CiteULike

Delicious

Connotea

Citation for Study 23281

Citation title: "Trichothecene Production and Pathogenicity of Fusaria Isolated from Symptomatic Soybean Roots in Ethiopia and Ghana".

Study name: "Trichothecene Production and Pathogenicity of Fusaria Isolated from Symptomatic Soybean Roots in Ethiopia and Ghana".

This study is part of submission 23281 (Status: Published).

Citation

Hartman G.L., Mccormick S.P., & O'donnell K. 2018. Trichothecene Production and Pathogenicity of Fusaria Isolated from Symptomatic Soybean Roots in Ethiopia and Ghana. Plant Disease, .

Authors

• Hartman G.L.
• Mccormick S.P.
• O'donnell K. 309-681-6383

Abstract

Numerous pathogen surveys have revealed diverse Fusarium species threaten soybean production in North and South America. However, little research has been conducted to characterize fusarial pathogens of this crop in Sub-Saharan Africa. Therefore, in this study we isolated Fusarium species from root segments of symptomatic soybeans grown in Ethiopia and Ghana. Isolates were identified using DNA sequence data, assayed for trichothecene mycotoxin production in vitro and tested for pathogenicity on soybean. Molecular phylogenetic analyses revealed the trichothecene mycotoxin-producing isolates comprised three undescribed species within the F. incarnatum-equiseti (FIESC) and F. sambucinum species complexes (FSAMSC). Mycotoxin analyses demonstrated that 18 of the 21 isolates tested possessed the ability to produce the type B trichothecene 4,15-diacetylnivalenol (4,15-diANIV) or T-2 toxin and related type A neosolaniol trichothecenes when grown in agmatine broth or on a solid rice culture. Of the 13 isolates tested for their impact on seed germination, five comprising three undescribed phylospecies (i.e., Fusarium sp. #3, Fusarium sp. FIESC 2 and Fusarium sp. FIESC 29) completely inhibited germination, whereas four caused no reduction in germination. Root lesions induced by all 13 isolates were greater than the uninoculated negative control. Additional variation among the isolates was reflected in differences ( = 0.05) in percentage of lesion lengths per total root length, which ranged from 34 to 67%. This is the first report characterizing the genetic diversity and mycotoxigenic potential of fusaria isolated from soybean roots in Ghana and Ethiopia.

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S23281
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref28819,
  	 author = {Glen L Hartman and Susan P. McCormick and Kerry  O'Donnell},
  	 title = {Trichothecene Production and Pathogenicity of Fusaria Isolated from Symptomatic Soybean Roots in Ethiopia and Ghana},
  	 year = {2018},
  	 keywords = {},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Plant Disease},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Numerous pathogen surveys have revealed diverse Fusarium species threaten soybean production in North and South America.  However, little research has been conducted to characterize fusarial pathogens of this crop in Sub-Saharan Africa.  Therefore, in this study we isolated Fusarium species from root segments of symptomatic soybeans grown in Ethiopia and Ghana.  Isolates were identified using DNA sequence data, assayed for trichothecene mycotoxin production in vitro and tested for pathogenicity on soybean.  Molecular phylogenetic analyses revealed the trichothecene mycotoxin-producing isolates comprised three undescribed species within the F. incarnatum-equiseti (FIESC) and F. sambucinum species complexes (FSAMSC).  Mycotoxin analyses demonstrated that 18 of the 21 isolates tested possessed the ability to produce the type B trichothecene 4,15-diacetylnivalenol (4,15-diANIV) or T-2 toxin and related type A neosolaniol trichothecenes when grown in agmatine broth or on a solid rice culture.  Of the 13 isolates tested for their impact on seed germination, five comprising three undescribed phylospecies (i.e., Fusarium sp. #3, Fusarium sp. FIESC 2 and Fusarium sp. FIESC 29) completely inhibited germination, whereas four caused no reduction in germination. Root lesions induced by all 13 isolates were greater than the uninoculated negative control.  Additional variation among the isolates was reflected in differences ( = 0.05) in percentage of lesion lengths per total root length, which ranged from 34 to 67%.  This is the first report characterizing the genetic diversity and mycotoxigenic potential of fusaria isolated from soybean roots in Ghana and Ethiopia.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 28819
  AU  - Hartman,Glen L
  AU  - McCormick,Susan P.
  AU  - O'Donnell,Kerry 
  T1  - Trichothecene Production and Pathogenicity of Fusaria Isolated from Symptomatic Soybean Roots in Ethiopia and Ghana
  PY  - 2018
  KW  - 
  UR  - http://dx.doi.org/
  N2  - Numerous pathogen surveys have revealed diverse Fusarium species threaten soybean production in North and South America.  However, little research has been conducted to characterize fusarial pathogens of this crop in Sub-Saharan Africa.  Therefore, in this study we isolated Fusarium species from root segments of symptomatic soybeans grown in Ethiopia and Ghana.  Isolates were identified using DNA sequence data, assayed for trichothecene mycotoxin production in vitro and tested for pathogenicity on soybean.  Molecular phylogenetic analyses revealed the trichothecene mycotoxin-producing isolates comprised three undescribed species within the F. incarnatum-equiseti (FIESC) and F. sambucinum species complexes (FSAMSC).  Mycotoxin analyses demonstrated that 18 of the 21 isolates tested possessed the ability to produce the type B trichothecene 4,15-diacetylnivalenol (4,15-diANIV) or T-2 toxin and related type A neosolaniol trichothecenes when grown in agmatine broth or on a solid rice culture.  Of the 13 isolates tested for their impact on seed germination, five comprising three undescribed phylospecies (i.e., Fusarium sp. #3, Fusarium sp. FIESC 2 and Fusarium sp. FIESC 29) completely inhibited germination, whereas four caused no reduction in germination. Root lesions induced by all 13 isolates were greater than the uninoculated negative control.  Additional variation among the isolates was reflected in differences ( = 0.05) in percentage of lesion lengths per total root length, which ranged from 34 to 67%.  This is the first report characterizing the genetic diversity and mycotoxigenic potential of fusaria isolated from soybean roots in Ghana and Ethiopia.
  L3  - 
  JF  - Plant Disease
  VL  - 
  IS  - 
  ER  -