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Citation for Study 21131

Citation title: "Population structure and biodiversity of Pectobacterium parmentieri isolated from potato fields in temperate climate".

Study name: "Population structure and biodiversity of Pectobacterium parmentieri isolated from potato fields in temperate climate".

This study is part of submission 21131 (Status: Published).

Citation

Zoledowska S., Motyka A., Zukowska D., Sledz W., & Lojkowska E. 2017. Population structure and biodiversity of Pectobacterium parmentieri isolated from potato fields in temperate climate. Plant Disease, .

Authors

• Zoledowska S.
• Motyka A.
• Zukowska D.
• Sledz W.
• Lojkowska E.

Abstract

Pectobacterium parmentieri (formerly Pectobacterium wasabiae) is a newly established species of pectinolytic plant pathogenic bacteria responsible for the symptoms of soft rot and blackleg on potato. In this work, we describe biodiversity and the population structure of P. parmentieri strains isolated during two consecutive growing seasons from the seed potato fields in Poland. Altogether 441 samples of diseased potato tubers, potato plants or accompanying weeds were collected throughout the country and tested for the presence of P. parmentieri by molecular identification methods. We noted that P. parmentieri strains commonly occurr in almost all regions of Poland. Besides, these isolates constituted significant fraction of pectinolytic bacteria from seed potato fields as 16% (2013) and 13% (2014) of the analyzed plant samples were infected with P. parmentieri. Subsequently, a detailed characterization of the obtained strains was conducted basing on repetitive sequences profiling, recA-gene based phylogeny and phenotypic features. By applying REP-PCR, we revealed the presence of five distinct genomic profiles among P. parmentieri strains with profile I being the most abundant (approx. 44%). The performed recA gene-based phylogenetic analysis divided P. parmentieri isolates into two distinct clades, although the strains orginating from different years did not group separetly. Evaluation of the phenotypic traits playing crucial roles in the virulence of pectinolytic bacteria, namely: pectinase, cellulase and protease activities, siderophore production in addition to potato tissue maceration, swimming and swarming motility indicated some differnces among the characterized strains. To the best of our knowledge, this is the first study that describes biodiversity and the population structure of P. parmentieri isolated in two growing seasons under temperate climate conditions and hence illustrates high heterogenicity within this species.

Keywords

blackleg, pectinolytic erwinias, Pectobacterium wasabiae, recA gene, soft rot.

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S21131
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref27309,
  	 author = {Sabina  Zoledowska and Agata  Motyka and Dominika  Zukowska and Wojciech  Sledz and Ewa  Lojkowska},
  	 title = {Population structure and biodiversity of Pectobacterium parmentieri isolated from potato fields in temperate climate},
  	 year = {2017},
  	 keywords = {blackleg, pectinolytic erwinias, Pectobacterium wasabiae, recA gene, soft rot. },
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Plant Disease},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Pectobacterium parmentieri (formerly Pectobacterium wasabiae) is a newly established species of pectinolytic plant pathogenic bacteria responsible for the symptoms of soft rot and blackleg on potato. In this work, we describe biodiversity and the population structure of P. parmentieri strains isolated during two consecutive growing seasons from the seed potato fields in Poland. Altogether 441 samples of diseased potato tubers, potato plants or accompanying weeds were collected throughout the country and tested for the presence of P. parmentieri by molecular identification methods. We noted that P. parmentieri strains commonly occurr in almost all regions of Poland. Besides, these isolates constituted significant fraction of pectinolytic bacteria from seed potato fields as 16% (2013) and 13% (2014) of the analyzed plant samples were infected with P. parmentieri. Subsequently, a detailed characterization of the obtained strains was conducted basing on repetitive sequences profiling, recA-gene based phylogeny and phenotypic features. By applying REP-PCR, we revealed the presence of five distinct genomic profiles among P. parmentieri strains with profile I being the most abundant (approx. 44%). The performed recA gene-based phylogenetic analysis divided P. parmentieri isolates into two distinct clades, although the strains orginating from different years did not group separetly. Evaluation of the phenotypic traits playing crucial roles in the virulence of pectinolytic bacteria, namely: pectinase, cellulase and protease activities, siderophore production in addition to potato tissue maceration, swimming and swarming motility indicated some differnces among the characterized strains. To the best of our knowledge, this is the first study that describes biodiversity and the population structure of P. parmentieri isolated in two growing seasons under temperate climate conditions and hence illustrates high heterogenicity within this species.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 27309
  AU  - Zoledowska,Sabina 
  AU  - Motyka,Agata 
  AU  - Zukowska,Dominika 
  AU  - Sledz,Wojciech 
  AU  - Lojkowska,Ewa 
  T1  - Population structure and biodiversity of Pectobacterium parmentieri isolated from potato fields in temperate climate
  PY  - 2017
  KW  - blackleg
  KW  - pectinolytic erwinias
  KW  - Pectobacterium wasabiae
  KW  - recA gene
  KW  - soft rot. 
  UR  - http://dx.doi.org/
  N2  - Pectobacterium parmentieri (formerly Pectobacterium wasabiae) is a newly established species of pectinolytic plant pathogenic bacteria responsible for the symptoms of soft rot and blackleg on potato. In this work, we describe biodiversity and the population structure of P. parmentieri strains isolated during two consecutive growing seasons from the seed potato fields in Poland. Altogether 441 samples of diseased potato tubers, potato plants or accompanying weeds were collected throughout the country and tested for the presence of P. parmentieri by molecular identification methods. We noted that P. parmentieri strains commonly occurr in almost all regions of Poland. Besides, these isolates constituted significant fraction of pectinolytic bacteria from seed potato fields as 16% (2013) and 13% (2014) of the analyzed plant samples were infected with P. parmentieri. Subsequently, a detailed characterization of the obtained strains was conducted basing on repetitive sequences profiling, recA-gene based phylogeny and phenotypic features. By applying REP-PCR, we revealed the presence of five distinct genomic profiles among P. parmentieri strains with profile I being the most abundant (approx. 44%). The performed recA gene-based phylogenetic analysis divided P. parmentieri isolates into two distinct clades, although the strains orginating from different years did not group separetly. Evaluation of the phenotypic traits playing crucial roles in the virulence of pectinolytic bacteria, namely: pectinase, cellulase and protease activities, siderophore production in addition to potato tissue maceration, swimming and swarming motility indicated some differnces among the characterized strains. To the best of our knowledge, this is the first study that describes biodiversity and the population structure of P. parmentieri isolated in two growing seasons under temperate climate conditions and hence illustrates high heterogenicity within this species.
  L3  - 
  JF  - Plant Disease
  VL  - 
  IS  - 
  ER  -