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Citation for Study 10398

Citation title: "A six-locus phylogeny reveals high levels of species diversity in Botryosphaeriaceae from California almond".

Study name: "A six-locus phylogeny reveals high levels of species diversity in Botryosphaeriaceae from California almond".

This study is part of submission 10388 (Status: Published).

Citation

Inderbitzin P., Bostock R.M., Trouillas F., & Michailides T.J. 2010. A six-locus phylogeny reveals high levels of species diversity in Botryosphaeriaceae from California almond. Mycologia, .

Authors

• Inderbitzin P. (submitter) 530 752 8015
• Bostock R.M.
• Trouillas F.
• Michailides T.J.

Abstract

The Botryosphaeriaceae are important pathogens on a variety of woody hosts, including almond, a major crop in California. Almond is susceptible to Botryosphaeria dothidea that forms band cankers on almond trunks, and the same fungus is also isolated from cankers of the canopy. In order to study the diversity and host range of B. dothidea and allied species from almond, we used 132 isolates from 36 different plant hosts from five continents, including 45 strains from almond in California. Species were identified by comparison to 13 ex-type strains using phylogenetic analyses based on six loci, including the internal transcribed spacer (ITS) regions of the nuclear ribosomal RNA gene repeat, and portions of the coding genes elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase, heat shock protein, histone-3 and beta-tubulin. A total of seven species were found from almond: Botryosphaeria dothidea, Neofusicoccum parvum, Neof. mediterraneum, Neof. nonquaesitum, Diplodia seriata and Macrophomina phaseolina were identified from band cankers, and B. dothidea, Neof. mediterraneum, Neof. parvum and Dothiorella sarmentorum from canopy cankers. All were capable of inducing cankers on inoculated almond branches in the field. All species found on almond also occurred on other hosts suggesting that infected vegetation adjacent to almond orchards could serve as source of inoculum of virulent almond strains. Of the 19 monophyletic groups obtained at the species level, thirteen contained ex-type strains, five were morphologically similar to established species, and one was morphologically distinct from its closest relatives Neof. andinum and Neof. arbuti, as well as from the more than 190 described species of Fusicoccum and Neofusicoccum, and was thus described as the new species Neof. nonquaesitum. Evidence for cryptic speciation was found in B. dothidea, Neof. ribis and Spencermartinsia viticola. Botryosphaeria dothidea and Neof. ribis comprised lineages that formed the morphologically distinct Dichomera anamorph not found in any other isolates recognized as B. dothidea and Neof. ribis. A S. viticola isolate from California was phylogenetically divergent, and had conidia that differed morphologically from the type. Neofusicoccum parvum was diverse, but lacked any morphological features correlating with molecular diversity. Phylogenetic analyses of different combinations of datasets showed that pooled analyses of all six datasets resulted in the highest number of supported branches, suggesting that addition of more data might yet improve phylogenetic resolution.

Keywords

Lasiodiplodia theobromae, D. mutila, Neoscytalidium dimidiatum, Do. iberica, Dich. eucalypti, Neof. australe, Neof. luteum, Dich. versiformis.

