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Citation for Study 23504

Citation title: "Genetic diversity and vegetative compatibility of?Fusarium solani species complex of strawberry in Spain".

Study name: "Genetic diversity and vegetative compatibility of?Fusarium solani species complex of strawberry in Spain".

This study is part of submission 23504 (Status: Published).

Citation

De la lastra E., Villarino M., Astacio J., Larena I., De cal A., & Capote N. 2018. Genetic diversity and vegetative compatibility of?Fusarium solani species complex of strawberry in Spain. Phytopathology, .

Authors

• De la lastra E. (submitter)
• Villarino M.
• Astacio J.
• Larena I.
• De cal A.
• Capote N.

Abstract

Fusarium solani is a soil-borne fungus that is pathogen on more than one hundred plants. It is the causal agent of crown and root rot in strawberry. We collected 101 F. solani isolates from diseased plants and soils from two distinct geographic areas of strawberry production in Spain: plant nurseries located in the North-Center of the country, and fruit production fields located in the South-West. Multilocus DNA sequences were constructed for the molecular identification of the isolates via Fusarium MLST online database, and to assess the phylogenetic relationships and diversity of the populations. In addition, the vegetative compatibility between isolates was analyzed based on complementation tests involving nitrate non-utilizing (nit) mutants. Phylogenetic analysis revealed that all strawberry-associated isolates were restricted to Clade 3 of FSSC. Forty-nine isolates from nursery population comprised of 4 distinct phylogenetic species i.e., FSSC 2 (F. keratoplasticum), FSSC 5 (F. solani sensu stricto), FSSC 9, and an unknown FSSC species, that matched with 5 vegetative compatibility groups (VCGs). Fifty-two isolates from fruit production fields comprised 5 strongly supported phylogenetic species i.e., FSSC 2, FSSC 3+4 (F. falciforme), FSSC 5, FSSC 6 (F. metavorans sp. nov.), and FSSC 11, distributed into 31 VCGs. Genetic and VCG variability of fruit production fields population was higher than those from nurseries. Genetic diversity analyses suggested that nurseries and fruit production fields are two different populations. The fact that some phylogenetic groups shared isolates from both populations, that heterocarion formation was detected between isolates from both populations, and that isolates from soils and diseased plants shared the same haplotype, suggests that infected runner plants and soils could be potential sources of inoculum of FSSC in fruit production fields.

Keywords

FSSC, Fusarium MLST, phylogenetic analysis, VCG, nursery, fruit production fields

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S23504
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref28966,
  	 author = {Eduardo  de la Lastra and Maria  Villarino and Juan Diego  Astacio and Inmaculada  Larena and Antonieta  De Cal and Nieves  Capote},
  	 title = {Genetic diversity and vegetative compatibility of?Fusarium solani species complex of strawberry in Spain},
  	 year = {2018},
  	 keywords = {FSSC, Fusarium MLST, phylogenetic analysis, VCG, nursery, fruit production fields},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Phytopathology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Fusarium solani is a soil-borne fungus that is pathogen on more than one hundred plants. It is the causal agent of crown and root rot in strawberry. We collected 101 F. solani isolates from diseased plants and soils from two distinct geographic areas of strawberry production in Spain: plant nurseries located in the North-Center of the country, and fruit production fields located in the South-West. Multilocus DNA sequences were constructed for the molecular identification of the isolates via Fusarium MLST online database, and to assess the phylogenetic relationships and diversity of the populations. In addition, the vegetative compatibility between isolates was analyzed based on complementation tests involving nitrate non-utilizing (nit) mutants. Phylogenetic analysis revealed that all strawberry-associated isolates were restricted to Clade 3 of FSSC. Forty-nine isolates from nursery population comprised of 4 distinct phylogenetic species i.e., FSSC 2 (F. keratoplasticum), FSSC 5 (F. solani sensu stricto), FSSC 9, and an unknown FSSC species, that matched with 5 vegetative compatibility groups (VCGs). Fifty-two isolates from fruit production fields comprised 5 strongly supported phylogenetic species i.e., FSSC 2, FSSC 3+4 (F. falciforme), FSSC 5, FSSC 6 (F. metavorans sp. nov.), and FSSC 11, distributed into 31 VCGs. Genetic and VCG variability of fruit production fields population was higher than those from nurseries. Genetic diversity analyses suggested that nurseries and fruit production fields are two different populations. The fact that some phylogenetic groups shared isolates from both populations, that heterocarion formation was detected between isolates from both populations, and that isolates from soils and diseased plants shared the same haplotype, suggests that infected runner plants and soils could be potential sources of inoculum of FSSC in fruit production fields.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 28966
  AU  - de la Lastra,Eduardo 
  AU  - Villarino,Maria 
  AU  - Astacio,Juan Diego 
  AU  - Larena,Inmaculada 
  AU  - De Cal,Antonieta 
  AU  - Capote,Nieves 
  T1  - Genetic diversity and vegetative compatibility of?Fusarium solani species complex of strawberry in Spain
  PY  - 2018
  KW  - FSSC
  KW  - Fusarium MLST
  KW  - phylogenetic analysis
  KW  - VCG
  KW  - nursery
  KW  - fruit production fields
  UR  - http://dx.doi.org/
  N2  - Fusarium solani is a soil-borne fungus that is pathogen on more than one hundred plants. It is the causal agent of crown and root rot in strawberry. We collected 101 F. solani isolates from diseased plants and soils from two distinct geographic areas of strawberry production in Spain: plant nurseries located in the North-Center of the country, and fruit production fields located in the South-West. Multilocus DNA sequences were constructed for the molecular identification of the isolates via Fusarium MLST online database, and to assess the phylogenetic relationships and diversity of the populations. In addition, the vegetative compatibility between isolates was analyzed based on complementation tests involving nitrate non-utilizing (nit) mutants. Phylogenetic analysis revealed that all strawberry-associated isolates were restricted to Clade 3 of FSSC. Forty-nine isolates from nursery population comprised of 4 distinct phylogenetic species i.e., FSSC 2 (F. keratoplasticum), FSSC 5 (F. solani sensu stricto), FSSC 9, and an unknown FSSC species, that matched with 5 vegetative compatibility groups (VCGs). Fifty-two isolates from fruit production fields comprised 5 strongly supported phylogenetic species i.e., FSSC 2, FSSC 3+4 (F. falciforme), FSSC 5, FSSC 6 (F. metavorans sp. nov.), and FSSC 11, distributed into 31 VCGs. Genetic and VCG variability of fruit production fields population was higher than those from nurseries. Genetic diversity analyses suggested that nurseries and fruit production fields are two different populations. The fact that some phylogenetic groups shared isolates from both populations, that heterocarion formation was detected between isolates from both populations, and that isolates from soils and diseased plants shared the same haplotype, suggests that infected runner plants and soils could be potential sources of inoculum of FSSC in fruit production fields.
  L3  - 
  JF  - Phytopathology
  VL  - 
  IS  - 
  ER  -