CiteULike

Delicious

Connotea

Citation for Study 14902

Citation title: "DNA barcoding of Mycosphaerella species of quarantine importance to Europe".

Study name: "DNA barcoding of Mycosphaerella species of quarantine importance to Europe".

This study is part of submission 14902 (Status: Published).

Citation

Quaedvlieg W., Groenewald J.Z., De jes?s y??ez-morales M., & Crous P.W. 2013. DNA barcoding of Mycosphaerella species of quarantine importance to Europe. Persoonia, 29: 101-115.

Authors

• Quaedvlieg W. (submitter) 0628052286
• Groenewald J.Z. +31302122600
• De jes?s y??ez-morales M.
• Crous P.W.

Abstract

Abstract The EU 7th Framework Program provided funds for Quarantine Barcoding of Life (QBOL) to develop a quick, reliable and accurate DNA barcode-based diagnostic tool for selected species on the European and Mediter- ranean Plant Protection Organization (EPPO) A1/A2 quarantine lists. Seven nuclear genomic loci were evaluated to determine those best suited for identifying species of Mycosphaerella and/or its associated anamorphs. These genes included β-tubulin (Btub), internal transcribed spacer regions of the nrDNA operon (ITS), 28S nrDNA (LSU), Actin (Act), Calmodulin (Cal), Translation elongation factor 1-alpha (EF-1α) and RNA polymerase II second larg- est subunit (RPB2). Loci were tested on their Kimura-2-parameter-based inter- and intraspecific variation, PCR amplification success rate and ability to distinguish between quarantine species and closely related taxa. Results showed that none of these loci was solely suited as a reliable barcoding locus for the tested fungi. A combination of a primary and secondary barcoding locus was found to compensate for individual weaknesses and provide reliable identification. A combination of ITS with either EF-1α or Btub was reliable as barcoding loci for EPPO A1/A2-listed Mycosphaerella species. Furthermore, Lecanosticta acicola was shown to represent a species complex, revealing two novel species described here, namely L. brevispora sp. nov. on Pinus sp. from Mexico and L. guatemalensis sp. nov. on Pinus oocarpa from Guatemala. Epitypes were also designated for L. acicola and L. longispora to resolve the genetic application of these names.

External links

• DOI: 10.3767/003158512X661282
• Other URL: http://dx.doi.org/10.3767/003158512X661282

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S14902
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref22534,
  	 author = {William  Quaedvlieg and  Johannes (Ewald) Zacharias Groenewald and Mar?a  de Jes?s Y??ez-Morales and Pedro W. Crous},
  	 title = {DNA barcoding of Mycosphaerella species of quarantine importance to Europe},
  	 year = {2013},
  	 keywords = {},
  	 doi = {10.3767/003158512X661282},
  	 url = {http://dx.doi.org/10.3767/003158512X661282},
  	 pmid = {},
  	 journal = {Persoonia},
  	 volume = {29},
  	 number = {},
  	 pages = {101--115},
  	 abstract = {Abstract  The EU 7th Framework Program provided funds for Quarantine Barcoding of Life (QBOL) to develop a quick, reliable and accurate DNA barcode-based diagnostic tool for selected species on the European and Mediter- ranean Plant Protection Organization (EPPO) A1/A2 quarantine lists. Seven nuclear genomic loci were evaluated to determine those best suited for identifying species of Mycosphaerella and/or its associated anamorphs. These genes included β-tubulin (Btub), internal transcribed spacer regions of the nrDNA operon (ITS), 28S nrDNA (LSU), Actin (Act), Calmodulin (Cal), Translation elongation factor 1-alpha (EF-1α) and RNA polymerase II second larg- est subunit (RPB2). Loci were tested on their Kimura-2-parameter-based inter- and intraspecific variation, PCR amplification success rate and ability to distinguish between quarantine species and closely related taxa. Results showed that none of these loci was solely suited as a reliable barcoding locus for the tested fungi. A combination of a primary and secondary barcoding locus was found to compensate for individual weaknesses and provide reliable identification. A combination of ITS with either EF-1α or Btub was reliable as barcoding loci for EPPO A1/A2-listed Mycosphaerella species. Furthermore, Lecanosticta acicola was shown to represent a species complex, revealing two novel species described here, namely L. brevispora sp. nov. on Pinus sp. from Mexico and L. guatemalensis sp. nov. on Pinus oocarpa from Guatemala. Epitypes were also designated for L. acicola and L. longispora to resolve the genetic application of these names. }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 22534
  AU  - Quaedvlieg,William 
  AU  - Groenewald, Johannes (Ewald) Zacharias
  AU  - de Jes?s Y??ez-Morales,Mar?a 
  AU  - Crous,Pedro W.
  T1  - DNA barcoding of Mycosphaerella species of quarantine importance to Europe
  PY  - 2013
  KW  - 
  UR  - http://dx.doi.org/10.3767/003158512X661282
  N2  - Abstract  The EU 7th Framework Program provided funds for Quarantine Barcoding of Life (QBOL) to develop a quick, reliable and accurate DNA barcode-based diagnostic tool for selected species on the European and Mediter- ranean Plant Protection Organization (EPPO) A1/A2 quarantine lists. Seven nuclear genomic loci were evaluated to determine those best suited for identifying species of Mycosphaerella and/or its associated anamorphs. These genes included β-tubulin (Btub), internal transcribed spacer regions of the nrDNA operon (ITS), 28S nrDNA (LSU), Actin (Act), Calmodulin (Cal), Translation elongation factor 1-alpha (EF-1α) and RNA polymerase II second larg- est subunit (RPB2). Loci were tested on their Kimura-2-parameter-based inter- and intraspecific variation, PCR amplification success rate and ability to distinguish between quarantine species and closely related taxa. Results showed that none of these loci was solely suited as a reliable barcoding locus for the tested fungi. A combination of a primary and secondary barcoding locus was found to compensate for individual weaknesses and provide reliable identification. A combination of ITS with either EF-1α or Btub was reliable as barcoding loci for EPPO A1/A2-listed Mycosphaerella species. Furthermore, Lecanosticta acicola was shown to represent a species complex, revealing two novel species described here, namely L. brevispora sp. nov. on Pinus sp. from Mexico and L. guatemalensis sp. nov. on Pinus oocarpa from Guatemala. Epitypes were also designated for L. acicola and L. longispora to resolve the genetic application of these names. 
  L3  - 10.3767/003158512X661282
  JF  - Persoonia
  VL  - 29
  IS  - 
  SP  - 101
  EP  - 115
  ER  -