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Citation for Study 1401

Citation title: "Phylogenetic relationships between members of the crucifer pathogenic Leptosphaeria maculans species complex as shown by mating type (MAT1-2), actin and beta tubulin sequences".

This study was previously identified under the legacy study ID S1334 (Status: Published).

Citation

Voigt K., Cozijnsen A., Kroymann J., P?ggeler S., & Howlett B. 2005. Phylogenetic relationships between members of the crucifer pathogenic Leptosphaeria maculans species complex as shown by mating type (MAT1-2), actin and beta tubulin sequences. Molecular Phylogenetics and Evolution, 37(2): 541-557.

Authors

• Voigt K.
• Cozijnsen A.
• Kroymann J.
• P?ggeler S.
• Howlett B.

Abstract

The dothideomycetous fungus Leptosphaeria maculans comprises a complex of species differing in specificity and pathogenicity on Brassica napus. Twenty eight isolates were investigated and compared to 20 other species of the Pleosporales order. Sequences of the mating type MAT1-2 (23), fragments of actin (48) and beta tubulin (45) genes were determined and used for phylogenetic analyses inferred by maximum parsimony, distance, maximum likelihood and Bayesian approaches. These different approaches using single genes essentially confirmed findings using concatenated sequences. L. maculans formed a monophyletic group separate from L. biglobosa. The L. biglobosa clade encompasses five subclades. This is generally consistent with a classification made previously on the basis of internal transcribed sequences of the ribosomal DNA repeat. The propensity for purifying and neutral evolution of the three genes was determined using sliding window analysis, a technique not previously applied to genes of filamentous fungi. For members of the L. maculans species complex, this approach showed that in comparison to actin and beta tubulin, exonic sequences of MAT1-2 were more diverse and appeared to evolve at a faster rate. However, different regions of MAT1-2 displayed different degrees of sequence conservation. The more conserved upstream region (including the High Mobility Group domain) may be better suited for interspecies differentiation, while the more diverse downstream region is more appropriate for intraspecies comparisons.

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S1401
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref17990,
  	 author = {Kerstin  Voigt and Anton J.  Cozijnsen and J?rgen  Kroymann and Stefanie  P?ggeler and Barbara J.  Howlett},
  	 title = {Phylogenetic relationships between members of the crucifer pathogenic Leptosphaeria maculans species complex as shown by mating type (MAT1-2), actin and beta tubulin sequences},
  	 year = {2005},
  	 keywords = {},
  	 doi = {},
  	 url = {},
  	 pmid = {},
  	 journal = {Molecular Phylogenetics and Evolution},
  	 volume = {37},
  	 number = {2},
  	 pages = {541--557},
  	 abstract = {The dothideomycetous fungus Leptosphaeria maculans comprises a complex of species differing in specificity and pathogenicity on Brassica napus. Twenty eight isolates were investigated and compared to 20 other species of the Pleosporales order. Sequences of the mating type MAT1-2 (23), fragments of actin (48) and beta tubulin (45) genes were determined and used for phylogenetic analyses inferred by maximum parsimony, distance, maximum likelihood and Bayesian approaches. These different approaches using single genes essentially confirmed findings using concatenated sequences. L. maculans formed a monophyletic group separate from L. biglobosa. The L. biglobosa clade encompasses five subclades. This is generally consistent with a classification made previously on the basis of internal transcribed sequences of the ribosomal DNA repeat. The propensity for purifying and neutral evolution of the three genes was determined using sliding window analysis, a technique not previously applied to genes of filamentous fungi. For members of the L. maculans species complex, this approach showed that in comparison to actin and beta tubulin, exonic sequences of MAT1-2 were more diverse and appeared to evolve at a faster rate. However, different regions of MAT1-2 displayed different degrees of sequence conservation. The more conserved upstream region (including the High Mobility Group domain) may be better suited for interspecies differentiation, while the more diverse downstream region is more appropriate for intraspecies comparisons.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 17990
  AU  - Voigt,Kerstin 
  AU  - Cozijnsen,Anton J. 
  AU  - Kroymann,J?rgen 
  AU  - P?ggeler,Stefanie 
  AU  - Howlett,Barbara J. 
  T1  - Phylogenetic relationships between members of the crucifer pathogenic Leptosphaeria maculans species complex as shown by mating type (MAT1-2), actin and beta tubulin sequences
  PY  - 2005
  UR  - 
  N2  - The dothideomycetous fungus Leptosphaeria maculans comprises a complex of species differing in specificity and pathogenicity on Brassica napus. Twenty eight isolates were investigated and compared to 20 other species of the Pleosporales order. Sequences of the mating type MAT1-2 (23), fragments of actin (48) and beta tubulin (45) genes were determined and used for phylogenetic analyses inferred by maximum parsimony, distance, maximum likelihood and Bayesian approaches. These different approaches using single genes essentially confirmed findings using concatenated sequences. L. maculans formed a monophyletic group separate from L. biglobosa. The L. biglobosa clade encompasses five subclades. This is generally consistent with a classification made previously on the basis of internal transcribed sequences of the ribosomal DNA repeat. The propensity for purifying and neutral evolution of the three genes was determined using sliding window analysis, a technique not previously applied to genes of filamentous fungi. For members of the L. maculans species complex, this approach showed that in comparison to actin and beta tubulin, exonic sequences of MAT1-2 were more diverse and appeared to evolve at a faster rate. However, different regions of MAT1-2 displayed different degrees of sequence conservation. The more conserved upstream region (including the High Mobility Group domain) may be better suited for interspecies differentiation, while the more diverse downstream region is more appropriate for intraspecies comparisons.
  L3  - 
  JF  - Molecular Phylogenetics and Evolution
  VL  - 37
  IS  - 2
  SP  - 541
  EP  - 557
  ER  -