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Citation for Study 10593

Citation title: "Genetic variation and phylogeny of the cosmopolitan marine genus Tubificoides (Annelida: Clitellata: Naididae: Tubificinae)".

Study name: "Genetic variation and phylogeny of the cosmopolitan marine genus Tubificoides (Annelida: Clitellata: Naididae: Tubificinae)".

This study is part of submission 10583 (Status: Published).

Citation

Kvist S., Sarkar I.N., & Ers?us C. 2010. Genetic variation and phylogeny of the cosmopolitan marine genus Tubificoides (Annelida: Clitellata: Naididae: Tubificinae). Molecular Phylogenetics and Evolution, .

Authors

• Kvist S.
• Sarkar I.N.
• Ers?us C.

Abstract

Prior attempts to resolve the phylogenetic relationships of the cosmopolitan, marine clitellate genus Tubificoides, using only morphology, resulted in unresolved trees. In this study, three mitochondrial and three nuclear loci (5912 aligned sites) were analyzed, representing 14 morphologically separate species. Genetic distances within and between these forms on the basis of the mitochondrial genes (COI, 16S and 12S) revealed that 18 distinct mitochondrial lineages were represented in the data set. After analyzing also nuclear data (28S, 18S and ITS) we conclude that 17 separately evolving lineages (i.e., phylogenetic species) were represented, including three new, cryptic species closely related to T. pseudogaster, T. amplivasatus and T. insularis, respectively. Special emphasis was put on the DNA barcoding gene (COI), which was subject to haplotype diversity analysis and, for four species, diagnostic position (as determined by the Characteristic Attribute Organization System [CAOS]) screening. Typically, the intralineage variation was 1-2 orders of magnitude smaller than the interlineage divergence, making COI useful for identification of species within Tubificoides. Monophyly of the genus is supported and the evolutionary history of parts of the genus is revealed by phylogenetic analysis of the combined data set. A northern hemisphere origin of the genus is suggested, and most of the widely distributed species are members of one particular clade. Two morphological characters previously emphasized in Tubificoides taxonomy (hair chaetae and cuticular papillation) were optimized on the phylogenetic tree, revealing considerable homoplasy, belying the utility of these features as phylogenetic markers.

Keywords

Genetic variation; Haplotype diversity; Species delimitation; Invasive species; Phylogeny; Combined analysis; DNA barcoding; Tubificoides; Tubificinae; Naididae

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S10593
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref19047,
  	 author = {Sebastian  Kvist and Indra Neil Sarkar and C.  Ers?us},
  	 title = {Genetic variation and phylogeny of the cosmopolitan marine genus Tubificoides (Annelida: Clitellata: Naididae: Tubificinae)},
  	 year = {2010},
  	 keywords = {Genetic variation; Haplotype diversity; Species delimitation; Invasive species; Phylogeny; Combined analysis; DNA barcoding; Tubificoides; Tubificinae; Naididae},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Molecular Phylogenetics and Evolution},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Prior attempts to resolve the phylogenetic relationships of the cosmopolitan, marine clitellate genus Tubificoides, using only morphology, resulted in unresolved trees. In this study, three mitochondrial and three nuclear loci (5912 aligned sites) were analyzed, representing 14 morphologically separate species. Genetic distances within and between these forms on the basis of the mitochondrial genes (COI, 16S and 12S) revealed that 18 distinct mitochondrial lineages were represented in the data set. After analyzing also nuclear data (28S, 18S and ITS) we conclude that 17 separately evolving lineages (i.e., phylogenetic species) were represented, including three new, cryptic species closely related to T. pseudogaster, T. amplivasatus and T. insularis, respectively. Special emphasis was put on the DNA barcoding gene (COI), which was subject to haplotype diversity analysis and, for four species, diagnostic position (as determined by the Characteristic Attribute Organization System [CAOS]) screening. Typically, the intralineage variation was 1-2 orders of magnitude smaller than the interlineage divergence, making COI useful for identification of species within Tubificoides. Monophyly of the genus is supported and the evolutionary history of parts of the genus is revealed by phylogenetic analysis of the combined data set. A northern hemisphere origin of the genus is suggested, and most of the widely distributed species are members of one particular clade. Two morphological characters previously emphasized in Tubificoides taxonomy (hair chaetae and cuticular papillation) were optimized on the phylogenetic tree, revealing considerable homoplasy, belying the utility of these features as phylogenetic markers.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 19047
  AU  - Kvist,Sebastian 
  AU  - Sarkar,Indra Neil
  AU  - Ers?us,C. 
  T1  - Genetic variation and phylogeny of the cosmopolitan marine genus Tubificoides (Annelida: Clitellata: Naididae: Tubificinae)
  PY  - 2010
  KW  - Genetic variation; Haplotype diversity; Species delimitation; Invasive species; Phylogeny; Combined analysis; DNA barcoding; Tubificoides; Tubificinae; Naididae
  UR  - http://dx.doi.org/
  N2  - Prior attempts to resolve the phylogenetic relationships of the cosmopolitan, marine clitellate genus Tubificoides, using only morphology, resulted in unresolved trees. In this study, three mitochondrial and three nuclear loci (5912 aligned sites) were analyzed, representing 14 morphologically separate species. Genetic distances within and between these forms on the basis of the mitochondrial genes (COI, 16S and 12S) revealed that 18 distinct mitochondrial lineages were represented in the data set. After analyzing also nuclear data (28S, 18S and ITS) we conclude that 17 separately evolving lineages (i.e., phylogenetic species) were represented, including three new, cryptic species closely related to T. pseudogaster, T. amplivasatus and T. insularis, respectively. Special emphasis was put on the DNA barcoding gene (COI), which was subject to haplotype diversity analysis and, for four species, diagnostic position (as determined by the Characteristic Attribute Organization System [CAOS]) screening. Typically, the intralineage variation was 1-2 orders of magnitude smaller than the interlineage divergence, making COI useful for identification of species within Tubificoides. Monophyly of the genus is supported and the evolutionary history of parts of the genus is revealed by phylogenetic analysis of the combined data set. A northern hemisphere origin of the genus is suggested, and most of the widely distributed species are members of one particular clade. Two morphological characters previously emphasized in Tubificoides taxonomy (hair chaetae and cuticular papillation) were optimized on the phylogenetic tree, revealing considerable homoplasy, belying the utility of these features as phylogenetic markers.
  L3  - 
  JF  - Molecular Phylogenetics and Evolution
  VL  - 
  IS  - 
  ER  -