@ARTICLE{TreeBASE2Ref16328,
author = {Laurene Levy and Lisa A. Castlebury and L. M Carris and R. J. Meyer and Guillermo Pimentel},
title = {Internal Transcribed Spacer Sequence-Based Phylogeny and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Differentiation of Tilletia walkeri and T. indica.},
year = {2001},
keywords = {},
doi = {10.1094/PHYTO.2001.91.10.935},
url = {},
pmid = {},
journal = {Phytopathology},
volume = {91},
number = {10},
pages = {935--940},
abstract = {A polymerase chain reaction-restriction fragment length polymorphism assay to distinguish Tilleita walkeri, a rye grass bunt fungus that occurs in the southeastern United States and Oregon, from T. indica, the Karnal bunt fungus, is described. The internal transcribed spacer (ITS) region of the ribosomal DNA repeat unit was amplified and sequenced for isolates of T. indica, T. walkeri, T. horrida, and a number of other taxa in the genus Tilletia. A unique restriction digest site in the ITS1 region of T. walkeri was identified that distinguishes it from the other taxa in the genus. Phylogenetic analysis of the taxa based on ITS sequence data revealed a close relationship between T. indica and T. walkeri, but more distant relationships between these two species and other morphologically similar taxa.}
}
Citation for Study 765
Citation title:
"Internal Transcribed Spacer Sequence-Based Phylogeny and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Differentiation of Tilletia walkeri and T. indica.".
This study was previously identified under the legacy study ID S618
(Status: Published).
Citation
Levy L., Castlebury L., M carris L., Meyer R., & Pimentel G. 2001. Internal Transcribed Spacer Sequence-Based Phylogeny and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Differentiation of Tilletia walkeri and T. indica. Phytopathology, 91(10): 935-940.
Authors
-
Levy L.
-
Castlebury L.
-
M carris L.
-
Meyer R.
-
Pimentel G.
Abstract
A polymerase chain reaction-restriction fragment length polymorphism assay to distinguish Tilleita walkeri, a rye grass bunt fungus that occurs in the southeastern United States and Oregon, from T. indica, the Karnal bunt fungus, is described. The internal transcribed spacer (ITS) region of the ribosomal DNA repeat unit was amplified and sequenced for isolates of T. indica, T. walkeri, T. horrida, and a number of other taxa in the genus Tilletia. A unique restriction digest site in the ITS1 region of T. walkeri was identified that distinguishes it from the other taxa in the genus. Phylogenetic analysis of the taxa based on ITS sequence data revealed a close relationship between T. indica and T. walkeri, but more distant relationships between these two species and other morphologically similar taxa.
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S765
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref16328,
author = {Laurene Levy and Lisa A. Castlebury and L. M Carris and R. J. Meyer and Guillermo Pimentel},
title = {Internal Transcribed Spacer Sequence-Based Phylogeny and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Differentiation of Tilletia walkeri and T. indica.},
year = {2001},
keywords = {},
doi = {10.1094/PHYTO.2001.91.10.935},
url = {},
pmid = {},
journal = {Phytopathology},
volume = {91},
number = {10},
pages = {935--940},
abstract = {A polymerase chain reaction-restriction fragment length polymorphism assay to distinguish Tilleita walkeri, a rye grass bunt fungus that occurs in the southeastern United States and Oregon, from T. indica, the Karnal bunt fungus, is described. The internal transcribed spacer (ITS) region of the ribosomal DNA repeat unit was amplified and sequenced for isolates of T. indica, T. walkeri, T. horrida, and a number of other taxa in the genus Tilletia. A unique restriction digest site in the ITS1 region of T. walkeri was identified that distinguishes it from the other taxa in the genus. Phylogenetic analysis of the taxa based on ITS sequence data revealed a close relationship between T. indica and T. walkeri, but more distant relationships between these two species and other morphologically similar taxa.}
}
- Show RIS reference
TY - JOUR
ID - 16328
AU - Levy,Laurene
AU - Castlebury,Lisa A.
AU - M Carris,L.
AU - Meyer,R. J.
AU - Pimentel,Guillermo
T1 - Internal Transcribed Spacer Sequence-Based Phylogeny and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Differentiation of Tilletia walkeri and T. indica.
PY - 2001
KW -
UR - http://dx.doi.org/10.1094/PHYTO.2001.91.10.935
N2 - A polymerase chain reaction-restriction fragment length polymorphism assay to distinguish Tilleita walkeri, a rye grass bunt fungus that occurs in the southeastern United States and Oregon, from T. indica, the Karnal bunt fungus, is described. The internal transcribed spacer (ITS) region of the ribosomal DNA repeat unit was amplified and sequenced for isolates of T. indica, T. walkeri, T. horrida, and a number of other taxa in the genus Tilletia. A unique restriction digest site in the ITS1 region of T. walkeri was identified that distinguishes it from the other taxa in the genus. Phylogenetic analysis of the taxa based on ITS sequence data revealed a close relationship between T. indica and T. walkeri, but more distant relationships between these two species and other morphologically similar taxa.
L3 - 10.1094/PHYTO.2001.91.10.935
JF - Phytopathology
VL - 91
IS - 10
SP - 935
EP - 940
ER -