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Citation for Study 11370

Citation title: "Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot".

Study name: "Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot".

This study is part of submission 11360 (Status: Published).

Citation

Glienke C., Cunnington J., Shivas R., Groenewald J.Z., & Crous P.W. 2011. Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot. Persoonia, 26: 47-56.

Authors

• Glienke C.
• Cunnington J.
• Shivas R.
• Groenewald J.Z. (submitter) +31302122600
• Crous P.W.

Abstract

We investigated the identity and genetic diversity of more than 100 isolates belonging to Phyllosticta (teleomorph Guignardia), with particular emphasis on Phyllosticta citricarpa and Guignardia mangiferae s.l. occurring on Citrus. Phyllosticta citricarpa is the causal agent of Citrus Black Spot and is subject to phytosanitary legislation in the EU. This species is frequently confused with a taxon generally referred to as G. mangiferae, the presumed teleomorph of P. capitalensis, which is a non-pathogenic endophyte, commonly isolated from citrus leaves and fruits and a wide range of other hosts. DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S nrDNA, ITS2) and partial translation elongation factor 1-alpha (TEF1), actin and glyceraldehyde-3-phosphate dehydrogenase (GPDH) genes resolved nine clades correlating to seven known, and two apparently undescribed species. Phyllosticta citribraziliensis is newly described as an endophytic species occurring on Citrus in Brazil. An epitype is designated for P. citricarpa from material newly collected in Australia, which is distinct from P. citriasiana, presently only known on C. maxima from Asia. Phyllosticta bifrenariae is newly described for a species causing leaf and bulb spots on Bifrenaria harrisoniae (Orchidaceae) in Brazil. It is morphologically distinct from P. capitalensis, which was originally described from Stanhopea (Orchidaceae) in Brazil; an epitype is designated here. Guignardia mangiferae, which was originally described from Mangifera indica (Anacardiaceae) in India, is distinguished from the non-pathogenic endophyte, P. brazilianiae sp. nov., which is common on M. indica in Brazil. Furthermore, a combined phylogenetic tree revealed the P. capitalensis s.l. clade to be genetically distinct from the reference isolate of G. mangiferae. Several names are available for this clade, the oldest being P. capitalensis. These results suggest that endophytic, non-pathogenic isolates occurring on a wide host range would be more correctly referred to as P. capitalensis. However, more genes need to be analysed to fully resolve the morphological variation still observed within this clade.

Keywords

Guignardia endophyllicola, Guignardia mangiferae, Phyllosticta bifrenariae, Phyllosticta brazilianiae, Phyllosticta capitalensis, Phyllosticta citriasiana, Phyllosticta citribraziliensis, Phyllosticta citricarpa, taxonomy

