@ARTICLE{TreeBASE2Ref23357,
author = {Eliana Borges Rivas and Estev?o Cardoso de Almeida B?di and Ricardo Harakava and Fabio Gregori and Marcos Cesar Goncalves},
title = {Phylogeny and recombination analysis of Lily symptomless virus and Lily mottle virus from Brazil },
year = {2014},
keywords = {Carlavirus, Potyvirus, Multiplex PCR},
doi = {},
url = {http://},
pmid = {},
journal = {Plant Disease},
volume = {},
number = {},
pages = {},
abstract = {Lilies are cultivated in Brazil from imported bulbs that might be infected by exotic or quarantine viruses, such as Lily mottle virus (LMoV) and Lily symptomless virus (LSV).
Since LMoV and LSV have been recently reported in Brazil, we detected the spreading of these viruses in 12 cultivation areas using simplex and multiplex RT-PCR with specific
primers designed for the respective coat protein (CP) genes amplification. Eight fragments with 800 nt length obtained from the LMoV-infected and nine with 600 nt from LSV-
infected lilies were sequenced. Phylogenetic analysis using the CP sequences clustered two Brazilian LMoV isolates in the same subtree with Tulip breaking virus-lily strain (sin.
LMoV) and Tulip band breaking virus, both from Japan. The other six LMoV Brazilian isolates share a common ancestor with isolates from different countries. LSV isolates from
Brazil clustered with isolates from Asia and The Netherlands, sharing a common ancestor.Recombination detection analysis with partial CP sequences from both viruses revealed a
LMoV recombinant isolate (S44147-Netherlands) whereas none event was supported for recombination among LSV isolates. To our knowledge, this is the ?rst report on evidence
of recombination between LMoV isolates and of a proposal for its classification into groups.}
}
Citation for Study 16007
Citation title:
"Phylogeny and recombination analysis of Lily symptomless virus and Lily mottle virus from Brazil ".
Study name:
"Phylogeny and recombination analysis of Lily symptomless virus and Lily mottle virus from Brazil ".
This study is part of submission 16007
(Status: Published).
Citation
Rivas E.B., B?di E.C., Harakava R., Gregori F., & Goncalves M.C. 2014. Phylogeny and recombination analysis of Lily symptomless virus and Lily mottle virus from Brazil. Plant Disease, .
Authors
-
Rivas E.B.
55-11-50871722
-
B?di E.C.
-
Harakava R.
55-11-55490114
-
Gregori F.
(submitter)
55 11 30911386
-
Goncalves M.C.
Abstract
Lilies are cultivated in Brazil from imported bulbs that might be infected by exotic or quarantine viruses, such as Lily mottle virus (LMoV) and Lily symptomless virus (LSV).
Since LMoV and LSV have been recently reported in Brazil, we detected the spreading of these viruses in 12 cultivation areas using simplex and multiplex RT-PCR with specific
primers designed for the respective coat protein (CP) genes amplification. Eight fragments with 800 nt length obtained from the LMoV-infected and nine with 600 nt from LSV-
infected lilies were sequenced. Phylogenetic analysis using the CP sequences clustered two Brazilian LMoV isolates in the same subtree with Tulip breaking virus-lily strain (sin.
LMoV) and Tulip band breaking virus, both from Japan. The other six LMoV Brazilian isolates share a common ancestor with isolates from different countries. LSV isolates from
Brazil clustered with isolates from Asia and The Netherlands, sharing a common ancestor.Recombination detection analysis with partial CP sequences from both viruses revealed a
LMoV recombinant isolate (S44147-Netherlands) whereas none event was supported for recombination among LSV isolates. To our knowledge, this is the ?rst report on evidence
of recombination between LMoV isolates and of a proposal for its classification into groups.
Keywords
Carlavirus, Potyvirus, Multiplex PCR
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S16007
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref23357,
author = {Eliana Borges Rivas and Estev?o Cardoso de Almeida B?di and Ricardo Harakava and Fabio Gregori and Marcos Cesar Goncalves},
title = {Phylogeny and recombination analysis of Lily symptomless virus and Lily mottle virus from Brazil },
year = {2014},
keywords = {Carlavirus, Potyvirus, Multiplex PCR},
doi = {},
url = {http://},
pmid = {},
journal = {Plant Disease},
volume = {},
number = {},
pages = {},
abstract = {Lilies are cultivated in Brazil from imported bulbs that might be infected by exotic or quarantine viruses, such as Lily mottle virus (LMoV) and Lily symptomless virus (LSV).
Since LMoV and LSV have been recently reported in Brazil, we detected the spreading of these viruses in 12 cultivation areas using simplex and multiplex RT-PCR with specific
primers designed for the respective coat protein (CP) genes amplification. Eight fragments with 800 nt length obtained from the LMoV-infected and nine with 600 nt from LSV-
infected lilies were sequenced. Phylogenetic analysis using the CP sequences clustered two Brazilian LMoV isolates in the same subtree with Tulip breaking virus-lily strain (sin.
LMoV) and Tulip band breaking virus, both from Japan. The other six LMoV Brazilian isolates share a common ancestor with isolates from different countries. LSV isolates from
Brazil clustered with isolates from Asia and The Netherlands, sharing a common ancestor.Recombination detection analysis with partial CP sequences from both viruses revealed a
LMoV recombinant isolate (S44147-Netherlands) whereas none event was supported for recombination among LSV isolates. To our knowledge, this is the ?rst report on evidence
of recombination between LMoV isolates and of a proposal for its classification into groups.}
}
- Show RIS reference
TY - JOUR
ID - 23357
AU - Rivas,Eliana Borges
AU - B?di,Estev?o Cardoso de Almeida
AU - Harakava,Ricardo
AU - Gregori,Fabio
AU - Goncalves,Marcos Cesar
T1 - Phylogeny and recombination analysis of Lily symptomless virus and Lily mottle virus from Brazil
PY - 2014
KW - Carlavirus
KW - Potyvirus
KW - Multiplex PCR
UR - http://dx.doi.org/
N2 - Lilies are cultivated in Brazil from imported bulbs that might be infected by exotic or quarantine viruses, such as Lily mottle virus (LMoV) and Lily symptomless virus (LSV).
Since LMoV and LSV have been recently reported in Brazil, we detected the spreading of these viruses in 12 cultivation areas using simplex and multiplex RT-PCR with specific
primers designed for the respective coat protein (CP) genes amplification. Eight fragments with 800 nt length obtained from the LMoV-infected and nine with 600 nt from LSV-
infected lilies were sequenced. Phylogenetic analysis using the CP sequences clustered two Brazilian LMoV isolates in the same subtree with Tulip breaking virus-lily strain (sin.
LMoV) and Tulip band breaking virus, both from Japan. The other six LMoV Brazilian isolates share a common ancestor with isolates from different countries. LSV isolates from
Brazil clustered with isolates from Asia and The Netherlands, sharing a common ancestor.Recombination detection analysis with partial CP sequences from both viruses revealed a
LMoV recombinant isolate (S44147-Netherlands) whereas none event was supported for recombination among LSV isolates. To our knowledge, this is the ?rst report on evidence
of recombination between LMoV isolates and of a proposal for its classification into groups.
L3 -
JF - Plant Disease
VL -
IS -
ER -