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Citation for Study 10778

Citation title: "Nuclear and chloroplast DNA suggest a complex single origin for the threatened allopolyploid Solidago houghtonii (Asteraceae) involving reticulate evolution and introgression".

Study name: "Nuclear and chloroplast DNA suggest a complex single origin for the threatened allopolyploid Solidago houghtonii (Asteraceae) involving reticulate evolution and introgression".

This study is part of submission 10768 (Status: Published).

Citation

Laureto P., & Barkman T. 2010. Nuclear and chloroplast DNA suggest a complex single origin for the threatened allopolyploid Solidago houghtonii (Asteraceae) involving reticulate evolution and introgression. Systematic Botany, .

Authors

• Laureto P.
• Barkman T. (submitter) 269-387-2776

Abstract

Abstract?Solidago houghtonii is a federally threatened polyploid plant species likely of hybrid origin. Several hypothesized combinations of parental species have been suggested but none have been phylogenetically tested. Additionally, it is unclear whether the species resulted from a single or polytopic origin. To study the evolutionary history of S. houghtonii we sequenced four noncoding cpDNA loci (accD-psaI, psbA-trnH, trnL-trnF, rps16-trnQ), and the nrDNA ITS and 3'ETS regions for four accessions of S. houghtonii, which span its geographic range, and 25 other species of Solidago including all sympatric species. Polymorphisms within the direct nrDNA sequences of all S. houghtonii accessions indicated the presence of multiple homeologue types. These were separated by molecular cloning of the 3055 bp 3'ETS ? ITS region, allowing us to positively link the ETS and ITS homeologue types. Phylogenetic analyses of the nuclear and chloroplast datasets revealed incongruent topologies. Analysis of cloned nrDNA sequence data indicated that S. riddellii, S. ptarmicoides, and S. ohioensis have contributed to the nuclear genome of S. houghtonii. Analysis of cpDNA sequence data revealed the presence of multiple insertions/deletions that are shared by all accessions. The unique pattern of indels was also recovered in S. gigantea. Phylogenetic analysis of the cpDNA sequence data and coded indels indicate S. gigantea is the maternal genome donor. However, we did not recover a S. gigantea nrDNA sequence type. Taken together, these data reveal both a single origin and a complex pattern of reticulation that is consistent not only with the hypothesized allohexaploid nature of this species, but also with chloroplast capture of cpDNA from an unexpected source through introgression.

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S10778
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref19140,
  	 author = {Pamela  Laureto and Todd  Barkman},
  	 title = {Nuclear and chloroplast DNA suggest a complex single origin for the threatened allopolyploid Solidago houghtonii (Asteraceae) involving reticulate evolution and introgression},
  	 year = {2010},
  	 keywords = {},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Systematic Botany},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Abstract?Solidago houghtonii is a federally threatened polyploid plant species likely of hybrid origin. Several hypothesized combinations of parental species have been suggested but none have been phylogenetically tested.  Additionally, it is unclear whether the species resulted from a single or polytopic origin.  To study the evolutionary history of S. houghtonii we sequenced four noncoding cpDNA loci (accD-psaI, psbA-trnH, trnL-trnF, rps16-trnQ), and the nrDNA ITS and 3'ETS regions for four accessions of S. houghtonii, which span its geographic range, and 25 other species of Solidago including all sympatric species.  Polymorphisms within the direct nrDNA sequences of all S. houghtonii accessions indicated the presence of multiple homeologue types.  These were separated by molecular cloning of the 3055 bp 3'ETS ? ITS region, allowing us to positively link the ETS and ITS homeologue types.  Phylogenetic analyses of the nuclear and chloroplast datasets revealed incongruent topologies.  Analysis of cloned nrDNA sequence data indicated that S. riddellii, S. ptarmicoides, and S. ohioensis have contributed to the nuclear genome of S. houghtonii.  Analysis of cpDNA sequence data revealed the presence of multiple insertions/deletions that are shared by all accessions. The unique pattern of indels was also recovered in S. gigantea.  Phylogenetic analysis of the cpDNA sequence data and coded indels indicate S. gigantea is the maternal genome donor.  However, we did not recover a S. gigantea nrDNA sequence type.  Taken together, these data reveal both a single origin and a complex pattern of reticulation that is consistent not only with the hypothesized allohexaploid nature of this species, but also with chloroplast capture of cpDNA from an unexpected source through introgression.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 19140
  AU  - Laureto,Pamela 
  AU  - Barkman,Todd 
  T1  - Nuclear and chloroplast DNA suggest a complex single origin for the threatened allopolyploid Solidago houghtonii (Asteraceae) involving reticulate evolution and introgression
  PY  - 2010
  KW  - 
  UR  - http://dx.doi.org/
  N2  - Abstract?Solidago houghtonii is a federally threatened polyploid plant species likely of hybrid origin. Several hypothesized combinations of parental species have been suggested but none have been phylogenetically tested.  Additionally, it is unclear whether the species resulted from a single or polytopic origin.  To study the evolutionary history of S. houghtonii we sequenced four noncoding cpDNA loci (accD-psaI, psbA-trnH, trnL-trnF, rps16-trnQ), and the nrDNA ITS and 3'ETS regions for four accessions of S. houghtonii, which span its geographic range, and 25 other species of Solidago including all sympatric species.  Polymorphisms within the direct nrDNA sequences of all S. houghtonii accessions indicated the presence of multiple homeologue types.  These were separated by molecular cloning of the 3055 bp 3'ETS ? ITS region, allowing us to positively link the ETS and ITS homeologue types.  Phylogenetic analyses of the nuclear and chloroplast datasets revealed incongruent topologies.  Analysis of cloned nrDNA sequence data indicated that S. riddellii, S. ptarmicoides, and S. ohioensis have contributed to the nuclear genome of S. houghtonii.  Analysis of cpDNA sequence data revealed the presence of multiple insertions/deletions that are shared by all accessions. The unique pattern of indels was also recovered in S. gigantea.  Phylogenetic analysis of the cpDNA sequence data and coded indels indicate S. gigantea is the maternal genome donor.  However, we did not recover a S. gigantea nrDNA sequence type.  Taken together, these data reveal both a single origin and a complex pattern of reticulation that is consistent not only with the hypothesized allohexaploid nature of this species, but also with chloroplast capture of cpDNA from an unexpected source through introgression.
  L3  - 
  JF  - Systematic Botany
  VL  - 
  IS  - 
  ER  -