External links

• DOI: 10.3767/003158511X569169
• Other URL: http://www.persoonia.org

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S11370
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref19597,
  	 author = {Chirlei  Glienke and James H.  Cunnington and Roger  Shivas and  Johannes (Ewald) Zacharias Groenewald and Pedro W. Crous},
  	 title = {Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot},
  	 year = {2011},
  	 keywords = {Guignardia endophyllicola, Guignardia mangiferae, Phyllosticta bifrenariae, Phyllosticta brazilianiae, Phyllosticta capitalensis, Phyllosticta citriasiana, Phyllosticta citribraziliensis, Phyllosticta citricarpa, taxonomy},
  	 doi = {10.3767/003158511X569169},
  	 url = {http://www.persoonia.org},
  	 pmid = {},
  	 journal = {Persoonia},
  	 volume = {26},
  	 number = {},
  	 pages = {47--56},
  	 abstract = {We investigated the identity and genetic diversity of more than 100 isolates belonging to Phyllosticta (teleomorph Guignardia), with particular emphasis on Phyllosticta citricarpa and Guignardia mangiferae s.l. occurring on Citrus. Phyllosticta citricarpa is the causal agent of Citrus Black Spot and is subject to phytosanitary legislation in the EU. This species is frequently confused with a taxon generally referred to as G. mangiferae, the presumed teleomorph of P. capitalensis, which is a non-pathogenic endophyte, commonly isolated from citrus leaves and fruits and a wide range of other hosts. DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S nrDNA, ITS2) and partial translation elongation factor 1-alpha (TEF1), actin and glyceraldehyde-3-phosphate dehydrogenase (GPDH) genes resolved nine clades correlating to seven known, and two apparently undescribed species. Phyllosticta citribraziliensis is newly described as an endophytic species occurring on Citrus in Brazil. An epitype is designated for P. citricarpa from material newly collected in Australia, which is distinct from P. citriasiana, presently only known on C. maxima from Asia. Phyllosticta bifrenariae is newly described for a species causing leaf and bulb spots on Bifrenaria harrisoniae (Orchidaceae) in Brazil. It is morphologically distinct from P. capitalensis, which was originally described from Stanhopea (Orchidaceae) in Brazil; an epitype is designated here. Guignardia mangiferae, which was originally described from Mangifera indica (Anacardiaceae) in India, is distinguished from the non-pathogenic endophyte, P. brazilianiae sp. nov., which is common on M. indica in Brazil. Furthermore, a combined phylogenetic tree revealed the P. capitalensis s.l. clade to be genetically distinct from the reference isolate of G. mangiferae. Several names are available for this clade, the oldest being P. capitalensis. These results suggest that endophytic, non-pathogenic isolates occurring on a wide host range would be more correctly referred to as P. capitalensis. However, more genes need to be analysed to fully resolve the morphological variation still observed within this clade.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 19597
  AU  - Glienke,Chirlei 
  AU  - Cunnington,James H. 
  AU  - Shivas,Roger 
  AU  - Groenewald, Johannes (Ewald) Zacharias
  AU  - Crous,Pedro W.
  T1  - Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot
  PY  - 2011
  KW  - Guignardia endophyllicola
  KW  - Guignardia mangiferae
  KW  - Phyllosticta bifrenariae
  KW  - Phyllosticta brazilianiae
  KW  - Phyllosticta capitalensis
  KW  - Phyllosticta citriasiana
  KW  - Phyllosticta citribraziliensis
  KW  - Phyllosticta citricarpa
  KW  - taxonomy
  UR  - http://www.persoonia.org
  N2  - We investigated the identity and genetic diversity of more than 100 isolates belonging to Phyllosticta (teleomorph Guignardia), with particular emphasis on Phyllosticta citricarpa and Guignardia mangiferae s.l. occurring on Citrus. Phyllosticta citricarpa is the causal agent of Citrus Black Spot and is subject to phytosanitary legislation in the EU. This species is frequently confused with a taxon generally referred to as G. mangiferae, the presumed teleomorph of P. capitalensis, which is a non-pathogenic endophyte, commonly isolated from citrus leaves and fruits and a wide range of other hosts. DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S nrDNA, ITS2) and partial translation elongation factor 1-alpha (TEF1), actin and glyceraldehyde-3-phosphate dehydrogenase (GPDH) genes resolved nine clades correlating to seven known, and two apparently undescribed species. Phyllosticta citribraziliensis is newly described as an endophytic species occurring on Citrus in Brazil. An epitype is designated for P. citricarpa from material newly collected in Australia, which is distinct from P. citriasiana, presently only known on C. maxima from Asia. Phyllosticta bifrenariae is newly described for a species causing leaf and bulb spots on Bifrenaria harrisoniae (Orchidaceae) in Brazil. It is morphologically distinct from P. capitalensis, which was originally described from Stanhopea (Orchidaceae) in Brazil; an epitype is designated here. Guignardia mangiferae, which was originally described from Mangifera indica (Anacardiaceae) in India, is distinguished from the non-pathogenic endophyte, P. brazilianiae sp. nov., which is common on M. indica in Brazil. Furthermore, a combined phylogenetic tree revealed the P. capitalensis s.l. clade to be genetically distinct from the reference isolate of G. mangiferae. Several names are available for this clade, the oldest being P. capitalensis. These results suggest that endophytic, non-pathogenic isolates occurring on a wide host range would be more correctly referred to as P. capitalensis. However, more genes need to be analysed to fully resolve the morphological variation still observed within this clade.
  L3  - 10.3767/003158511X569169
  JF  - Persoonia
  VL  - 26
  IS  - 
  SP  - 47
  EP  - 56
  ER  -