identifying the fish based on morphological characters is extremely difficult, especially in the early stages of development. We examined ichthyoplankton from tributaries and reservoirs along the middle stretch of the Paranapanema River, one of the areas most impacted by hydroelectric projects in the Neotropics. Matching DNA sequences of the COI gene (628-648 bp) allowed us to identify 99.25% of 536 samples of eggs (293) and larvae (243) subjected to BOLD-IDS similarity analysis with a species-level threshold of 1.3%. The results revealed 37 species in 27 genera, 15 families and four orders, some 23.8% of documented fish species in the Paranapanema River. Molecular identification meant that we could include data from egg samples that accounted for about 30% of the species richness observed. The results in this study confirm the efficacy of DNA barcoding in identifying Neotropical ichthyoplankton and show how the data produced provide valuable information for preparing plans for conserving and managing inland waters.}
}
- Show RIS reference
TY - JOUR
ID - 23854
AU - Frantine-Silva,Wilson
AU - Sofia,Silvia H.
AU - Orsi,Mário L.
AU - Almeida,Fernanda S.
T1 - DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring
PY - 2015
KW - Ichthyoplankton
KW - fish recruitment
KW - conservation
KW - upper Paraná River
UR - http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12385/abstract
N2 - Quantifying and classifying ichthyoplankton is one of the most effective ways of monitoring the recruitment process in fish. However, correctly identifying the fish based on morphological characters is extremely difficult, especially in the early stages of development. We examined ichthyoplankton from tributaries and reservoirs along the middle stretch of the Paranapanema River, one of the areas most impacted by hydroelectric projects in the Neotropics. Matching DNA sequences of the COI gene (628-648 bp) allowed us to identify 99.25% of 536 samples of eggs (293) and larvae (243) subjected to BOLD-IDS similarity analysis with a species-level threshold of 1.3%. The results revealed 37 species in 27 genera, 15 families and four orders, some 23.8% of documented fish species in the Paranapanema River. Molecular identification meant that we could include data from egg samples that accounted for about 30% of the species richness observed. The results in this study confirm the efficacy of DNA barcoding in identifying Neotropical ichthyoplankton and show how the data produced provide valuable information for preparing plans for conserving and managing inland waters.
L3 - 10.1111/1755-0998.12385
JF - Molecular Ecology Resources
VL -
IS -
ER -