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S10398
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref18859,
  	 author = {Patrik  Inderbitzin and Richard M Bostock and Florent P.  Trouillas and Themis J Michailides},
  	 title = {A six-locus phylogeny reveals high levels of species diversity in Botryosphaeriaceae from California almond},
  	 year = {2010},
  	 keywords = {Lasiodiplodia theobromae, D. mutila, Neoscytalidium dimidiatum, Do. iberica, Dich. eucalypti, Neof. australe, Neof. luteum, Dich. versiformis. },
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Mycologia},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {The Botryosphaeriaceae are important pathogens on a variety of woody hosts, including almond, a major crop in California. Almond is susceptible to Botryosphaeria dothidea that forms band cankers on almond trunks, and the same fungus is also isolated from cankers of the canopy. In order to study the diversity and host range of B. dothidea and allied species from almond, we used 132 isolates from 36 different plant hosts from five continents, including 45 strains from almond in California. Species were identified by comparison to 13 ex-type strains using phylogenetic analyses based on six loci, including the internal transcribed spacer (ITS) regions of the nuclear ribosomal RNA gene repeat, and portions of the coding genes elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase, heat shock protein, histone-3 and beta-tubulin. A total of seven species were found from almond: Botryosphaeria dothidea, Neofusicoccum parvum, Neof. mediterraneum, Neof. nonquaesitum, Diplodia seriata and Macrophomina phaseolina were identified from band cankers, and B. dothidea, Neof. mediterraneum, Neof. parvum and Dothiorella sarmentorum from canopy cankers. All were capable of inducing cankers on inoculated almond branches in the field. All species found on almond also occurred on other hosts suggesting that infected vegetation adjacent to almond orchards could serve as source of inoculum of virulent almond strains. Of the 19 monophyletic groups obtained at the species level, thirteen contained ex-type strains, five were morphologically similar to established species, and one was morphologically distinct from its closest relatives Neof. andinum and Neof. arbuti, as well as from the more than 190 described species of Fusicoccum and Neofusicoccum, and was thus described as the new species Neof. nonquaesitum. Evidence for cryptic speciation was found in B. dothidea, Neof. ribis and Spencermartinsia viticola. Botryosphaeria dothidea and Neof. ribis comprised lineages that formed the morphologically distinct Dichomera anamorph not found in any other isolates recognized as B. dothidea and Neof. ribis. A S. viticola isolate from California was phylogenetically divergent, and had conidia that differed morphologically from the type. Neofusicoccum parvum was diverse, but lacked any morphological features correlating with molecular diversity. Phylogenetic analyses of different combinations of datasets showed that pooled analyses of all six datasets resulted in the highest number of supported branches, suggesting that addition of more data might yet improve phylogenetic resolution.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 18859
  AU  - Inderbitzin,Patrik 
  AU  - Bostock,Richard M
  AU  - Trouillas,Florent P. 
  AU  - Michailides,Themis J
  T1  - A six-locus phylogeny reveals high levels of species diversity in Botryosphaeriaceae from California almond
  PY  - 2010
  KW  - Lasiodiplodia theobromae
  KW  - D. mutila
  KW  - Neoscytalidium dimidiatum
  KW  - Do. iberica
  KW  - Dich. eucalypti
  KW  - Neof. australe
  KW  - Neof. luteum
  KW  - Dich. versiformis. 
  UR  - http://dx.doi.org/
  N2  - The Botryosphaeriaceae are important pathogens on a variety of woody hosts, including almond, a major crop in California. Almond is susceptible to Botryosphaeria dothidea that forms band cankers on almond trunks, and the same fungus is also isolated from cankers of the canopy. In order to study the diversity and host range of B. dothidea and allied species from almond, we used 132 isolates from 36 different plant hosts from five continents, including 45 strains from almond in California. Species were identified by comparison to 13 ex-type strains using phylogenetic analyses based on six loci, including the internal transcribed spacer (ITS) regions of the nuclear ribosomal RNA gene repeat, and portions of the coding genes elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase, heat shock protein, histone-3 and beta-tubulin. A total of seven species were found from almond: Botryosphaeria dothidea, Neofusicoccum parvum, Neof. mediterraneum, Neof. nonquaesitum, Diplodia seriata and Macrophomina phaseolina were identified from band cankers, and B. dothidea, Neof. mediterraneum, Neof. parvum and Dothiorella sarmentorum from canopy cankers. All were capable of inducing cankers on inoculated almond branches in the field. All species found on almond also occurred on other hosts suggesting that infected vegetation adjacent to almond orchards could serve as source of inoculum of virulent almond strains. Of the 19 monophyletic groups obtained at the species level, thirteen contained ex-type strains, five were morphologically similar to established species, and one was morphologically distinct from its closest relatives Neof. andinum and Neof. arbuti, as well as from the more than 190 described species of Fusicoccum and Neofusicoccum, and was thus described as the new species Neof. nonquaesitum. Evidence for cryptic speciation was found in B. dothidea, Neof. ribis and Spencermartinsia viticola. Botryosphaeria dothidea and Neof. ribis comprised lineages that formed the morphologically distinct Dichomera anamorph not found in any other isolates recognized as B. dothidea and Neof. ribis. A S. viticola isolate from California was phylogenetically divergent, and had conidia that differed morphologically from the type. Neofusicoccum parvum was diverse, but lacked any morphological features correlating with molecular diversity. Phylogenetic analyses of different combinations of datasets showed that pooled analyses of all six datasets resulted in the highest number of supported branches, suggesting that addition of more data might yet improve phylogenetic resolution.
  L3  - 
  JF  - Mycologia
  VL  - 
  IS  - 
  ER  -