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Citation for Study 23745

Citation title: "Evaluation of ITS2 molecular morphometrics effectiveness in species delimitation of Ascomycota ? a pilot study".

Study name: "Evaluation of ITS2 molecular morphometrics effectiveness in species delimitation of Ascomycota ? a pilot study".

This study is part of submission 23745 (Status: Published).

Citation

Sundaresan N., Sahu A.K., Jagan E., & Pandi M. 2018. Evaluation of ITS2 molecular morphometrics effectiveness in species delimitation of Ascomycota ? a pilot study. Fungal Biology, .

Authors

• Sundaresan N.
• Sahu A.K.
• Jagan E.
• Pandi M. (submitter)

Abstract

Exploring the secondary structure information of nuclear ribosomal internal transcribed spacer 2 (ITS2) has been a promising approach in species delimitation. However, Compensatory base changes (CBC) concept employed in this approach turns futile when CBC is absent. This prompted us to investigate the utility of insertion/deletion (INDELs) and substitutions in fungal delineation at species level. Upon this rationale, 116 strains representing 97 species, belonging to 6 genera of Ascomycota were retrieved from Q-bank for molecular morphometric analysis. CBC, INDELs and substitutions between the species of their respective genus were recorded. Most species combinations lacked CBC. Among the substitution events, transitions were predominant. INDELs were less frequent than the substitutions. These evolutionary events were mapped upon the helices to discern species specific variation sites. In 68 species unique variation sites were recognised. The remaining 29 species shared absolute similarity with distinctly named species. The variation sites catalogued in them overlapped with other distinct species and resulted in blurring of the species boundaries. Species specific variation sites recognized in this study are the preliminary results and they could be discerned with absolute confidence when larger datasets encompassing all described species of genera were investigated. They could be of potential use in barcoding fungi at species level. This study also concludes that the ITS2 molecular morphometric analysis is an efficient third dimensional study of the fungal species delimitation. This may help to avoid the false positives in species delimitations and to alleviate the challenges in molecular characterization.

Keywords

Molecular morphometrics; ITS2; CBC; INDELs; Species delimitation.

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S23745
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref29141,
  	 author = {Natesan  Sundaresan and Amit  Kumar  Sahu and Enthai Ganeshan  Jagan and Mohan  Pandi},
  	 title = {Evaluation of ITS2 molecular morphometrics effectiveness in species delimitation of Ascomycota ? a pilot study},
  	 year = {2018},
  	 keywords = {Molecular morphometrics; ITS2; CBC; INDELs; Species delimitation.},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Fungal Biology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Exploring the secondary structure information of nuclear ribosomal internal transcribed spacer 2 (ITS2) has been a promising approach in species delimitation. However, Compensatory base changes (CBC) concept employed in this approach turns futile when CBC is absent. This prompted us to investigate the utility of insertion/deletion (INDELs) and substitutions in fungal delineation at species level. Upon this rationale, 116 strains representing 97 species, belonging to 6 genera of Ascomycota were retrieved from Q-bank for molecular morphometric analysis. CBC, INDELs and substitutions between the species of their respective genus were recorded. Most species combinations lacked CBC. Among the substitution events, transitions were predominant. INDELs were less frequent than the substitutions. These evolutionary events were mapped upon the helices to discern species specific variation sites. In 68 species unique variation sites were recognised. The remaining 29 species shared absolute similarity with distinctly named species. The variation sites catalogued in them overlapped with other distinct species and resulted in blurring of the species boundaries. Species specific variation sites recognized in this study are the preliminary results and they could be discerned with absolute confidence when larger datasets encompassing all described species of genera were investigated. They could be of potential use in barcoding fungi at species level. This study also concludes that the ITS2 molecular morphometric analysis is an efficient third dimensional study of the fungal species delimitation. This may help to avoid the false positives in species delimitations and to alleviate the challenges in molecular characterization.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 29141
  AU  - Sundaresan,Natesan 
  AU  - Sahu,Amit  Kumar 
  AU  - Jagan,Enthai Ganeshan 
  AU  - Pandi,Mohan 
  T1  - Evaluation of ITS2 molecular morphometrics effectiveness in species delimitation of Ascomycota ? a pilot study
  PY  - 2018
  KW  - Molecular morphometrics; ITS2; CBC; INDELs; Species delimitation.
  UR  - http://dx.doi.org/
  N2  - Exploring the secondary structure information of nuclear ribosomal internal transcribed spacer 2 (ITS2) has been a promising approach in species delimitation. However, Compensatory base changes (CBC) concept employed in this approach turns futile when CBC is absent. This prompted us to investigate the utility of insertion/deletion (INDELs) and substitutions in fungal delineation at species level. Upon this rationale, 116 strains representing 97 species, belonging to 6 genera of Ascomycota were retrieved from Q-bank for molecular morphometric analysis. CBC, INDELs and substitutions between the species of their respective genus were recorded. Most species combinations lacked CBC. Among the substitution events, transitions were predominant. INDELs were less frequent than the substitutions. These evolutionary events were mapped upon the helices to discern species specific variation sites. In 68 species unique variation sites were recognised. The remaining 29 species shared absolute similarity with distinctly named species. The variation sites catalogued in them overlapped with other distinct species and resulted in blurring of the species boundaries. Species specific variation sites recognized in this study are the preliminary results and they could be discerned with absolute confidence when larger datasets encompassing all described species of genera were investigated. They could be of potential use in barcoding fungi at species level. This study also concludes that the ITS2 molecular morphometric analysis is an efficient third dimensional study of the fungal species delimitation. This may help to avoid the false positives in species delimitations and to alleviate the challenges in molecular characterization.
  L3  - 
  JF  - Fungal Biology
  VL  - 
  IS  - 
  ER  -