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Citation for Study 24445

Citation title: "Evaluation of the rust fungus Puccinia rapipes for biological control of Lycium ferocissimum (African boxthorn) in Australia: life cycle, taxonomy and pathogenicity".

Study name: "Evaluation of the rust fungus Puccinia rapipes for biological control of Lycium ferocissimum (African boxthorn) in Australia: life cycle, taxonomy and pathogenicity".

This study is part of submission 24445 (Status: Published).

Citation

Ireland K.B., Hunter G.C., Wood A., Delaisse C., & Morin L. 2019. Evaluation of the rust fungus Puccinia rapipes for biological control of Lycium ferocissimum (African boxthorn) in Australia: life cycle, taxonomy and pathogenicity. Fungal Biology, .

Authors

• Ireland K.B. 0262183445
• Hunter G.C. (submitter) +61 (02) 6218 3658
• Wood A.
• Delaisse C.
• Morin L.

Abstract

Fungal plant pathogens are playing an increasing role as effective, safe agents in classical weed biological control programs worldwide. Suitability of the rust fungus Puccinia rapipes as a classical biological control agent for Lycium ferocissimum (African boxthorn) in Australia was assessed using a streamlined agent selection framework. Studies with P. rapipes were undertaken to elucidate its life cycle, confirm its taxonomic placement and determine its pathogenicity to L. ferocissimum and seven closely-related Solanaceae species that occur in Australia. Field surveys in the native range of South Africa, experiments in a containment facility in Australia and DNA sequencing confirmed that P. rapipes is macrocyclic and autoecious, producing all five spore stages on L. ferocissimum. The stages not previously encountered, spermogonia and aecia, are described. Sequencing also confirmed that P. rapipes is sister to Puccinia afra, in the ?Old World Lineage? of Puccinia species on Lycieae. Two purified isolates of the fungus, representing the Eastern and Western Cape distributions of P. rapipes in South Africa, were cultured in the containment facility for use in pathogenicity testing. Lycium ferocissimum and all of the Lycium species of Eurasian origin tested ‒ L. barbarum (goji berry), L. chinense (goji berry ?chinense?) and L. ruthenicum (black goji berry) ? were susceptible to both isolates of P. rapipes. The Australian native Lycium australe and three more distantly related species in different genera tested were resistant to both isolates. The isolate from the Western Cape was significantly more pathogenic on L. ferocissimum from Australia, than the Eastern Cape isolate. Our results indicate that P. rapipes may be sufficiently host specific to pursue as a biological control agent in an Australian context, should regulators be willing to accept damage to the Eurasian goji berries being cultivated, albeit to a limited extent, in Australia.

Keywords

classical biocontrol, plant pathogen, host specificity, Lycieae

External links

• DOI: 10.1016/j.funbio.2019.08.007

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S24445
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref29613,
  	 author = {Kylie Belle Ireland and Gavin Craig Hunter and Alan R  Wood and Caroline  Delaisse and Louise  Morin},
  	 title = {Evaluation of the rust fungus Puccinia rapipes for biological control of Lycium ferocissimum (African boxthorn) in Australia: life cycle, taxonomy and pathogenicity},
  	 year = {2019},
  	 keywords = {classical biocontrol, plant pathogen, host specificity, Lycieae},
  	 doi = {10.1016/j.funbio.2019.08.007},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Fungal Biology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Fungal plant pathogens are playing an increasing role as effective, safe agents in classical weed biological control programs worldwide. Suitability of the rust fungus Puccinia rapipes as a classical biological control agent for Lycium ferocissimum (African boxthorn) in Australia was assessed using a streamlined agent selection framework. Studies with P. rapipes were undertaken to elucidate its life cycle, confirm its taxonomic placement and determine its pathogenicity to L. ferocissimum and seven closely-related Solanaceae species that occur in Australia. Field surveys in the native range of South Africa, experiments in a containment facility in Australia and DNA sequencing confirmed that P. rapipes is macrocyclic and autoecious, producing all five spore stages on L. ferocissimum. The stages not previously encountered, spermogonia and aecia, are described. Sequencing also confirmed that P. rapipes is sister to Puccinia afra, in the ?Old World Lineage? of Puccinia species on Lycieae. Two purified isolates of the fungus, representing the Eastern and Western Cape distributions of P. rapipes in South Africa, were cultured in the containment facility for use in pathogenicity testing. Lycium ferocissimum and all of the Lycium species of Eurasian origin tested ‒ L. barbarum (goji berry), L. chinense (goji berry ?chinense?) and L. ruthenicum (black goji berry) ? were susceptible to both isolates of P. rapipes. The Australian native Lycium australe and three more distantly related species in different genera tested were resistant to both isolates. The isolate from the Western Cape was significantly more pathogenic on L. ferocissimum from Australia, than the Eastern Cape isolate. Our results indicate that P. rapipes may be sufficiently host specific to pursue as a biological control agent in an Australian context, should regulators be willing to accept damage to the Eurasian goji berries being cultivated, albeit to a limited extent, in Australia.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 29613
  AU  - Ireland,Kylie Belle
  AU  - Hunter,Gavin Craig
  AU  - Wood,Alan R 
  AU  - Delaisse,Caroline 
  AU  - Morin,Louise 
  T1  - Evaluation of the rust fungus Puccinia rapipes for biological control of Lycium ferocissimum (African boxthorn) in Australia: life cycle, taxonomy and pathogenicity
  PY  - 2019
  KW  - classical biocontrol
  KW  - plant pathogen
  KW  - host specificity
  KW  - Lycieae
  UR  - http://dx.doi.org/10.1016/j.funbio.2019.08.007
  N2  - Fungal plant pathogens are playing an increasing role as effective, safe agents in classical weed biological control programs worldwide. Suitability of the rust fungus Puccinia rapipes as a classical biological control agent for Lycium ferocissimum (African boxthorn) in Australia was assessed using a streamlined agent selection framework. Studies with P. rapipes were undertaken to elucidate its life cycle, confirm its taxonomic placement and determine its pathogenicity to L. ferocissimum and seven closely-related Solanaceae species that occur in Australia. Field surveys in the native range of South Africa, experiments in a containment facility in Australia and DNA sequencing confirmed that P. rapipes is macrocyclic and autoecious, producing all five spore stages on L. ferocissimum. The stages not previously encountered, spermogonia and aecia, are described. Sequencing also confirmed that P. rapipes is sister to Puccinia afra, in the ?Old World Lineage? of Puccinia species on Lycieae. Two purified isolates of the fungus, representing the Eastern and Western Cape distributions of P. rapipes in South Africa, were cultured in the containment facility for use in pathogenicity testing. Lycium ferocissimum and all of the Lycium species of Eurasian origin tested ‒ L. barbarum (goji berry), L. chinense (goji berry ?chinense?) and L. ruthenicum (black goji berry) ? were susceptible to both isolates of P. rapipes. The Australian native Lycium australe and three more distantly related species in different genera tested were resistant to both isolates. The isolate from the Western Cape was significantly more pathogenic on L. ferocissimum from Australia, than the Eastern Cape isolate. Our results indicate that P. rapipes may be sufficiently host specific to pursue as a biological control agent in an Australian context, should regulators be willing to accept damage to the Eurasian goji berries being cultivated, albeit to a limited extent, in Australia.
  L3  - 10.1016/j.funbio.2019.08.007
  JF  - Fungal Biology
  VL  - 
  IS  - 
  ER  -